##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062877_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2618384
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.246173594094678	32.0	32.0	32.0	32.0	32.0
2	30.863880546168936	32.0	32.0	32.0	32.0	32.0
3	30.916390032936345	32.0	32.0	32.0	32.0	32.0
4	30.977891325336543	32.0	32.0	32.0	32.0	32.0
5	30.900823179487805	32.0	32.0	32.0	32.0	32.0
6	34.57288121222861	36.0	36.0	36.0	32.0	36.0
7	34.48813428435248	36.0	36.0	36.0	32.0	36.0
8	34.47111233493636	36.0	36.0	36.0	32.0	36.0
9	34.605566257661216	36.0	36.0	36.0	32.0	36.0
10	34.32173966843671	36.0	36.0	36.0	32.0	36.0
11	34.59987763444934	36.0	36.0	36.0	32.0	36.0
12	34.40380287994427	36.0	36.0	36.0	32.0	36.0
13	34.49872440405991	36.0	36.0	36.0	32.0	36.0
14	34.39524340203729	36.0	36.0	36.0	32.0	36.0
15	34.33935816900806	36.0	36.0	36.0	32.0	36.0
16	34.360040391325334	36.0	36.0	36.0	32.0	36.0
17	34.280426782320696	36.0	36.0	36.0	32.0	36.0
18	34.32173890460681	36.0	36.0	36.0	32.0	36.0
19	34.28942317093291	36.0	36.0	36.0	32.0	36.0
20	34.26871536031384	36.0	36.0	36.0	32.0	36.0
21	34.24695881123624	36.0	36.0	36.0	32.0	36.0
22	34.21800049190646	36.0	36.0	36.0	32.0	36.0
23	34.17089166447702	36.0	36.0	36.0	32.0	36.0
24	34.15189979773784	36.0	36.0	36.0	32.0	36.0
25	34.11506257294575	36.0	36.0	36.0	32.0	36.0
26	34.077575328905155	36.0	36.0	36.0	32.0	36.0
27	34.07036095545955	36.0	36.0	36.0	32.0	36.0
28	34.04192700535903	36.0	36.0	36.0	32.0	36.0
29	33.995697728064336	36.0	36.0	36.0	32.0	36.0
30	33.95887959901985	36.0	36.0	36.0	32.0	36.0
31	33.97638314319061	36.0	36.0	36.0	32.0	36.0
32	33.93608080403791	36.0	36.0	36.0	32.0	36.0
33	33.904753466260104	36.0	36.0	36.0	32.0	36.0
34	33.90674171550086	36.0	36.0	36.0	32.0	36.0
35	33.86966197471418	36.0	36.0	36.0	32.0	36.0
36	33.807914347169856	36.0	36.0	36.0	32.0	36.0
37	33.797231040214115	36.0	36.0	36.0	32.0	36.0
38	33.18136491820909	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	3.0
7	4.0
8	11.0
9	10.0
10	28.0
11	25.0
12	13.0
13	16.0
14	237.0
15	522.0
16	771.0
17	946.0
18	1196.0
19	1682.0
20	2268.0
21	3331.0
22	5137.0
23	7696.0
24	11619.0
25	17552.0
26	25619.0
27	36701.0
28	51692.0
29	69822.0
30	93853.0
31	125738.0
32	169743.0
33	252908.0
34	560729.0
35	1178511.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.288954643782134	17.525225622433418	11.484307095066	25.70151263871846
2	15.874403065293393	19.65019062551229	39.239737232179465	25.23566907701485
3	19.085129642092944	24.132695505898656	28.62202231122811	28.16015254078029
4	12.00969327543339	15.443396694814593	36.75890173432481	35.788008295427204
5	13.8942887543357	37.254399479372346	34.13689696445662	14.714414801835336
6	33.79067788583458	36.21863322914662	17.104609880322766	12.886079004696041
7	29.784779220441944	30.56843140856888	21.205775630055363	18.441013740933805
8	27.502436632283928	34.519441117520536	19.51467478979273	18.463447460402815
9	27.434131906902635	14.136008680186238	18.87772463629187	39.552134776619255
10	15.835900773268225	26.662572511798384	31.96469402493997	25.53683268999342
11	37.71294657912456	20.734776217278174	23.048930975231837	18.50334622836543
12	25.225594765460503	23.515783708595762	29.465176081741557	21.793445444202174
13	29.83658631520686	19.601582455326806	25.45515453462375	25.106676694842584
14	22.903479622627096	20.95435621252053	25.002520912111837	31.13964325274054
15	24.352454720345705	29.281416126631754	22.364283280335805	24.00184587268674
16	24.77427856610836	25.73843127518573	25.807681289509365	23.67960886919654
17	23.143599530599644	26.717505638305024	26.214871373301634	23.924023457793698
18	24.36074695250041	24.517227787513004	28.4111065108555	22.710918749131086
19	25.441979401603792	25.02355657492939	25.538802940227217	23.995661083239597
20	25.4196666499886	24.72888909938053	25.94552230169555	23.905921948935323
21	26.45053853792682	24.5608815216561	24.296615995722252	24.691963944694827
22	25.35134286594945	25.67022984383361	25.328693449506122	23.64973384071082
