##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062879_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2447111
Sequences flagged as poor quality	0
Sequence length	38
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.317813536043115	32.0	32.0	32.0	32.0	32.0
2	31.029796768516018	32.0	32.0	32.0	32.0	32.0
3	31.05438862397333	32.0	32.0	32.0	32.0	32.0
4	31.109411465193038	32.0	32.0	32.0	32.0	32.0
5	31.09524945946465	32.0	32.0	32.0	32.0	32.0
6	34.760634887424395	36.0	36.0	36.0	32.0	36.0
7	34.68737421392001	36.0	36.0	36.0	32.0	36.0
8	34.67960954774834	36.0	36.0	36.0	32.0	36.0
9	34.77317784113593	36.0	36.0	36.0	32.0	36.0
10	34.55250415694262	36.0	36.0	36.0	32.0	36.0
11	34.751714164171545	36.0	36.0	36.0	32.0	36.0
12	34.61947700778592	36.0	36.0	36.0	32.0	36.0
13	34.66862353199344	36.0	36.0	36.0	32.0	36.0
14	34.602780176297685	36.0	36.0	36.0	32.0	36.0
15	34.577277042193835	36.0	36.0	36.0	32.0	36.0
16	34.61206336778348	36.0	36.0	36.0	32.0	36.0
17	34.596221421913434	36.0	36.0	36.0	32.0	36.0
18	34.627835026690654	36.0	36.0	36.0	32.0	36.0
19	34.52401791336805	36.0	36.0	36.0	32.0	36.0
20	34.502453301055816	36.0	36.0	36.0	32.0	36.0
21	34.449994299400394	36.0	36.0	36.0	32.0	36.0
22	34.408018271341184	36.0	36.0	36.0	32.0	36.0
23	34.425359127558984	36.0	36.0	36.0	32.0	36.0
24	34.387315082969266	36.0	36.0	36.0	32.0	36.0
25	34.35344616570315	36.0	36.0	36.0	32.0	36.0
26	34.367998836178664	36.0	36.0	36.0	32.0	36.0
27	34.28598784444188	36.0	36.0	36.0	32.0	36.0
28	34.17956561839655	36.0	36.0	36.0	32.0	36.0
29	34.106122280517724	36.0	36.0	36.0	32.0	36.0
30	34.03916414089921	36.0	36.0	36.0	32.0	36.0
31	34.03701139833869	36.0	36.0	36.0	32.0	36.0
32	34.013812614139695	36.0	36.0	36.0	32.0	36.0
33	34.077182440845554	36.0	36.0	36.0	32.0	36.0
34	34.0238084827374	36.0	36.0	36.0	32.0	36.0
35	34.0318722771464	36.0	36.0	36.0	32.0	36.0
36	33.92630943181572	36.0	36.0	36.0	32.0	36.0
37	33.82597029721986	36.0	36.0	36.0	32.0	36.0
38	33.26680032086816	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	2.0
9	6.0
10	21.0
11	23.0
12	12.0
13	12.0
14	121.0
15	371.0
16	560.0
17	681.0
18	825.0
19	1004.0
20	1392.0
21	2058.0
22	3078.0
23	4880.0
24	7776.0
25	12356.0
26	19339.0
27	28762.0
28	41592.0
29	58557.0
30	81179.0
31	109803.0
32	149666.0
33	228615.0
34	549918.0
35	1144498.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.17457153951182	21.0925266801989	13.10820890484243	25.624692875446854
2	10.60129053571706	20.504242773138998	50.95661716423952	17.937849526904422
3	18.35775603994548	27.237676426894335	31.072385346784724	23.332182186375462
4	10.0982947879202	18.055092976088943	41.563701585638405	30.282910650352445
5	9.355192123251145	41.43776656541761	37.91506766578085	11.291973645550387
6	27.862649074170935	40.456310806569725	20.164529174076787	11.516510945182551
7	25.23257016130449	32.63309265497151	24.16621068680579	17.968126496918202
8	20.554973916127867	46.38642543477888	20.482602715207268	12.575997933885985
9	26.757034636014946	15.036577900589512	22.137904937505773	36.06848252588976
10	15.238696922355818	29.512169307412574	36.78633353207206	18.462800238159552
11	32.608138641075215	19.70407839262268	31.585812730096883	16.101970236205222
12	26.6351511941716	23.03772048979752	35.98579024883819	14.341338067192687
13	30.90490430842005	24.23148587503428	26.529497904786194	18.334111911759475
14	17.257928134809656	27.458521787070673	27.939295929889692	27.344254148229975
15	17.88407301740417	43.3419441333475	22.152468383921782	16.62151446532654
16	16.00303737706633	28.13730154059427	37.89115629577318	17.96850478656623
17	16.94502233684701	33.4407736355722	32.636769760854726	16.977434266726068
18	17.381423514478627	26.5796780202257	41.10910319879231	14.929795266503366
19	24.133527208765916	25.087292985665314	28.509701127779604	22.269478677789166
20	23.92657041157023	29.62471955111832	31.12886549487734	15.319844542434113
21	19.867811920711674	27.204156327340613	27.323732880855	25.604298871092716
22	22.640767652660056	34.82410684364962	27.73978928784523	14.795336215845095
