FastQCFastQC Report
Thu 2 Feb 2017
SRR4062878_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062878_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1122165
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT40180.3580578613661984No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT28040.2498741272451021No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG17430.15532475170763657No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC17310.1542553902500969No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG17190.15318602879255724No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG17140.15274046151858237No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT17030.15176021351583768No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC16440.14650251968293432No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC16220.14454202367744495No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA16210.14445291022264994No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA16200.144363796767855No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG15520.13830408184179688No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG14810.13197702655135385No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT14130.12591731162529574No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA14060.12529351744173092No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT14010.12484795016775609No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT13350.11896646215128791No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA12880.11477812977592422No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA12850.1145107894115393No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12520.11157004540330522No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT12470.11112447812933036No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG12190.10862930139507114No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG11870.10577767084163203No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT11820.10533210356765715No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC11770.10488653629368229No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT11650.10381717483614263No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC11610.10346072101696274No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC11460.10212401919503816No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT11370.10132199810188341No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACC851.0581061E-820.7031144
ATAGGCG400.00448804219.9964375
CTAAGAC659.433485E-619.6923083
TACTAGA601.1354314E-418.6724912
ACACCGT802.8008253E-617.9967926
ATACTAG450.00880027217.7952251
GTACCTA550.001366249217.4514356
TTAATAC753.2335796E-517.0666683
ATCGTTT4000.016.3992729
GTCTAGG1304.7384674E-916.0157031
ATAGATC805.620667E-516.03
TACCGTC2700.015.9978617
ATACCGT2800.015.4258216
GTATTAG3650.015.3575231
CGCTTCG3650.015.34178332
GATATAC1153.307523E-715.3193671
ACGCTTC3800.015.15721931
GTCCTAC859.353312E-515.0736031
GTCTAGA859.353312E-515.0736031
CCGTGTG859.435454E-515.0581519
Produced by FastQC (version 0.11.5)