23	23.133658506774022	25.18465751750322	26.46150437914663	25.22017959657613
24	24.629598788680703	25.520708395658932	26.17063895259384	23.679053863066528
25	24.249377553101844	25.30851134537915	26.205682888611992	24.236428212907015
26	23.093989427149207	26.657809481644577	26.994270460689496	23.25393063051672
27	24.36163089732048	25.72490075882781	25.855872751294157	24.05759559255755
28	23.218939919395496	25.796534739685345	26.187335193123868	24.79719014779529
29	23.59260614514684	25.463605577552283	26.02764788970179	24.91614038759909
30	23.263792743248523	25.877303373704635	26.86191960444246	23.99698427860438
31	24.794859376885835	25.323389621282377	25.212091953109844	24.669659048721947
32	23.69447678238046	26.099922694508866	25.32522542342326	24.880375099687416
33	23.09039123561336	25.637351082313746	26.617970537110107	24.65428714496278
34	24.217860130404393	25.91720428875367	26.63351171309506	23.231423867746877
35	24.782401426490146	25.703829108436015	26.588815284346882	22.92495418072695
36	23.116983720875155	26.381326791838205	26.314251510912722	24.187437976373914
37	24.46885226866465	26.195903600468124	25.325617316959143	24.00962681390808
38	23.644632325784002	26.4244036267651	25.70998177756406	24.220982269886843
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	175.0
1	259.5
2	344.0
3	344.0
4	881.5
5	1419.0
6	1419.0
7	1763.5
8	2108.0
9	2152.0
10	2196.0
11	2196.0
12	2896.5
13	3597.0
14	4671.0
15	5745.0
16	5745.0
17	8496.0
18	11247.0
19	11247.0
20	13671.0
21	16095.0
22	18363.0
23	20631.0
24	20631.0
25	26973.5
26	33316.0
27	33316.0
28	43771.5
29	54227.0
30	69269.0
31	84311.0
32	84311.0
33	106440.5
34	128570.0
35	128570.0
36	138123.0
37	147676.0
38	162227.0
39	176778.0
40	176778.0
41	175839.0
42	174900.0
43	189045.5
44	203191.0
45	203191.0
46	206312.5
47	209434.0
48	209434.0
49	217706.0
50	225978.0
51	225324.5
52	224671.0
53	224671.0
54	209893.0
55	195115.0
56	195115.0
57	188919.5
58	182724.0
59	163150.5
60	143577.0
61	143577.0
62	137968.5
63	132360.0
64	109231.5
65	86103.0
66	86103.0
67	72289.0
68	58475.0
69	58475.0
70	46963.0
71	35451.0
72	27377.5
73	19304.0
74	19304.0
75	14584.5
76	9865.0
77	9865.0
78	9951.0
79	10037.0
80	8064.0
81	6091.0
82	6091.0
83	6153.0
84	6215.0
85	6215.0
86	4120.5
87	2026.0
88	1743.0
89	1460.0
90	1460.0
91	1076.5
92	693.0
93	626.5
94	560.0
95	560.0
96	505.5
97	451.0
98	451.0
99	859.5
100	1268.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.125459061772452
2	0.05625607244773876
3	0.012603193420063672
4	0.0028261706457112477
5	2.2914897127388495E-4
6	3.055319616985133E-4
7	1.1457448563694248E-4
8	6.110639233970266E-4
9	2.6734046648619914E-4
10	6.492554186093407E-4
11	0.002406064198375792
12	0.0031698941026220753
13	0.016307768455658147
14	0.010846384640297222
15	0.02490085487842883
16	0.012755959400912929
17	0.021998301242292954
18	0.006568937176518036
19	0.0083639374514968
20	0.006683511662154979
21	0.007027235119065805
22	0.008211171470647543
23	0.011228299592420363
24	0.015734896027473435
25	0.018713832654033938
26	0.020890747881135846
27	0.01019712922168788
28	0.006416171195668779
29	0.012144895477515903
30	0.004010106997292987
31	0.006874469138216549
32	0.007638299042462833
33	0.009280533336592341
34	0.014283619209405496
35	0.018713832654033938
36	0.016422342941295087
37	0.010693618659447965
38	0.006989043623853492
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2618384.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.02160078753727
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.616426307323	33.14329356613452
2	14.570277960171863	13.11954475372621
3	5.332476266022228	7.202298529736102
4	2.35885436337453	4.247975978551538
5	1.2436614287534709	2.799581418009849
6	0.7317912619873597	1.97678484342018
7	0.44548779563016466	1.4039601583406884
8	0.2993324959450641	1.0781142508140635
9	0.21539712903017091	0.8727771198580209
>10	0.9851614806471237	7.8106857394564635
>50	0.08063913420923782	2.561686361810391
>100	0.09197848060315295	9.017436140454368
>500	0.01745002609566851	5.5095141173850415
