##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062877_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2618384
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.224459055661814	32.0	32.0	32.0	32.0	32.0
2	31.32721060012588	32.0	32.0	32.0	32.0	32.0
3	31.413796066581526	32.0	32.0	32.0	32.0	32.0
4	31.505836424298344	32.0	32.0	32.0	32.0	32.0
5	31.441189680352462	32.0	32.0	32.0	32.0	32.0
6	34.976330438927214	36.0	36.0	36.0	36.0	36.0
7	34.999414906293346	36.0	36.0	36.0	36.0	36.0
8	34.94159603786152	36.0	36.0	36.0	36.0	36.0
9	35.04299483956517	36.0	36.0	36.0	36.0	36.0
10	34.89568718721166	36.0	36.0	36.0	32.0	36.0
11	35.071956214214566	36.0	36.0	36.0	36.0	36.0
12	34.96381508594614	36.0	36.0	36.0	36.0	36.0
13	35.014335941557846	36.0	36.0	36.0	36.0	36.0
14	34.95903465649042	36.0	36.0	36.0	32.0	36.0
15	34.93390274306595	36.0	36.0	36.0	36.0	36.0
16	34.94350141155766	36.0	36.0	36.0	36.0	36.0
17	34.91289054623004	36.0	36.0	36.0	32.0	36.0
18	34.91581868816797	36.0	36.0	36.0	32.0	36.0
19	34.90398047039701	36.0	36.0	36.0	32.0	36.0
20	34.88890896064137	36.0	36.0	36.0	32.0	36.0
21	34.87068703444567	36.0	36.0	36.0	32.0	36.0
22	34.85561285128537	36.0	36.0	36.0	32.0	36.0
23	34.79839473507324	36.0	36.0	36.0	32.0	36.0
24	34.776036669946045	36.0	36.0	36.0	32.0	36.0
25	34.750948676741075	36.0	36.0	36.0	32.0	36.0
26	34.68035475316073	36.0	36.0	36.0	32.0	36.0
27	34.660657107590026	36.0	36.0	36.0	32.0	36.0
28	34.624908722326445	36.0	36.0	36.0	32.0	36.0
29	34.58673212179726	36.0	36.0	36.0	32.0	36.0
30	34.55140307915111	36.0	36.0	36.0	32.0	36.0
31	34.55643251715562	36.0	36.0	36.0	32.0	36.0
32	34.526251306149135	36.0	36.0	36.0	32.0	36.0
33	34.49575998020153	36.0	36.0	36.0	32.0	36.0
34	34.48004570758147	36.0	36.0	36.0	32.0	36.0
35	34.44143716124144	36.0	36.0	36.0	32.0	36.0
36	34.40229087864881	36.0	36.0	36.0	32.0	36.0
37	34.386120981490876	36.0	36.0	36.0	32.0	36.0
38	33.94848692934268	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	12.0
21	50.0
22	179.0
23	564.0
24	1612.0
25	3935.0
26	8466.0
27	16644.0
28	28774.0
29	46038.0
30	70914.0
31	102516.0
32	148596.0
33	237531.0
34	585175.0
35	1367378.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.79792417597066	18.61973820355741	12.130591897075062	26.45174572339687
2	14.962058644590956	20.696322883245116	38.422988539109824	25.918629933054106
3	18.215548215998876	24.742512939278576	29.01346784887167	28.028470995850874
4	11.567071407866159	16.256113987994873	36.88198929032864	35.29482531381033
5	13.379827695118587	37.76064760604068	33.859994454590655	14.999530244250076
6	33.893756404248585	36.51304872212923	16.70247126179976	12.89072361182243
7	29.541364571016537	30.77655768308295	21.180647851950983	18.50142989394953
8	27.32880445264822	34.712048663506614	19.340512781732624	18.618634102112548
9	27.18947673161785	13.94109249070189	18.792838335483605	40.07659244219665
10	15.68591580709816	26.946250488854123	31.857744977023856	25.510088727023856
11	37.71681063737066	20.722042012175347	23.020837272046386	18.5403100784076
12	25.0031890020295	23.687105050691766	29.410046082034118	21.899659865244615
13	29.79132135136993	19.563623309068966	25.468839511453645	25.176215828107456
14	22.8265220074672	20.923401609542374	25.014169044723765	31.235907338266657
15	24.344366601690204	29.368648754346193	22.24459819491717	24.042386449046436
16	24.80229210165205	25.849656066358513	25.59743933565105	23.750612496338388
17	23.116242690147818	26.914654229478945	25.966512169338035	24.002590911035206
18	24.408375547666044	24.55377056993932	28.37891615591907	22.658937726475568
19	25.464103049820043	25.002253298217525	25.607779454808764	23.925864197153665
20	25.485108372186815	24.717039211971965	25.86675598384347	23.93109643199775
21	26.416820164827044	24.53966783290896	24.383960928527973	24.659551073736022
22	25.32161107249521	25.652426309397413	25.40429578594398	23.6216668321634
23	23.114800241217576	25.161750591872924	26.447124045641914	25.27632512126759
