FastQCFastQC Report
Thu 2 Feb 2017
SRR4062879_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062879_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2447111
Sequences flagged as poor quality0
Sequence length38
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA173790.7101843765975471No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA163640.6687068956005674No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG147470.6026289775984824No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC134860.5510988263303136No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA89330.3650426972867189No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA74560.30468581114628634No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA69310.28323194166508997No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA68460.27975845803480104No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA61280.2504177374871839No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT60620.24772067960954774No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT54440.22246641039168225No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA50620.20685616631203077No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAG39580.1617417436315721No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT39430.16112877593210934No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACG36400.1487468284029617No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTT36080.14743916397744117No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCA36010.14715311238435855No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG35440.14482383512640007No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT35110.14347530618758203No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAA34590.14135035149611114No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGT33570.1371821711397644No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTC32520.1328913972435251No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAAT31260.12774246856803798No Hit
CACTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAA29900.12218489475957568No Hit
ACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT29640.12112241741384024No Hit
CCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA29120.11899746272236937No Hit
GAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTT27510.11241827608146912No Hit
GACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAA27260.11139666324903119No Hit
ATACACACTTTAGGACGTGAAATATGGCGAGGAAAACT26640.10886306342458515No Hit
CCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGT26470.10816836669852736No Hit
ACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAA26270.10735107643257702No Hit
AGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCT25240.10314203156293276No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAA25170.10285597996985017No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG308.4226707E-426.6649978
TCTTCGC451.2386319E-524.88936629
TAGGACC42600.022.3803734
CACGGTT1151.8189894E-1220.86911228
GAACGGA555.8377147E-520.36319426
CGCGTTT400.004485464719.99956728
TTCGCGT400.004485464719.99956726
TCGCGTT400.004485464719.99956727
ACCGTCG1650.019.3927278
AGGACCT81300.018.8300745
TACCGTC1700.018.8219667
CGACAAC601.1390391E-418.66626219
GACGTGC601.13957474E-418.6651177
CTACGTC601.1406468E-418.6628284
ATACCGT1800.018.6628286
AGCGGCG1550.018.58100131
GGACCTG81900.018.3014876
CTAGCGG1600.018.00034529
GACGTTA802.80102E-617.9985077
GTCCTAG6200.017.822821
Produced by FastQC (version 0.11.5)