##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062878_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1122165
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.189748388160385	32.0	32.0	32.0	32.0	32.0
2	31.310285029385163	32.0	32.0	32.0	32.0	32.0
3	31.38790819531887	32.0	32.0	32.0	32.0	32.0
4	31.48480749265928	32.0	32.0	32.0	32.0	32.0
5	31.414154781159635	32.0	32.0	32.0	32.0	32.0
6	34.93325045782037	36.0	36.0	36.0	36.0	36.0
7	34.95731019948047	36.0	36.0	36.0	36.0	36.0
8	34.90156706010257	36.0	36.0	36.0	36.0	36.0
9	35.0036295910138	36.0	36.0	36.0	36.0	36.0
10	34.85444297407244	36.0	36.0	36.0	32.0	36.0
11	35.01799022425401	36.0	36.0	36.0	36.0	36.0
12	34.92478467961485	36.0	36.0	36.0	32.0	36.0
13	34.967216942250026	36.0	36.0	36.0	36.0	36.0
14	34.90946875013924	36.0	36.0	36.0	32.0	36.0
15	34.885234346107744	36.0	36.0	36.0	32.0	36.0
16	34.89467591664327	36.0	36.0	36.0	32.0	36.0
17	34.85528153168206	36.0	36.0	36.0	32.0	36.0
18	34.84866931333627	36.0	36.0	36.0	32.0	36.0
19	34.854611398502	36.0	36.0	36.0	32.0	36.0
20	34.82551763778054	36.0	36.0	36.0	32.0	36.0
21	34.81936435372695	36.0	36.0	36.0	32.0	36.0
22	34.80596703693307	36.0	36.0	36.0	32.0	36.0
23	34.73937076989569	36.0	36.0	36.0	32.0	36.0
24	34.72274487263459	36.0	36.0	36.0	32.0	36.0
25	34.70079355531495	36.0	36.0	36.0	32.0	36.0
26	34.641792428029746	36.0	36.0	36.0	32.0	36.0
27	34.620155681205524	36.0	36.0	36.0	32.0	36.0
28	34.58337766727709	36.0	36.0	36.0	32.0	36.0
29	34.5527110540785	36.0	36.0	36.0	32.0	36.0
30	34.5186340689649	36.0	36.0	36.0	32.0	36.0
31	34.518259792454764	36.0	36.0	36.0	32.0	36.0
32	34.47933414426577	36.0	36.0	36.0	32.0	36.0
33	34.43358507884313	36.0	36.0	36.0	32.0	36.0
34	34.43343091256634	36.0	36.0	36.0	32.0	36.0
35	34.37825720816458	36.0	36.0	36.0	32.0	36.0
36	34.34976852780117	36.0	36.0	36.0	32.0	36.0
37	34.34325789879385	36.0	36.0	36.0	32.0	36.0
38	33.90187539265616	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	5.0
21	31.0
22	91.0
23	267.0
24	706.0
25	1811.0
26	3931.0
27	7509.0
28	13285.0
29	21315.0
30	31575.0
31	46050.0
32	65935.0
33	104086.0
34	250714.0
35	574850.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.222552663967484	18.398099222238955	12.029406443643738	26.349941670149825
2	15.854442082937892	21.09217450196718	36.177656583479255	26.87572683161567
3	17.54180534948069	25.11243890158756	29.054194347533564	28.29156140139819
4	12.035071585029613	16.124320730874864	35.877974837857636	35.96263284623789
5	14.261373560370854	36.80495898995156	32.94580827757797	15.987859172099622
6	33.96684088347079	35.99675627024546	16.525109943724853	13.511292902558894
7	29.785538986349565	30.871567563328643	21.1938205000704	18.14907295025139
8	28.430587983674048	32.14064198763077	19.67570891332632	19.75306111536885
9	27.826653429840963	13.674092478357968	18.63914975377009	39.860104338030986
10	16.090796564117145	26.325015127116796	30.922054578815622	26.662133729950444
11	37.87780353280558	20.99863560735079	22.036322609795285	19.087238250048348
12	24.975538291262758	23.847893619637805	28.217583557454272	22.958984531645164
13	29.61265842186779	18.999395809161243	25.33074547168769	26.057200297283277
14	24.04129517495199	19.739521371634297	24.93349908435925	31.28568436905446
15	25.464080594208514	26.63164507893224	22.533406406366264	25.370867920492973
16	26.03104359077639	25.391475755771886	23.54602357587661	25.031457077575116
17	24.134418735212737	25.630188073946346	25.088556495702502	25.146836695138415
18	25.398225751115035	23.858256138803117	26.45956699772315	24.283951112358697
19	25.916420490747793	24.65742560140443	25.569234470866586	23.85691943698119
20	25.977195866917967	23.40039120806655	25.21358267277985	25.40883025223563
21	27.66852943823669	23.723202422464475	24.279004030608746	24.329264108690094
22	26.07542213081154	23.74848952559519	25.004277449641943	25.17181089395133
