FastQCFastQC Report
Thu 2 Feb 2017
SRR4062876_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062876_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4597
Sequences flagged as poor quality0
Sequence length38
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4289.310419839025451No Hit
CTATTAAGACTCGCTTTCGCTACGGCTCCGCTTTTTCG270.5873395692843159No Hit
GTATCAACGCAGAGTACATGGGTTCGGACCTCCGGTGC210.45681966499891236No Hit
ATTAAGACTCGCTTTCGCTACGGCTCCGCTTTTTCGGC190.41331303023711113No Hit
AGTTACAGTTGCATGCAAGGTTAAGCCGAAAAAGCGGA180.3915597128562106No Hit
ATACTATGACGCCCTATTAAGACTCGCTTTCGCTACGG180.3915597128562106No Hit
GTTAATGGGTGATGGCGTGCCTCTTGTAGAATGAACCG170.36980639547531No Hit
TATCAACGCAGAGTACATGGGCCGGAAGGGGAGTGAAA170.36980639547531No Hit
CTCTTGTAGAATGAACCGGCGAGTTACAGTTGCATGCA160.3480530780944094No Hit
TGCCTACACAGTCAGAGCCCGTTAATGGGTGATGGCGT150.3262997607135088No Hit
GTATCAACGCAGAGTACATGGGCCGGAAGGGGAGTGAA150.3262997607135088No Hit
TTACAGTTGCATGCAAGGTTAAGCCGAAAAAGCGGAGC140.3045464433326082No Hit
GTACATGGGTTCGGACCTCCGGTGCGTATTACCGCACT140.3045464433326082No Hit
GGCTCTGACTGTGTAGGCACATGGTTTCAGGAACTATT140.3045464433326082No Hit
GTCAGAGCCCGTTAATGGGTGATGGCGTGCCTCTTGTA130.28279312595170764No Hit
GTGTAGGCACATGGTTTCAGGAACTATTTCACTCCCCT130.28279312595170764No Hit
TTCTACAAGAGGCACGCCATCACCCATTAACGGGCTCT130.28279312595170764No Hit
TATCAACGCAGAGTACATGGGTTCGGACCTCCGGTGCG120.26103980857080705No Hit
GCGTATTACCGCACTTTCATCCTGGACATGGGTAGATC120.26103980857080705No Hit
ACGCAGAGTACATGGGCCGGAAGGGGAGTGAAATAGTT120.26103980857080705No Hit
CTTGTAGAATGAACCGGCGAGTTACAGTTGCATGCAAG110.23928649118990647No Hit
TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT110.23928649118990647No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT110.23928649118990647No Hit
TCATAGTATGTTGCCGTAGACCCGAAACCAGGTGATCT110.23928649118990647No Hit
CTACAAGAGGCACGCCATCACCCATTAACGGGCTCTGA100.21753317380900586No Hit
GTTGCATGCAAGGTTAAGCCGAAAAAGCGGAGCCGTAG100.21753317380900586No Hit
ACTGTAACTCGCCGGTTCATTCTACAAGAGGCACGCCA100.21753317380900586No Hit
CCGTTAATGGGTGATGGCGTGCCTCTTGTAGAATGAAC90.1957798564281053No Hit
GGACATGGGTAGATCACCTGGTTTCGGGTCTACGGCAA90.1957798564281053No Hit
GAGTTACAGTTGCATGCAAGGTTAAGCCGAAAAAGCGG90.1957798564281053No Hit
GGGTAGATCACCTGGTTTCGGGTCTACGGCAACATACT90.1957798564281053No Hit
GAGTGAAATAGTTCCTGAAACCATGTGCCTACACAGTC90.1957798564281053No Hit
AGTCTTAATAGGGCGTCATAGTATGTTGCCGTAGACCC90.1957798564281053No Hit
GTTCGGACCTCCGGTGCGTATTACCGCACTTTCATCCT80.1740265390472047No Hit
GGTTTCGGGTCTACGGCAACATACTATGACGCCCTATT80.1740265390472047No Hit
TCCTGAAACCATGTGCCTACACAGTCAGAGCCCGTTAA80.1740265390472047No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT80.1740265390472047No Hit
AGGTTAAGCCGAAAAAGCGGAGCCGTAGCGAAAGCGAG80.1740265390472047No Hit
CGCTACGGCTCCGCTTTTTCGGCTTAACCTTGCATGCA70.1522732216663041No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT70.1522732216663041No Hit
GCCTCTTGTAGAATGAACCGGCGAGTTACAGTTGCATG70.1522732216663041No Hit
ATCTACCCATGTCCAGGATGAAAGTGCGGTAATACGCA70.1522732216663041No Hit
GTATGTTGCCGTAGACCCGAAACCAGGTGATCTACCCA70.1522732216663041No Hit
TTACCGCACTTTCATCCTGGACATGGGTAGATCACCTG70.1522732216663041No Hit
GGTATCAACGCAGAGTACATGGGTTCGGACCTCCGGTG70.1522732216663041No Hit
GATCTACCCATGTCCAGGATGAAAGTGCGGTAATACGC60.13051990428540353No Hit
CCTCCGGTGCGTATTACCGCACTTTCATCCTGGACATG60.13051990428540353No Hit
ATGCAAGGTTAAGCCGAAAAAGCGGAGCCGTAGCGAAA60.13051990428540353No Hit
GTTAAGCCGAAAAAGCGGAGCCGTAGCGAAAGCGAGTC50.10876658690450293No Hit
GTATCAACGCAGAGTACATGGGCGGTGTGTACAAGACC50.10876658690450293No Hit
TACTATGACGCCCTATTAAGACTCGCTTTCGCTACGGC50.10876658690450293No Hit
CCCATTAACGGGCTCTGACTGTGTAGGCACATGGTTTC50.10876658690450293No Hit
ACATACTATGACGCCCTATTAAGACTCGCTTTCGCTAC50.10876658690450293No Hit
GTACATGGGCCGGAAGGGGAGTGAAATAGTTCCTGAAA50.10876658690450293No Hit
CTACGGCTCCGCTTTTTCGGCTTAACCTTGCATGCAAC50.10876658690450293No Hit
GTGCGTATTACCGCACTTTCATCCTGGACATGGGTAGA50.10876658690450293No Hit
GGGTGATGGCGTGCCTCTTGTAGAATGAACCGGCGAGT50.10876658690450293No Hit
ATCCTGGACATGGGTAGATCACCTGGTTTCGGGTCTAC50.10876658690450293No Hit
ACCTTGCATGCAACTGTAACTCGCCGGTTCATTCTACA50.10876658690450293No Hit
GTGATGGCGTGCCTCTTGTAGAATGAACCGGCGAGTTA50.10876658690450293No Hit
CTTTCATCCTGGACATGGGTAGATCACCTGGTTTCGGG50.10876658690450293No Hit
GTAGATCACCTGGTTTCGGGTCTACGGCAACATACTAT50.10876658690450293No Hit
GTCTTAATAGGGCGTCATAGTATGTTGCCGTAGACCCG50.10876658690450293No Hit
CCCATGTCCAGGATGAAAGTGCGGTAATACGCACCGGA50.10876658690450293No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT50.10876658690450293No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA200.003377067932.01
Produced by FastQC (version 0.11.5)