23	16.513812084700554	34.14827623270297	32.20702780971188	17.1308838728846
24	23.406924050260425	27.561534034991354	33.33067677629665	15.700865138451578
25	19.33632673346641	31.035232532096085	33.24762661461322	16.380814119824286
26	14.642930858662131	33.57956066548001	34.705833065961485	17.07167540989637
27	16.99712001883206	32.688092767516004	31.963745013570282	18.351042200081654
28	15.792220360060597	33.7447797833817	28.841627853469813	21.62137200308789
29	19.810785604698932	29.677005657060047	27.826588754376026	22.685619983864996
30	16.13206949606566	30.419465109959155	31.831290393106787	21.617175000868393
31	22.78905198321851	27.201180883226765	30.4902528911124	19.519514242442323
32	18.077934221264663	32.72671693582366	31.04606080767123	18.149288035240446
33	15.52334900204911	33.28788833608374	35.33368970057795	15.8550729612892
34	18.335551192494375	32.1297905349182	34.06055409423885	15.474104178348572
35	16.586388632875643	33.5774848214231	33.20097845878436	16.635148086916896
36	15.27360561066222	31.624263825369773	35.171307478839445	17.93082308512856
37	16.508280970625798	31.841103448275867	28.76432183908046	22.88629374201788
38	18.297924336845014	35.922160281849806	28.487680922215453	17.292234459089727
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	365.0
1	331.5
2	298.0
3	298.0
4	705.0
5	1112.0
6	1112.0
7	1407.0
8	1702.0
9	1672.0
10	1642.0
11	1642.0
12	2304.0
13	2966.0
14	4201.5
15	5437.0
16	5437.0
17	8509.5
18	11582.0
19	11582.0
20	15851.0
21	20120.0
22	27449.5
23	34779.0
24	34779.0
25	54529.5
26	74280.0
27	74280.0
28	105221.0
29	136162.0
30	192132.0
31	248102.0
32	248102.0
33	306448.0
34	364794.0
35	364794.0
36	375086.5
37	385379.0
38	375264.5
39	365150.0
40	365150.0
41	309226.5
42	253303.0
43	202716.5
44	152130.0
45	152130.0
46	128050.5
47	103971.0
48	103971.0
49	90172.5
50	76374.0
51	66070.0
52	55766.0
53	55766.0
54	47971.5
55	40177.0
56	40177.0
57	36243.5
58	32310.0
59	27098.5
60	21887.0
61	21887.0
62	21086.0
63	20285.0
64	16324.5
65	12364.0
66	12364.0
67	10545.5
68	8727.0
69	8727.0
70	7088.5
71	5450.0
72	4302.0
73	3154.0
74	3154.0
75	2373.0
76	1592.0
77	1592.0
78	1617.5
79	1643.0
80	1385.0
81	1127.0
82	1127.0
83	1119.5
84	1112.0
85	1112.0
86	774.5
87	437.0
88	354.0
89	271.0
90	271.0
91	203.5
92	136.0
93	124.5
94	113.0
95	113.0
96	109.5
97	106.0
98	106.0
99	456.0
100	806.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12545405582337704
2	0.05332818985325962
3	0.011278605670114678
4	0.003432619116991424
5	4.0864513297516947E-4
6	2.8605159308261866E-4
7	0.0
8	2.0432256648758473E-4
9	2.0432256648758473E-4
10	4.903741595702034E-4
11	0.0017980385850907459
12	0.0024110062845535
13	0.01405739257434583
14	0.009153650978643797
15	0.02333363709288218
16	0.010747366997246956
17	0.019655830896105654
18	0.005108064162189619
19	0.007314747880255534
20	0.00576189637494989
21	0.005843625401544924
22	0.006987831773875398
23	0.009480567085023931
24	0.013689611954668179
25	0.016141482752519195
26	0.01863421806366773
27	0.008990192925453729
28	0.005475844781867271
29	0.010502179917461855
30	0.0026970578776361184
31	0.005271522215379686
32	0.006129676994627542
33	0.008050309119610839
34	0.012545405582337703
35	0.01659099239879188
36	0.013934799034453278
37	0.009644025138214
38	0.005475844781867271
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2447111.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.73017884985784
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.34184838765822	39.63802819908606
2	11.291824596686862	11.005052642755492
3	3.025874239846144	4.423541786546407
4	1.2328335687012177	2.4030480117967534
5	0.6839499995805733	1.6664502901960772
6	0.4332588294123338	1.2667668147325843
7	0.2940967681167519	1.0031971676646145
8	0.21347852584319443	0.8322277395954292
9	0.1659685787919423	0.7278910675189264
>10	1.088671498013774	10.235015360474542
>50	0.1194025012802554	4.074070767621732
>100	0.09177498297941812	8.818067717091008
>500	0.00918272285509011	3.0383601867185104
>1k	0.006823858268461441	6.153858620330634
>5k	6.739613104653275E-4	2.1701341177705147