23	24.268556111227948	23.40424394295308	25.778050267162378	26.549149678656597
24	24.951678229137425	24.845098537202638	25.24789135287591	24.95533188078402
25	25.07322898147777	23.909139921491047	25.390651107457458	25.626979989573726
26	24.84717028883479	24.967206219411782	25.84016240050474	24.345461091248694
27	25.70811905222325	24.288093619197923	24.719670849956735	25.28411647862209
28	24.524022759576354	24.049939180067103	26.129847214981755	25.296190845374788
29	24.4931003907625	24.461554227765077	25.977195866917967	25.068149514554456
30	24.54398417345043	24.612512420187763	26.239902331653546	24.603601074708266
31	25.163679138094665	24.498981878778967	24.6916451680457	25.645693815080673
32	24.7288054786952	24.524557440305124	24.731211541974666	26.01542553902501
33	24.367183079137202	23.915377863326697	25.5263708991102	26.1910681584259
34	25.263664434374622	24.116952498072923	25.659060833299918	24.960322234252537
35	26.36385914727335	23.992995682453117	25.451159143263247	24.191986027010287
36	24.76302504533647	25.076793519669565	24.796086137065405	25.364095297928557
37	25.88104244919419	24.805443049818876	24.907032388285145	24.406482112701784
38	24.832644782759026	24.27996264360646	25.579416199414702	25.30797637421981
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	376.0
1	289.5
2	203.0
3	203.0
4	682.5
5	1162.0
6	1162.0
7	1384.5
8	1607.0
9	1582.0
10	1557.0
11	1557.0
12	1886.5
13	2216.0
14	2826.5
15	3437.0
16	3437.0
17	5130.5
18	6824.0
19	6824.0
20	7540.0
21	8256.0
22	8248.5
23	8241.0
24	8241.0
25	8976.0
26	9711.0
27	9711.0
28	12614.5
29	15518.0
30	19019.5
31	22521.0
32	22521.0
33	30027.0
34	37533.0
35	37533.0
36	41142.0
37	44751.0
38	51607.5
39	58464.0
40	58464.0
41	62337.0
42	66210.0
43	76334.0
44	86458.0
45	86458.0
46	89531.5
47	92605.0
48	92605.0
49	99051.5
50	105498.0
51	105329.5
52	105161.0
53	105161.0
54	99909.5
55	94658.0
56	94658.0
57	92912.0
58	91166.0
59	81398.0
60	71630.0
61	71630.0
62	69627.0
63	67624.0
64	55791.5
65	43959.0
66	43959.0
67	36584.0
68	29209.0
69	29209.0
70	23674.0
71	18139.0
72	14132.5
73	10126.0
74	10126.0
75	7521.5
76	4917.0
77	4917.0
78	5104.0
79	5291.0
80	4126.0
81	2961.0
82	2961.0
83	2862.0
84	2763.0
85	2763.0
86	1747.0
87	731.0
88	633.5
89	536.0
90	536.0
91	311.5
92	87.0
93	54.5
94	22.0
95	22.0
96	19.0
97	16.0
98	16.0
99	18.5
100	21.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008733118569907277
2	0.0
3	0.0
4	2.6734036438491663E-4
5	8.911345479497223E-5
6	0.0
7	2.6734036438491663E-4
8	0.002227836369874305
9	0.0038318785561838055
10	7.129076383597778E-4
11	0.0048121265589285
12	2.6734036438491663E-4
13	2.6734036438491663E-4
14	0.0
15	0.0
16	8.911345479497223E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	1.7822690958994446E-4
22	8.911345479497223E-5
23	8.911345479497223E-5
24	0.0
25	0.0
26	8.911345479497223E-5
27	1.7822690958994446E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	8.911345479497223E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1122165.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.87296014405723
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.48791750851007	37.870639040394074
2	13.588159201581758	13.281871261800196
3	4.094488643796377	6.003293408956659
4	1.6663097023748514	3.25749950687288
5	0.858191557614758	2.0971180895636232
6	0.505868738494139	1.4833981616729142
7	0.3384379127689826	1.1578323835397484
8	0.22866312675589962	0.894035510428527
9	0.18056415854428848	0.7942234431582217
>10	0.8206705194746555	7.406102823423136
>50	0.09431267899985289	3.2895538687765633
>100	0.11371072784886525	12.12931701519693
>500	0.01684603336836194	5.70503959066141
>1k	0.005859489867256327	4.630075895555138
