##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062876_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4597
Sequences flagged as poor quality	0
Sequence length	38
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.34587774635632	32.0	32.0	32.0	32.0	32.0
2	30.886447683271697	32.0	32.0	32.0	32.0	32.0
3	30.985860343702413	32.0	32.0	32.0	32.0	32.0
4	30.93430498150968	32.0	32.0	32.0	32.0	32.0
5	30.959321296497716	32.0	32.0	32.0	32.0	32.0
6	34.61039808570807	36.0	36.0	36.0	32.0	36.0
7	34.52708288013922	36.0	36.0	36.0	32.0	36.0
8	34.58146617359147	36.0	36.0	36.0	32.0	36.0
9	34.620622144877096	36.0	36.0	36.0	32.0	36.0
10	34.35001087665869	36.0	36.0	36.0	32.0	36.0
11	34.548183597998694	36.0	36.0	36.0	32.0	36.0
12	34.50859256036546	36.0	36.0	36.0	32.0	36.0
13	34.458994996737005	36.0	36.0	36.0	32.0	36.0
14	34.458124864041764	36.0	36.0	36.0	32.0	36.0
15	34.43115075048945	36.0	36.0	36.0	32.0	36.0
16	34.49467043724168	36.0	36.0	36.0	32.0	36.0
17	34.39242984555145	36.0	36.0	36.0	32.0	36.0
18	34.32303676310637	36.0	36.0	36.0	32.0	36.0
19	34.33804655209919	36.0	36.0	36.0	32.0	36.0
20	34.37829018925386	36.0	36.0	36.0	32.0	36.0
21	34.33434848814444	36.0	36.0	36.0	32.0	36.0
22	34.394387644115724	36.0	36.0	36.0	32.0	36.0
23	34.383510985425275	36.0	36.0	36.0	32.0	36.0
24	34.29149445290407	36.0	36.0	36.0	32.0	36.0
25	34.10789645420927	36.0	36.0	36.0	32.0	36.0
26	34.19730258864477	36.0	36.0	36.0	32.0	36.0
27	34.15401348705677	36.0	36.0	36.0	32.0	36.0
28	34.05112029584512	36.0	36.0	36.0	32.0	36.0
29	34.10550358929737	36.0	36.0	36.0	32.0	36.0
30	33.941918642592995	36.0	36.0	36.0	32.0	36.0
31	33.978681748966714	36.0	36.0	36.0	32.0	36.0
32	34.095932129649775	36.0	36.0	36.0	32.0	36.0
33	34.03437024146182	36.0	36.0	36.0	32.0	36.0
34	34.13117250380683	36.0	36.0	36.0	32.0	36.0
35	34.042201435718944	36.0	36.0	36.0	32.0	36.0
36	34.06787035022841	36.0	36.0	36.0	32.0	36.0
37	33.95975636284533	36.0	36.0	36.0	32.0	36.0
38	33.55840765716772	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	3.0
17	2.0
18	1.0
19	8.0
20	6.0
21	7.0
22	9.0
23	16.0
24	23.0
25	36.0
26	43.0
27	73.0
28	67.0
29	116.0
30	162.0
31	196.0
32	259.0
33	358.0
34	875.0
35	2336.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.389203308663475	17.17457553330431	12.38572050500653	22.050500653025686
2	25.070729053318825	16.60500544069641	35.952121871599566	22.372143634385203
3	26.865346965412222	22.94974983685012	25.777681096367193	24.40722210137046
4	22.231890363280403	15.270828801392211	33.67413530563411	28.823145529693278
5	22.819229932564717	32.86926256254078	29.279965194692192	15.031542310202306
6	36.697846421579285	32.89101587992169	17.032847509245162	13.37829018925386
7	34.283228192299326	29.279965194692192	18.6425929954318	17.79421361757668
8	34.60952795301284	29.32347182945399	17.032847509245162	19.034152708288012
9	34.39199477920383	14.248422884489884	17.902980204481185	33.4566021318251
10	25.12508157494018	23.01500978899282	27.191646726125736	24.668261909941265
