FastQCFastQC Report
Thu 2 Feb 2017
SRR4062878_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062878_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1122165
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT44660.3979806891143459No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT34170.3045006750344201No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG19650.1751079386721204No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT18800.16753329501454778No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA17780.1584437226254606No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC17470.15568120552681647No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA17340.15452273061448182No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC17310.1542553902500969No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT17170.1530078018829673No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC16760.14935415023637344No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA16690.14873035605280863No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG16550.14748276768567903No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG16040.14293798149113546No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15180.13527422437876782No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG14890.13268993418971364No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG14650.13055121127463432No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG14410.12841248835955496No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG14150.12609553853488567No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT13980.12458060980337117No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA13660.12172897924993206No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT13440.11976848324444266No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC13260.11816444105813316No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT13120.11691685269100355No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA12990.11575837777866892No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC12790.11397610868276947No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG12420.1106789108553555No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT12420.1106789108553555No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT11710.10435185556491246No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC11360.10123288464708843No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG11290.10060909046352363No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGCG350.002065322822.8594025
GTACCGT507.191009E-419.2018976
GTCTTAC550.001363307417.4570481
TACCGTC3900.016.8221767
AAGACGG5000.016.6416455
ACCGTCG4050.016.5942348
GTATTAG3350.016.2411351
AACCGCG2200.016.0015817
TACTGCG703.693519E-416.0015817
CTACACG703.6947726E-416.0008684
GTGACCG600.002440973216.0008689
GCGCAAG5250.015.8498921
CTAGATA3650.015.7816793
CAAGACG5400.015.7045564
ATACCGT4200.015.6205936
TCTAGAT3600.015.5557072
CGGCATC3300.015.51461125
GACGGAC5500.015.1287687
GTAACAC756.241615E-414.9341453
AACGCTT4750.014.82053830
Produced by FastQC (version 0.11.5)