24	24.581421212473035	25.50882529071366	26.12886421548558	23.78088928132772
25	24.260735363367946	25.348774948164493	26.142242967882034	24.248246720585524
26	23.1455238943454	26.668273257406007	26.91785496457544	23.268347883673155
27	24.453842105544567	25.656836932120676	25.800093340085596	24.089227622249158
28	23.282757609273506	25.66048371820176	26.276970833918938	24.779787838605795
29	23.607232552597328	25.294036321639606	26.191116352681654	24.907614773081413
30	23.288944631497902	25.674423613954257	26.900981674192938	24.135650080354907
31	24.7072239977024	25.177514069746838	25.333640902174775	24.781621030375987
32	23.75262757487061	25.931948866170888	25.421099426210976	24.894324132747528
33	23.144237056138444	25.447986238840446	26.706357814590987	24.701418890430126
34	24.21757083758532	25.751952349235253	26.76521090871316	23.26526590446627
35	24.83680774095778	25.603005517907228	26.555081302055004	23.00510543907998
36	23.185101956015618	26.239657743096505	26.372373188959298	24.202867111928576
37	24.591589542095253	25.977826773241347	25.35049303329574	24.08009065136766
38	23.749808564764688	26.06872419920875	25.839651173493216	24.341816062533347
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	84.0
1	131.5
2	179.0
3	179.0
4	693.5
5	1208.0
6	1208.0
7	1668.5
8	2129.0
9	2273.0
10	2417.0
11	2417.0
12	3023.0
13	3629.0
14	4682.5
15	5736.0
16	5736.0
17	8914.0
18	12092.0
19	12092.0
20	14275.0
21	16458.0
22	18519.0
23	20580.0
24	20580.0
25	26560.0
26	32540.0
27	32540.0
28	43058.0
29	53576.0
30	68256.5
31	82937.0
32	82937.0
33	105905.5
34	128874.0
35	128874.0
36	138372.0
37	147870.0
38	162626.5
39	177383.0
40	177383.0
41	176616.5
42	175850.0
43	189447.0
44	203044.0
45	203044.0
46	206862.0
47	210680.0
48	210680.0
49	219143.0
50	227606.0
51	225285.5
52	222965.0
53	222965.0
54	209307.0
55	195649.0
56	195649.0
57	190016.5
58	184384.0
59	164143.5
60	143903.0
61	143903.0
62	138430.5
63	132958.0
64	109744.0
65	86530.0
66	86530.0
67	72581.5
68	58633.0
69	58633.0
70	47106.5
71	35580.0
72	27611.0
73	19642.0
74	19642.0
75	14504.0
76	9366.0
77	9366.0
78	9731.5
79	10097.0
80	7916.0
81	5735.0
82	5735.0
83	5554.5
84	5374.0
85	5374.0
86	3368.0
87	1362.0
88	1189.5
89	1017.0
90	1017.0
91	603.5
92	190.0
93	131.5
94	73.0
95	73.0
96	49.0
97	25.0
98	25.0
99	27.0
100	29.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009280533336592341
2	3.819149521231416E-5
3	0.0
4	1.1457448563694248E-4
5	7.638299042462833E-5
6	1.9095747606157081E-4
7	3.055319616985133E-4
8	0.0029407451313481904
9	0.004201064473354558
10	6.110639233970266E-4
11	0.005461383815360925
12	3.8191495212314163E-4
13	1.5276598084925665E-4
14	0.0
15	0.0
16	3.819149521231416E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	1.1457448563694248E-4
22	1.5276598084925665E-4
23	3.819149521231416E-5
24	0.0
25	1.9095747606157081E-4
26	1.1457448563694248E-4
27	7.638299042462833E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	3.819149521231416E-5
38	1.9095747606157081E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2618384.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.61549328931076
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.21229595460153	30.34747120369734
2	15.995551847040959	13.63316664792793
3	5.65680731334323	7.232029023021074
4	2.6243196599746392	4.473467074346222
5	1.317407906417586	2.807099389761179
6	0.7964951397594051	2.0365819970031342
7	0.5072781192627954	1.513253510108049
8	0.3456658728418789	1.1784577347549472
9	0.24197781354405806	0.9280803500324009
>10	1.0860226962220392	8.152518898094119
>50	0.08577366869474246	2.580110860113939
>100	0.09758769876996341	8.993367062136167
>500	0.019834033230772895	5.795752443716542
>1k	0.012801966903498607	9.84005833581178
>5k	1.8030939300702265E-4	0.4885854694751986