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4466	0.3979806891143459	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3417	0.3045006750344201	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1965	0.1751079386721204	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1880	0.16753329501454778	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1778	0.1584437226254606	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1747	0.15568120552681647	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1734	0.15452273061448182	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1731	0.1542553902500969	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1717	0.1530078018829673	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1676	0.14935415023637344	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1669	0.14873035605280863	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1655	0.14748276768567903	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1604	0.14293798149113546	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1518	0.13527422437876782	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1489	0.13268993418971364	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1465	0.13055121127463432	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1441	0.12841248835955496	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1415	0.12609553853488567	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1398	0.12458060980337117	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1366	0.12172897924993206	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1344	0.11976848324444266	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1326	0.11816444105813316	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1312	0.11691685269100355	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1299	0.11575837777866892	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1279	0.11397610868276947	No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG	1242	0.1106789108553555	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1242	0.1106789108553555	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT	1171	0.10435185556491246	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	1136	0.10123288464708843	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	1129	0.10060909046352363	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	8.911345479497222E-5	0.0
6	0.0	0.0	0.0	8.911345479497222E-5	0.0
7	0.0	0.0	0.0	8.911345479497222E-5	0.0
8	0.0	0.0	0.0	8.911345479497222E-5	0.0
9	0.0	0.0	0.0	1.7822690958994443E-4	0.0
10	0.0	0.0	0.0	1.7822690958994443E-4	0.0
11	0.0	0.0	0.0	1.7822690958994443E-4	0.0
12	0.0	0.0	0.0	1.7822690958994443E-4	0.0
13	0.0	0.0	0.0	1.7822690958994443E-4	0.0
14	0.0	0.0	0.0	1.7822690958994443E-4	0.0
15	0.0	0.0	0.0	3.5645381917988886E-4	0.0
16	0.0	0.0	0.0	3.5645381917988886E-4	0.0
17	0.0	0.0	0.0	3.5645381917988886E-4	0.0
18	0.0	0.0	0.0	3.5645381917988886E-4	0.0
19	0.0	0.0	0.0	3.5645381917988886E-4	0.0
20	0.0	0.0	0.0	3.5645381917988886E-4	0.0
21	0.0	0.0	0.0	4.455672739748611E-4	0.0
22	0.0	0.0	0.0	0.0010693614575396665	0.0
23	0.0	0.0	0.0	0.0023169498246692777	0.0
24	0.0	0.0	0.0	0.003742765101388833	0.0
25	0.0	0.0	0.0	0.004277445830158666	0.0
26	0.0	0.0	0.0	0.005970601471263139	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGGCG	35	0.0020653228	22.859402	5
GTACCGT	50	7.191009E-4	19.201897	6
GTCTTAC	55	0.0013633074	17.457048	1
TACCGTC	390	0.0	16.822176	7
AAGACGG	500	0.0	16.641645	5
ACCGTCG	405	0.0	16.594234	8
GTATTAG	335	0.0	16.241135	1
AACCGCG	220	0.0	16.001581	7
TACTGCG	70	3.693519E-4	16.001581	7
CTACACG	70	3.6947726E-4	16.000868	4
GTGACCG	60	0.0024409732	16.000868	9
GCGCAAG	525	0.0	15.849892	1
CTAGATA	365	0.0	15.781679	3
CAAGACG	540	0.0	15.704556	4
ATACCGT	420	0.0	15.620593	6
TCTAGAT	360	0.0	15.555707	2
CGGCATC	330	0.0	15.514611	25
GACGGAC	550	0.0	15.128768	7
GTAACAC	75	6.241615E-4	14.934145	3
AACGCTT	475	0.0	14.820538	30
>>END_MODULE