FastQCFastQC Report
Thu 2 Feb 2017
SRR4062877_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062877_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2618384
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT64400.2459532291673032No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT44140.16857725986715472No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT41360.15796002419813138No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC41240.1575017262555836No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA38280.14619704367273859No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA36960.14115576630471313No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG36020.1375657657547556No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC35620.13603810594626306No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG35160.1342812971664966No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC34480.13168427549205924No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG34230.13072948811175136No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG30820.11770618824435224No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG30720.1173242732922291No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT30560.11671320936883207No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC30510.11652225189277052No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA29360.11213022994335438No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA29110.11117544256304653No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG28320.1081583144412737No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA27460.10487384585301468No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC27330.10437735641525461No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT27100.10349895202537136No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC26910.1027733136163374No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA26480.1011310793222079No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGGC7450.017.82481230
CTAGCGG7300.017.75273729
TCTAGCG7350.017.63129428
CGAATGT3300.016.96936223
GTCCTAC27100.016.728611
TGTAGGA27400.016.7086982
AGGACCT18000.016.5289045
GATATAC8050.016.516761
GTAGGAC27750.016.374143
AGCGGCG8400.015.80890931
TCCTACA29300.015.6798332
CTGTAGG28000.015.6759991
TAGGACC23350.015.6195374
ATCGTTT6400.015.49939929
TAGGACG24200.015.467524
GGACCTG18600.015.3080186
ATTTAGA8950.015.2138291
CGGTCCA8950.015.19523110
CCTACAG29800.015.1940413
TCTAGAT5800.015.1796542
Produced by FastQC (version 0.11.5)