>1k	0.010980748128493497	9.009544826231679
>5k	8.512207851545347E-5	0.2468021960710528
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	6440	0.2459532291673032	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	4414	0.16857725986715472	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	4136	0.15796002419813138	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	4124	0.1575017262555836	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	3828	0.14619704367273859	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	3696	0.14115576630471313	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	3602	0.1375657657547556	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	3562	0.13603810594626306	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	3516	0.1342812971664966	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	3448	0.13168427549205924	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3423	0.13072948811175136	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	3082	0.11770618824435224	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	3072	0.1173242732922291	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	3056	0.11671320936883207	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	3051	0.11652225189277052	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2936	0.11213022994335438	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	2911	0.11117544256304653	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2832	0.1081583144412737	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	2746	0.10487384585301468	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2733	0.10437735641525461	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	2710	0.10349895202537136	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	2691	0.1027733136163374	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA	2648	0.1011310793222079	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5276598084925665E-4
2	0.0	0.0	0.0	0.0	1.909574760615708E-4
3	0.0	0.0	0.0	0.0	1.909574760615708E-4
4	0.0	0.0	0.0	0.0	1.909574760615708E-4
5	0.0	0.0	0.0	3.819149521231416E-5	1.909574760615708E-4
6	0.0	0.0	0.0	3.819149521231416E-5	1.909574760615708E-4
7	0.0	0.0	0.0	1.5276598084925665E-4	1.909574760615708E-4
8	0.0	0.0	0.0	1.5276598084925665E-4	1.909574760615708E-4
9	0.0	0.0	0.0	1.5276598084925665E-4	1.909574760615708E-4
10	0.0	0.0	0.0	1.5276598084925665E-4	1.909574760615708E-4
11	0.0	0.0	0.0	1.5276598084925665E-4	1.909574760615708E-4
12	0.0	0.0	0.0	2.2914897127388495E-4	6.110639233970266E-4
13	0.0	0.0	0.0	2.2914897127388495E-4	6.874469138216549E-4
14	0.0	0.0	0.0	2.2914897127388495E-4	6.874469138216549E-4
15	0.0	0.0	0.0	2.2914897127388495E-4	9.165958850955398E-4
16	0.0	0.0	0.0	2.6734046648619914E-4	9.165958850955398E-4
17	0.0	0.0	0.0	2.6734046648619914E-4	9.165958850955398E-4
18	0.0	0.0	0.0	3.055319616985133E-4	0.0010693618659447966
19	0.0	0.0	0.0	3.819149521231416E-4	0.0011075533611571106
20	0.0	0.0	0.0	4.964894377600841E-4	0.0013367023324309957
21	0.0	0.0	0.0	5.346809329723983E-4	0.001451276818067938
22	0.0	0.0	0.0	8.402128946709115E-4	0.0014894683132802523
23	0.0	0.0	0.0	0.0014894683132802523	0.0014894683132802523
24	0.0	0.0	0.0	0.002367872703163478	0.0015276598084925663
25	0.0	0.0	0.0	0.0027497876552866197	0.0015276598084925663
26	0.0	0.0	0.0	0.003131702607409761	0.0016040427989171947
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGGC	745	0.0	17.824812	30
CTAGCGG	730	0.0	17.752737	29
TCTAGCG	735	0.0	17.631294	28
CGAATGT	330	0.0	16.969362	23
GTCCTAC	2710	0.0	16.72861	1
TGTAGGA	2740	0.0	16.708698	2
AGGACCT	1800	0.0	16.528904	5
GATATAC	805	0.0	16.51676	1
GTAGGAC	2775	0.0	16.37414	3
AGCGGCG	840	0.0	15.808909	31
TCCTACA	2930	0.0	15.679833	2
CTGTAGG	2800	0.0	15.675999	1
TAGGACC	2335	0.0	15.619537	4
ATCGTTT	640	0.0	15.499399	29
TAGGACG	2420	0.0	15.46752	4
GGACCTG	1860	0.0	15.308018	6
ATTTAGA	895	0.0	15.213829	1
CGGTCCA	895	0.0	15.195231	10
CCTACAG	2980	0.0	15.194041	3
TCTAGAT	580	0.0	15.179654	2
>>END_MODULE