##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062878_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1122165
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.146506975355674	32.0	32.0	32.0	32.0	32.0
2	30.741112046802385	32.0	32.0	32.0	32.0	32.0
3	30.79816069829303	32.0	32.0	32.0	32.0	32.0
4	30.85470853216773	32.0	32.0	32.0	32.0	32.0
5	30.776618411730894	32.0	32.0	32.0	32.0	32.0
6	34.40538067040052	36.0	36.0	36.0	32.0	36.0
7	34.319690063404224	36.0	36.0	36.0	32.0	36.0
8	34.29886603128774	36.0	36.0	36.0	32.0	36.0
9	34.436945547223445	36.0	36.0	36.0	32.0	36.0
10	34.150420838290266	36.0	36.0	36.0	32.0	36.0
11	34.44754202813312	36.0	36.0	36.0	32.0	36.0
12	34.23838472951839	36.0	36.0	36.0	32.0	36.0
13	34.34224289654374	36.0	36.0	36.0	32.0	36.0
14	34.22316682484305	36.0	36.0	36.0	32.0	36.0
15	34.16752973047635	36.0	36.0	36.0	32.0	36.0
16	34.17964381352118	36.0	36.0	36.0	32.0	36.0
17	34.09426866815486	36.0	36.0	36.0	32.0	36.0
18	34.12741619993495	36.0	36.0	36.0	32.0	36.0
19	34.11918122557734	36.0	36.0	36.0	32.0	36.0
20	34.101172287497825	36.0	36.0	36.0	32.0	36.0
21	34.0758872358343	36.0	36.0	36.0	32.0	36.0
22	34.062416846007494	36.0	36.0	36.0	32.0	36.0
23	34.014483609807826	36.0	36.0	36.0	32.0	36.0
24	33.98419661992666	36.0	36.0	36.0	32.0	36.0
25	33.96586776454443	36.0	36.0	36.0	32.0	36.0
26	33.91913043090811	36.0	36.0	36.0	32.0	36.0
27	33.927433131491355	36.0	36.0	36.0	32.0	36.0
28	33.90599154313314	36.0	36.0	36.0	32.0	36.0
29	33.87501570624641	36.0	36.0	36.0	32.0	36.0
30	33.84716240481569	36.0	36.0	36.0	32.0	36.0
31	33.8683250680604	36.0	36.0	36.0	32.0	36.0
32	33.8133884054484	36.0	36.0	36.0	32.0	36.0
33	33.75895612499053	36.0	36.0	36.0	32.0	36.0
34	33.76677493951424	36.0	36.0	36.0	32.0	36.0
35	33.71354034388882	36.0	36.0	36.0	27.0	36.0
36	33.66032000641617	36.0	36.0	36.0	27.0	36.0
37	33.66447447567871	36.0	36.0	36.0	27.0	36.0
38	33.04800274469441	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	1.0
9	2.0
10	11.0
11	11.0
12	6.0
13	3.0
14	168.0
15	333.0
16	409.0
17	486.0
18	682.0
19	846.0
20	1132.0
21	1754.0
22	2601.0
23	3972.0
24	5864.0
25	8678.0
26	12854.0
27	18064.0
28	24614.0
29	32672.0
30	43150.0
31	57441.0
32	76346.0
33	112397.0
34	239234.0
35	478431.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.66340983366687	17.30085838709591	11.330424161531303	25.70530761770592
2	16.899243467832896	20.02532176021898	36.92536767819966	26.15006709374847
3	18.514025979813283	24.47039950090239	28.599184510149062	28.416390009135267
4	12.509735983303122	15.263348322737375	35.91744599091368	36.30946970304583
5	14.84856910143099	36.24369743406032	33.19627647345036	15.71145699105833
6	33.95671554273106	35.54214290679209	16.85636069074052	13.644780859736331
7	30.172479091755665	30.555488720464457	21.151256722496246	18.120775465283625
8	28.609480084444062	31.843056668238034	19.846403775565584	19.701059471752323
9	28.06951568576429	13.874353481309326	18.71607002185064	39.340060811075745
10	16.328109196257586	26.016184814505554	30.805150410412747	26.85055557882411
11	37.67753928633172	21.091996941574447	22.15050447980922	19.079959292284602
12	25.288756562244068	23.750890041251573	28.12975932571633	22.83059407078802
13	29.635690170871616	18.885626891690997	25.380120711721442	26.098562225715945
14	24.166234432900385	19.8451444551983	24.811822351030855	31.176798760870454
15	25.59519034503659	26.52619194052999	22.602258648198163	25.27635906623526
16	26.084774961610545	25.389085898794445	23.52117056859168	25.00496857100333
17	24.118647950293287	25.508917135571302	25.203642615788173	25.16879229834724
18	25.494919356707324	24.010473201188486	26.263120464984468	24.231486977119726
19	25.80883486605643	24.756233306626907	25.52400592829191	23.910925899024754
20	26.079761162106763	23.478656091257463	25.10801176365743	25.333570982978344
21	27.80285962798457	23.67997975232242	24.09580573636668	24.421354883326323
22	26.202326897455208	23.640155245322365	24.944857610095404	25.212660247127026
