FastQCFastQC Report
Thu 2 Feb 2017
SRR4062877_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062877_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2618384
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT73220.2796381279445643No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT53950.20604311667043487No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC43050.16441438688901247No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT41350.15792183270291904No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA38520.14711363955783413No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA38450.14684629909134794No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC38040.14528044778764307No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG37320.14253066013235643No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC36760.14039193640046685No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC35530.13569438248935223No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG33950.12966012624580658No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA32190.12293842308843927No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG32140.12274746561237772No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG32070.1224801251458915No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC31990.122174593184193No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT31230.11927203954805712No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG30690.11720969880659214No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA30380.11602576245501042No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT29690.11339054928536074No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG29650.11323778330451148No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA29410.11232118741941594No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG27710.10582863323332255No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT26770.10223863268336501No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA26520.10128384530305715No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT26350.1006345898844478No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG8400.018.47560728
CTAGCGG8400.018.47560729
TAGCGGC8750.017.73658430
ATCGTTT6800.016.70535529
AAGACGG9250.016.60715
GTCCTAC24400.016.4597531
TCCTACA26050.016.2764992
CAAGACG9350.016.2571014
GCGCAAG9100.016.1765481
TGTAGGA26850.015.8511292
CGCAAGA9250.015.7406442
CATCGTT7150.015.66383928
GTAGGAC27300.015.5901443
AGCGGCG10200.015.5289231
GTATTAG7200.015.3340191
TAGGACA9300.015.3122224
GATATAC8500.015.2477421
CGCTTCG6550.015.1445632
TAAACGC6450.015.13130528
TAGGACG28150.015.0625544
Produced by FastQC (version 0.11.5)