>10k+	3.3698065523266377E-4	2.544289510100742
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA	17379	0.7101843765975471	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	16364	0.6687068956005674	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG	14747	0.6026289775984824	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	13486	0.5510988263303136	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	8933	0.3650426972867189	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA	7456	0.30468581114628634	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	6931	0.28323194166508997	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA	6846	0.27975845803480104	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA	6128	0.2504177374871839	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT	6062	0.24772067960954774	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5444	0.22246641039168225	No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	5062	0.20685616631203077	No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAG	3958	0.1617417436315721	No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT	3943	0.16112877593210934	No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACG	3640	0.1487468284029617	No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTT	3608	0.14743916397744117	No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCA	3601	0.14715311238435855	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG	3544	0.14482383512640007	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3511	0.14347530618758203	No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAA	3459	0.14135035149611114	No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGT	3357	0.1371821711397644	No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTC	3252	0.1328913972435251	No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAAT	3126	0.12774246856803798	No Hit
CACTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAA	2990	0.12218489475957568	No Hit
ACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT	2964	0.12112241741384024	No Hit
CCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	2912	0.11899746272236937	No Hit
GAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTT	2751	0.11241827608146912	No Hit
GACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAA	2726	0.11139666324903119	No Hit
ATACACACTTTAGGACGTGAAATATGGCGAGGAAAACT	2664	0.10886306342458515	No Hit
CCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGT	2647	0.10816836669852736	No Hit
ACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAA	2627	0.10735107643257702	No Hit
AGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCT	2524	0.10314203156293276	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAA	2517	0.10285597996985017	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	4.086451329751695E-5	0.0
15	0.0	0.0	0.0	8.17290265950339E-5	0.0
16	0.0	0.0	0.0	8.17290265950339E-5	0.0
17	0.0	0.0	0.0	8.17290265950339E-5	0.0
18	0.0	0.0	0.0	1.2259353989255086E-4	0.0
19	0.0	0.0	0.0	2.0432256648758476E-4	0.0
20	0.0	0.0	0.0	3.6778061967765257E-4	0.0
21	0.0	0.0	0.0	5.312386728677204E-4	0.0
22	0.0	0.0	0.0	6.946967260577882E-4	0.0
23	0.0	0.0	0.0	0.0011850708856279916	0.0
24	0.0	0.0	0.0	0.0018389030983882628	0.0
25	0.0	0.0	0.0	0.002370141771255983	0.0
26	0.0	0.0	0.0	0.0027787869042311527	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTGCG	30	8.4226707E-4	26.664997	8
TCTTCGC	45	1.2386319E-5	24.889366	29
TAGGACC	4260	0.0	22.380373	4
CACGGTT	115	1.8189894E-12	20.869112	28
GAACGGA	55	5.8377147E-5	20.363194	26
CGCGTTT	40	0.0044854647	19.999567	28
TTCGCGT	40	0.0044854647	19.999567	26
TCGCGTT	40	0.0044854647	19.999567	27
ACCGTCG	165	0.0	19.392727	8
AGGACCT	8130	0.0	18.830074	5
TACCGTC	170	0.0	18.821966	7
CGACAAC	60	1.1390391E-4	18.666262	19
GACGTGC	60	1.13957474E-4	18.665117	7
CTACGTC	60	1.1406468E-4	18.662828	4
ATACCGT	180	0.0	18.662828	6
AGCGGCG	155	0.0	18.581001	31
GGACCTG	8190	0.0	18.301487	6
CTAGCGG	160	0.0	18.000345	29
GACGTTA	80	2.80102E-6	17.998507	7
GTCCTAG	620	0.0	17.82282	1
>>END_MODULE