11	41.657602784424625	21.057211224711768	20.143571894713943	17.141614096149663
12	31.41179029802045	21.057211224711768	24.77702849684577	22.753969980422013
13	37.21992603872091	19.795518816619534	21.709810746138785	21.274744398520777
14	31.303023711115944	17.533173809005874	23.972155753752446	27.191646726125736
15	32.06438981944746	24.84228844898847	22.297150315423103	20.796171416140965
16	32.825755927778985	23.906895801609746	22.079617141614097	21.18773112899717
17	31.607570154448556	23.537089406134434	22.471176854470308	22.384163584946705
18	30.43289101587992	23.64585599303894	23.537089406134434	22.384163584946705
19	33.82640852730041	22.1231237763759	23.950402436371547	20.100065259952142
20	33.478355449206006	21.10071785947357	23.01500978899282	22.405916902327604
21	35.04459430063084	21.90559060256689	21.53578420709158	21.514030889710682
22	30.998477267783336	23.558842723515337	24.124428975418752	21.318251033282575
23	31.477050250163153	20.665651511855557	24.646508592560366	23.210789645420927
24	32.08614313682837	21.078964542092667	25.299108113987383	21.53578420709158
25	32.84750924515989	20.622144877093756	23.73286926256254	22.797476615183815
26	32.934522514683486	23.863389166847945	22.253643680661302	20.948444637807263
27	32.73874265825538	22.2753969980422	22.884489884707417	22.101370458994996
28	32.30367631063737	22.993256471611918	23.68936262780074	21.013704589949967
29	32.716989340874484	22.1883837285186	22.927996519469218	22.166630411137696
30	32.76049597563628	21.601044159234284	23.602349358277134	22.036110506852292
31	31.368283663258644	23.68936262780074	24.015662388514247	20.926691320426364
32	31.91211659778116	23.40656950184903	23.036763106373723	21.644550793996086
33	31.17250380683054	22.862736567326518	23.167283010659126	22.797476615183815
34	32.716989340874484	21.81857733304329	24.907548401131173	20.556884924951056
35	32.08614313682837	20.991951272569068	24.49423537089406	22.427670219708506
36	32.43419621492278	22.819229932564717	22.797476615183815	21.949097237328694
37	31.281270393735046	22.144877093756797	23.145529693278224	23.428322819229933
38	31.21601044159234	23.68936262780074	23.972155753752446	21.12247117685447
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	2.0
8	4.0
9	4.0
10	4.0
11	4.0
12	5.0
13	6.0
14	12.0
15	18.0
16	18.0
17	18.0
18	18.0
19	18.0
20	25.0
21	32.0
22	37.5
23	43.0
24	43.0
25	49.0
26	55.0
27	55.0
28	66.0
29	77.0
30	106.5
31	136.0
32	136.0
33	147.0
34	158.0
35	158.0
36	171.0
37	184.0
38	198.5
39	213.0
40	213.0
41	215.0
42	217.0
43	262.5
44	308.0
45	308.0
46	379.5
47	451.0
48	451.0
49	439.5
50	428.0
51	426.0
52	424.0
53	424.0
54	393.0
55	362.0
56	362.0
57	317.0
58	272.0
59	228.0
60	184.0
61	184.0
62	157.5
63	131.0
64	120.0
65	109.0
66	109.0
67	110.0
68	111.0
69	111.0
70	93.0
71	75.0
72	59.0
73	43.0
74	43.0
75	39.0
76	35.0
77	35.0
78	28.0
79	21.0
80	21.0
81	21.0
82	21.0
83	13.0
84	5.0
85	5.0
86	4.0
87	3.0
88	2.0
89	1.0
90	1.0
91	0.5
92	0.0
93	1.5
94	3.0
95	3.0
96	5.5