23	24.407839740196312	23.448815501239665	25.66844729154301	26.47489746702101
24	24.863637335919172	24.960784593336232	25.07985682811531	25.09572124262929
25	25.06276877827046	23.97477662158248	25.253058223222443	25.70939637692462
26	24.82637589084855	25.089664195999156	25.770791219682625	24.313168693469667
27	25.63651935380664	24.435445276134747	24.6384612848115	25.289574085247118
28	24.571627179516888	24.19777291584046	25.930513904794157	25.3000859998485
29	24.428566717793462	24.641659395729413	25.84107439349689	25.088699492980243
30	24.53855696121312	24.799667062342042	26.043905708062866	24.61787026838197
31	25.22450987469154	24.723311686633497	24.46487023119816	25.5873082074768
32	24.59682594002652	24.690668178123797	24.49549771142576	26.217008170423924
33	24.409265666023217	24.122030732266385	25.322303797062233	26.146399804648162
34	25.376501057892664	24.35202960316993	25.42783603465824	24.843633304279162
35	26.406449140381994	24.175542107467848	25.26145043270559	24.156558319444567
36	24.718716577540107	25.25035650623886	24.656238859180036	25.374688057041002
37	25.778453119917295	25.074572328697535	24.780115233965947	24.366859317419223
38	24.701721052594056	24.679441477795287	25.324568845668495	25.294268623942166
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	623.0
1	461.0
2	299.0
3	299.0
4	750.0
5	1201.0
6	1201.0
7	1390.0
8	1579.0
9	1482.5
10	1386.0
11	1386.0
12	1796.5
13	2207.0
14	2701.5
15	3196.0
16	3196.0
17	4722.0
18	6248.0
19	6248.0
20	7106.5
21	7965.0
22	7973.0
23	7981.0
24	7981.0
25	9074.5
26	10168.0
27	10168.0
28	12940.5
29	15713.0
30	19224.5
31	22736.0
32	22736.0
33	29772.5
34	36809.0
35	36809.0
36	40650.0
37	44491.0
38	51342.0
39	58193.0
40	58193.0
41	62158.5
42	66124.0
43	76245.0
44	86366.0
45	86366.0
46	89526.0
47	92686.0
48	92686.0
49	98426.0
50	104166.0
51	104656.5
52	105147.0
53	105147.0
54	99889.5
55	94632.0
56	94632.0
57	92859.5
58	91087.0
59	81614.0
60	72141.0
61	72141.0
62	69582.0
63	67023.0
64	55404.5
65	43786.0
66	43786.0
67	36533.5
68	29281.0
69	29281.0
70	23640.5
71	18000.0
72	14195.0
73	10390.0
74	10390.0
75	7785.5
76	5181.0
77	5181.0
78	5307.5
79	5434.0
80	4293.5
81	3153.0
82	3153.0
83	3242.0
84	3331.0
85	3331.0
86	2244.0
87	1157.0
88	987.5
89	818.0
90	818.0
91	594.0
92	370.0
93	335.5
94	301.0
95	301.0
96	272.0
97	243.0
98	243.0
99	398.0
100	553.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11959025633485271
2	0.053468072876983325
3	0.01247588367129611
4	0.0034754247370039166
5	2.6734036438491663E-4
6	6.237941835648055E-4
7	0.0
8	1.7822690958994446E-4
9	8.911345479497223E-5
10	1.7822690958994446E-4
11	0.0016931556411044723
12	0.0023169498246692777
13	0.01363435858363075
14	0.007752870567162584
15	0.022723930972717916
16	0.009980706937036888
17	0.019961413874073776
18	0.00561414765208325
19	0.007841984021957555
20	0.005792374561673194
21	0.005435920742493305
22	0.006237941835648055
23	0.007752870567162584
24	0.013990812402810639
25	0.016931556411044723
26	0.017911804413789416
27	0.007663757112367611
28	0.006237941835648055
29	0.010515387665806722
30	0.0029407440082340835
31	0.0048121265589285
32	0.006148828380853083
33	0.007752870567162584
34	0.010960954939781583
35	0.013812585493220694
36	0.014703720041170416
37	0.00891134547949722
38	0.005435920742493305
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1122165.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.249742797110954
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.22728673244546	40.6037806755079
2	12.591179969600052	12.90589469908276
3	3.7667788978642784	5.79139349067388
4	1.528528451890474	3.1334676006981184
5	0.7852101641407842	2.0120909476946234
6	0.4521346286603983	1.390307005710761
7	0.2954282718681943	1.0598436063767949
8	0.20485520882912578	0.8399021410512918
9	0.16140333432405227	0.7444691433633409
>10	0.7714968704491896	7.236789367724753
>50	0.08998435610427873	3.261045092538415
>100	0.10548742635985438	11.695092261338102
>500	0.01429746872455781	4.841098614509933
