FastQCFastQC Report
Thu 2 Feb 2017
SRR4062879_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062879_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2447111
Sequences flagged as poor quality0
Sequence length38
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA169090.6909780553477141No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA161690.6607383155075516No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG158640.6482746389518089No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC129820.530503111628365No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA94170.3848211217227171No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA82340.33647840249175454No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA73170.29900564379793154No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT65470.2675399685588435No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA64210.2623910398833563No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA55500.22679804880121907No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA55030.22487741667623576No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT54860.22418271995017797No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAG44920.18356339373244615No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT42220.17252997514211654No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACG37630.15377316353855627No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT37420.1529150087593084No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG37190.15197512495346552No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTT36720.15005449282848224No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCA36330.14846077680987907No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTC34700.14179986114238383No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGT34330.1402878741503757No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAA33920.1386124291051775No Hit
CCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA32800.1340356036158556No Hit
CACTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAA32300.13199237795097973No Hit
ACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT31780.12986742325950887No Hit
ACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAA31540.12888667494036846No Hit
GAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTT31510.1287640814004759No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAAT30250.12361515272498877No Hit
GACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAA29990.12255267537925334No Hit
ATACACACTTTAGGACGTGAAATATGGCGAGGAAAACT29270.1196104304218321No Hit
CCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGT28040.11458409528623753No Hit
GAAATACACACTTTAGGACGTGAAATATGGCGAGGAAA27800.11360334696709712No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAA27160.11098801811605605No Hit
AGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCT25650.10481747660813097No Hit
TCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAG24720.1010170768714619No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGGCG805.2641553E-921.99893216
TAGGACC43850.021.456114
TTGCCGT604.726151E-621.33229824
CGCTTCG1250.020.47900632
TAGCGTA801.2651617E-720.0027087
CGAGAAC1203.6379788E-1219.9990321
AGGACCT84500.018.4076025
AACGCTT1401.8189894E-1218.28482830
TGCCGTG701.78935E-518.28482825
TTCGGAA1950.018.05040631
ATAGCGT802.794639E-618.0028066
GGACCTG85050.017.7805486
TCATCGC450.00885552517.77691529
GGTCTAC1453.6379788E-1217.6572021
AGCGGCG1453.6379788E-1217.65431631
TACGTCC1006.811388E-817.6027435
ACCGTCG1006.812843E-817.6023858
TACCGTC1006.812843E-817.6023857
CCGTCGT1006.81448E-817.6020229
CACGGTT1006.827213E-817.59914628
Produced by FastQC (version 0.11.5)