97	8.0
98	8.0
99	222.5
100	437.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06525995214270176
2	0.04350663476180117
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	4597.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.61192081792474
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.16038882138517	65.28170546008266
2	4.76913730255164	6.830541657602785
3	1.275820170109356	2.740917989993474
4	0.8201701093560146	2.3493582771372634
5	0.5164034021871203	1.8490319773765498
6	0.09113001215066828	0.3915597128562106
7	0.212636695018226	1.0659125516641288
8	0.15188335358444716	0.8701326952360234
9	0.18226002430133656	1.1746791385686317
>10	0.7897934386391251	8.13574070045682
>50	0.0	0.0
>100	0.030376670716889428	9.310419839025451
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	428	9.310419839025451	No Hit
CTATTAAGACTCGCTTTCGCTACGGCTCCGCTTTTTCG	27	0.5873395692843159	No Hit
GTATCAACGCAGAGTACATGGGTTCGGACCTCCGGTGC	21	0.45681966499891236	No Hit
ATTAAGACTCGCTTTCGCTACGGCTCCGCTTTTTCGGC	19	0.41331303023711113	No Hit
AGTTACAGTTGCATGCAAGGTTAAGCCGAAAAAGCGGA	18	0.3915597128562106	No Hit
ATACTATGACGCCCTATTAAGACTCGCTTTCGCTACGG	18	0.3915597128562106	No Hit
GTTAATGGGTGATGGCGTGCCTCTTGTAGAATGAACCG	17	0.36980639547531	No Hit
TATCAACGCAGAGTACATGGGCCGGAAGGGGAGTGAAA	17	0.36980639547531	No Hit
CTCTTGTAGAATGAACCGGCGAGTTACAGTTGCATGCA	16	0.3480530780944094	No Hit
TGCCTACACAGTCAGAGCCCGTTAATGGGTGATGGCGT	15	0.3262997607135088	No Hit
GTATCAACGCAGAGTACATGGGCCGGAAGGGGAGTGAA	15	0.3262997607135088	No Hit
TTACAGTTGCATGCAAGGTTAAGCCGAAAAAGCGGAGC	14	0.3045464433326082	No Hit
GTACATGGGTTCGGACCTCCGGTGCGTATTACCGCACT	14	0.3045464433326082	No Hit
GGCTCTGACTGTGTAGGCACATGGTTTCAGGAACTATT	14	0.3045464433326082	No Hit
GTCAGAGCCCGTTAATGGGTGATGGCGTGCCTCTTGTA	13	0.28279312595170764	No Hit
GTGTAGGCACATGGTTTCAGGAACTATTTCACTCCCCT	13	0.28279312595170764	No Hit
TTCTACAAGAGGCACGCCATCACCCATTAACGGGCTCT	13	0.28279312595170764	No Hit
TATCAACGCAGAGTACATGGGTTCGGACCTCCGGTGCG	12	0.26103980857080705	No Hit
GCGTATTACCGCACTTTCATCCTGGACATGGGTAGATC	12	0.26103980857080705	No Hit
ACGCAGAGTACATGGGCCGGAAGGGGAGTGAAATAGTT	12	0.26103980857080705	No Hit
CTTGTAGAATGAACCGGCGAGTTACAGTTGCATGCAAG	11	0.23928649118990647	No Hit
TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT	11	0.23928649118990647	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	11	0.23928649118990647	No Hit
TCATAGTATGTTGCCGTAGACCCGAAACCAGGTGATCT	11	0.23928649118990647	No Hit
CTACAAGAGGCACGCCATCACCCATTAACGGGCTCTGA	10	0.21753317380900586	No Hit
GTTGCATGCAAGGTTAAGCCGAAAAAGCGGAGCCGTAG	10	0.21753317380900586	No Hit
ACTGTAACTCGCCGGTTCATTCTACAAGAGGCACGCCA	10	0.21753317380900586	No Hit
CCGTTAATGGGTGATGGCGTGCCTCTTGTAGAATGAAC	9	0.1957798564281053	No Hit
GGACATGGGTAGATCACCTGGTTTCGGGTCTACGGCAA	9	0.1957798564281053	No Hit
GAGTTACAGTTGCATGCAAGGTTAAGCCGAAAAAGCGG	9	0.1957798564281053	No Hit
GGGTAGATCACCTGGTTTCGGGTCTACGGCAACATACT	9	0.1957798564281053	No Hit
GAGTGAAATAGTTCCTGAAACCATGTGCCTACACAGTC	9	0.1957798564281053	No Hit