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	7322	0.2796381279445643	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	5395	0.20604311667043487	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	4305	0.16441438688901247	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	4135	0.15792183270291904	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	3852	0.14711363955783413	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	3845	0.14684629909134794	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	3804	0.14528044778764307	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3732	0.14253066013235643	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	3676	0.14039193640046685	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	3553	0.13569438248935223	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	3395	0.12966012624580658	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	3219	0.12293842308843927	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	3214	0.12274746561237772	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	3207	0.1224801251458915	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	3199	0.122174593184193	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	3123	0.11927203954805712	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	3069	0.11720969880659214	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	3038	0.11602576245501042	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2969	0.11339054928536074	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2965	0.11323778330451148	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	2941	0.11232118741941594	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	2771	0.10582863323332255	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	2677	0.10223863268336501	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	2652	0.10128384530305715	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	2635	0.1006345898844478	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.909574760615708E-4
2	0.0	0.0	0.0	0.0	1.909574760615708E-4
3	0.0	0.0	0.0	3.819149521231416E-5	1.909574760615708E-4
4	0.0	0.0	0.0	3.819149521231416E-5	1.909574760615708E-4
5	0.0	0.0	0.0	7.638299042462833E-5	2.2914897127388495E-4
6	0.0	0.0	0.0	7.638299042462833E-5	3.055319616985133E-4
7	0.0	0.0	0.0	1.5276598084925665E-4	3.055319616985133E-4
8	0.0	0.0	0.0	1.5276598084925665E-4	3.4372345691082747E-4
9	0.0	0.0	0.0	1.5276598084925665E-4	3.4372345691082747E-4
10	0.0	0.0	0.0	1.5276598084925665E-4	3.4372345691082747E-4
11	0.0	0.0	0.0	1.909574760615708E-4	3.4372345691082747E-4
12	0.0	0.0	0.0	2.6734046648619914E-4	6.874469138216549E-4
13	0.0	0.0	0.0	2.6734046648619914E-4	9.165958850955398E-4
14	0.0	0.0	0.0	2.6734046648619914E-4	9.165958850955398E-4
15	0.0	0.0	0.0	2.6734046648619914E-4	0.0011075533611571106
16	0.0	0.0	0.0	3.4372345691082747E-4	0.0011075533611571106
17	0.0	0.0	0.0	3.4372345691082747E-4	0.0011457448563694248
18	0.0	0.0	0.0	3.4372345691082747E-4	0.0012221278467940532
19	0.0	0.0	0.0	3.4372345691082747E-4	0.0012985108372186814
20	0.0	0.0	0.0	4.2010644733545574E-4	0.001451276818067938
21	0.0	0.0	0.0	4.582979425477699E-4	0.0015276598084925663
22	0.0	0.0	0.0	7.25638409033969E-4	0.0015276598084925663
23	0.0	0.0	0.0	0.0013367023324309957	0.0015276598084925663
24	0.0	0.0	0.0	0.0022532982175265353	0.0015276598084925663
25	0.0	0.0	0.0	0.0025206386840127344	0.0015276598084925663
26	0.0	0.0	0.0	0.0028261706457112477	0.0015276598084925663
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	840	0.0	18.475607	28
CTAGCGG	840	0.0	18.475607	29
TAGCGGC	875	0.0	17.736584	30
ATCGTTT	680	0.0	16.705355	29
AAGACGG	925	0.0	16.6071	5
GTCCTAC	2440	0.0	16.459753	1
TCCTACA	2605	0.0	16.276499	2
CAAGACG	935	0.0	16.257101	4
GCGCAAG	910	0.0	16.176548	1
TGTAGGA	2685	0.0	15.851129	2
CGCAAGA	925	0.0	15.740644	2
CATCGTT	715	0.0	15.663839	28
GTAGGAC	2730	0.0	15.590144	3
AGCGGCG	1020	0.0	15.52892	31
GTATTAG	720	0.0	15.334019	1
TAGGACA	930	0.0	15.312222	4
GATATAC	850	0.0	15.247742	1
CGCTTCG	655	0.0	15.14456	32
TAAACGC	645	0.0	15.131305	28
TAGGACG	2815	0.0	15.062554	4
>>END_MODULE