>1k	0.005928218739450799	4.484825353729309
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4018	0.3580578613661984	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2804	0.2498741272451021	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1743	0.15532475170763657	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1731	0.1542553902500969	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1719	0.15318602879255724	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1714	0.15274046151858237	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1703	0.15176021351583768	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1644	0.14650251968293432	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1622	0.14454202367744495	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1621	0.14445291022264994	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1620	0.144363796767855	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1552	0.13830408184179688	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1481	0.13197702655135385	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1413	0.12591731162529574	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1406	0.12529351744173092	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1401	0.12484795016775609	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1335	0.11896646215128791	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1288	0.11477812977592422	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1285	0.1145107894115393	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1252	0.11157004540330522	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	1247	0.11112447812933036	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1219	0.10862930139507114	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1187	0.10577767084163203	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1182	0.10533210356765715	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1177	0.10488653629368229	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1165	0.10381717483614263	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	1161	0.10346072101696274	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1146	0.10212401919503816	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT	1137	0.10132199810188341	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	8.911345479497222E-5	0.0
6	0.0	0.0	0.0	8.911345479497222E-5	0.0
7	0.0	0.0	0.0	8.911345479497222E-5	0.0
8	0.0	0.0	0.0	8.911345479497222E-5	0.0
9	0.0	0.0	0.0	8.911345479497222E-5	0.0
10	0.0	0.0	0.0	8.911345479497222E-5	0.0
11	0.0	0.0	0.0	8.911345479497222E-5	0.0
12	0.0	0.0	0.0	8.911345479497222E-5	0.0
13	0.0	0.0	0.0	8.911345479497222E-5	0.0
14	0.0	0.0	0.0	8.911345479497222E-5	0.0
15	0.0	0.0	0.0	2.6734036438491663E-4	0.0
16	0.0	0.0	0.0	2.6734036438491663E-4	0.0
17	0.0	0.0	0.0	2.6734036438491663E-4	0.0
18	0.0	0.0	0.0	2.6734036438491663E-4	0.0
19	0.0	0.0	0.0	2.6734036438491663E-4	0.0
20	0.0	0.0	0.0	2.6734036438491663E-4	0.0
21	0.0	0.0	0.0	3.5645381917988886E-4	0.0
22	0.0	0.0	0.0	9.802480027446944E-4	0.0
23	0.0	0.0	0.0	0.002138722915079333	0.0
24	0.0	0.0	0.0	0.0034754247370039166	0.0
25	0.0	0.0	0.0	0.004099218920568722	0.0
26	0.0	0.0	0.0	0.005703261106878222	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACC	85	1.0581061E-8	20.703114	4
ATAGGCG	40	0.004488042	19.996437	5
CTAAGAC	65	9.433485E-6	19.692308	3
TACTAGA	60	1.1354314E-4	18.672491	2
ACACCGT	80	2.8008253E-6	17.996792	6
ATACTAG	45	0.008800272	17.795225	1
GTACCTA	55	0.0013662492	17.451435	6
TTAATAC	75	3.2335796E-5	17.066668	3
ATCGTTT	400	0.0	16.39927	29
GTCTAGG	130	4.7384674E-9	16.015703	1
ATAGATC	80	5.620667E-5	16.0	3
TACCGTC	270	0.0	15.997861	7
ATACCGT	280	0.0	15.425821	6
GTATTAG	365	0.0	15.357523	1
CGCTTCG	365	0.0	15.341783	32
GATATAC	115	3.307523E-7	15.319367	1
ACGCTTC	380	0.0	15.157219	31
GTCCTAC	85	9.353312E-5	15.073603	1
GTCTAGA	85	9.353312E-5	15.073603	1
CCGTGTG	85	9.435454E-5	15.058151	9
>>END_MODULE