AGTCTTAATAGGGCGTCATAGTATGTTGCCGTAGACCC	9	0.1957798564281053	No Hit
GTTCGGACCTCCGGTGCGTATTACCGCACTTTCATCCT	8	0.1740265390472047	No Hit
GGTTTCGGGTCTACGGCAACATACTATGACGCCCTATT	8	0.1740265390472047	No Hit
TCCTGAAACCATGTGCCTACACAGTCAGAGCCCGTTAA	8	0.1740265390472047	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8	0.1740265390472047	No Hit
AGGTTAAGCCGAAAAAGCGGAGCCGTAGCGAAAGCGAG	8	0.1740265390472047	No Hit
CGCTACGGCTCCGCTTTTTCGGCTTAACCTTGCATGCA	7	0.1522732216663041	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	7	0.1522732216663041	No Hit
GCCTCTTGTAGAATGAACCGGCGAGTTACAGTTGCATG	7	0.1522732216663041	No Hit
ATCTACCCATGTCCAGGATGAAAGTGCGGTAATACGCA	7	0.1522732216663041	No Hit
GTATGTTGCCGTAGACCCGAAACCAGGTGATCTACCCA	7	0.1522732216663041	No Hit
TTACCGCACTTTCATCCTGGACATGGGTAGATCACCTG	7	0.1522732216663041	No Hit
GGTATCAACGCAGAGTACATGGGTTCGGACCTCCGGTG	7	0.1522732216663041	No Hit
GATCTACCCATGTCCAGGATGAAAGTGCGGTAATACGC	6	0.13051990428540353	No Hit
CCTCCGGTGCGTATTACCGCACTTTCATCCTGGACATG	6	0.13051990428540353	No Hit
ATGCAAGGTTAAGCCGAAAAAGCGGAGCCGTAGCGAAA	6	0.13051990428540353	No Hit
GTTAAGCCGAAAAAGCGGAGCCGTAGCGAAAGCGAGTC	5	0.10876658690450293	No Hit
GTATCAACGCAGAGTACATGGGCGGTGTGTACAAGACC	5	0.10876658690450293	No Hit
TACTATGACGCCCTATTAAGACTCGCTTTCGCTACGGC	5	0.10876658690450293	No Hit
CCCATTAACGGGCTCTGACTGTGTAGGCACATGGTTTC	5	0.10876658690450293	No Hit
ACATACTATGACGCCCTATTAAGACTCGCTTTCGCTAC	5	0.10876658690450293	No Hit
GTACATGGGCCGGAAGGGGAGTGAAATAGTTCCTGAAA	5	0.10876658690450293	No Hit
CTACGGCTCCGCTTTTTCGGCTTAACCTTGCATGCAAC	5	0.10876658690450293	No Hit
GTGCGTATTACCGCACTTTCATCCTGGACATGGGTAGA	5	0.10876658690450293	No Hit
GGGTGATGGCGTGCCTCTTGTAGAATGAACCGGCGAGT	5	0.10876658690450293	No Hit
ATCCTGGACATGGGTAGATCACCTGGTTTCGGGTCTAC	5	0.10876658690450293	No Hit
ACCTTGCATGCAACTGTAACTCGCCGGTTCATTCTACA	5	0.10876658690450293	No Hit
GTGATGGCGTGCCTCTTGTAGAATGAACCGGCGAGTTA	5	0.10876658690450293	No Hit
CTTTCATCCTGGACATGGGTAGATCACCTGGTTTCGGG	5	0.10876658690450293	No Hit
GTAGATCACCTGGTTTCGGGTCTACGGCAACATACTAT	5	0.10876658690450293	No Hit
GTCTTAATAGGGCGTCATAGTATGTTGCCGTAGACCCG	5	0.10876658690450293	No Hit
CCCATGTCCAGGATGAAAGTGCGGTAATACGCACCGGA	5	0.10876658690450293	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	5	0.10876658690450293	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.021753317380900587	0.0
13	0.0	0.0	0.0	0.021753317380900587	0.0
14	0.0	0.0	0.0	0.021753317380900587	0.0
15	0.0	0.0	0.0	0.021753317380900587	0.0
16	0.0	0.0	0.0	0.021753317380900587	0.0
17	0.0	0.0	0.0	0.04350663476180117	0.0
18	0.0	0.0	0.0	0.04350663476180117	0.0
19	0.0	0.0	0.0	0.04350663476180117	0.0
20	0.0	0.0	0.0	0.06525995214270176	0.0
21	0.0	0.0	0.0	0.06525995214270176	0.0
22	0.0	0.0	0.0	0.06525995214270176	0.0
23	0.0	0.0	0.0	0.06525995214270176	0.0
24	0.0	0.0	0.0	0.06525995214270176	0.0
25	0.0	0.0	0.0	0.06525995214270176	0.0
26	0.0	0.0	0.0	0.06525995214270176	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	20	0.0033770679	32.0	1
>>END_MODULE