FastQCFastQC Report
Thu 2 Feb 2017
SRR4062876_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062876_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4597
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAAT1823.959103763323907No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTC871.892538612138351No Hit
CTATTAAGACTCGCTTTCGCTACGGCTCCGCTTTTTCG450.9788992821405265No Hit
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT380.8266260604742223No Hit
TATCAACGCAGAGTACATGGGCCGGAAGGGGAGTGAAA220.47857298237981294No Hit
ATACTATGACGCCCTATTAAGACTCGCTTTCGCTACGG210.45681966499891236No Hit
ATTAAGACTCGCTTTCGCTACGGCTCCGCTTTTTCGGC200.4350663476180117No Hit
GTATCAACGCAGAGTACATGGGTTCGGACCTCCGGTGC170.36980639547531No Hit
TGCCTACACAGTCAGAGCCCGTTAATGGGTGATGGCGT170.36980639547531No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT160.3480530780944094No Hit
CTTGTAGAATGAACCGGCGAGTTACAGTTGCATGCAAG160.3480530780944094No Hit
TTATACACATCTCCGAGCCCACGAGACACTCGCTAATC150.3262997607135088No Hit
GTTAATGGGTGATGGCGTGCCTCTTGTAGAATGAACCG140.3045464433326082No Hit
GACCTCCGGTGCGTATTACCGCACTTTCATCCTGGACA140.3045464433326082No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA140.3045464433326082No Hit
CTCTTGTAGAATGAACCGGCGAGTTACAGTTGCATGCA140.3045464433326082No Hit
GGTATCAACGCAGAGTACATGGGCCGGAAGGGGAGTGA140.3045464433326082No Hit
GTCAGAGCCCGTTAATGGGTGATGGCGTGCCTCTTGTA130.28279312595170764No Hit
CACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTA130.28279312595170764No Hit
GTACATGGGTTCGGACCTCCGGTGCGTATTACCGCACT130.28279312595170764No Hit
TATCAACGCAGAGTACATGGGTTCGGACCTCCGGTGCG120.26103980857080705No Hit
GATCTACCCATGTCCAGGATGAAAGTGCGGTAATACGC110.23928649118990647No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT110.23928649118990647No Hit
ACGCAGAGTACATGGGCCGGAAGGGGAGTGAAATAGTT110.23928649118990647No Hit
GTGCCTACACAGTCAGAGCCCGTTAATGGGTGATGGCG100.21753317380900586No Hit
ACCTTGCATGCAACTGTAACTCGCCGGTTCATTCTACA100.21753317380900586No Hit
AGTTACAGTTGCATGCAAGGTTAAGCCGAAAAAGCGGA100.21753317380900586No Hit
TTACAGTTGCATGCAAGGTTAAGCCGAAAAAGCGGAGC100.21753317380900586No Hit
AGTCTTAATAGGGCGTCATAGTATGTTGCCGTAGACCC100.21753317380900586No Hit
GGACATGGGTAGATCACCTGGTTTCGGGTCTACGGCAA90.1957798564281053No Hit
GTGTAGGCACATGGTTTCAGGAACTATTTCACTCCCCT90.1957798564281053No Hit
ACATACTATGACGCCCTATTAAGACTCGCTTTCGCTAC90.1957798564281053No Hit
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATG90.1957798564281053No Hit
GCGTATTACCGCACTTTCATCCTGGACATGGGTAGATC90.1957798564281053No Hit
CTATGACGCCCTATTAAGACTCGCTTTCGCTACGGCTC90.1957798564281053No Hit
GGGTAGATCACCTGGTTTCGGGTCTACGGCAACATACT80.1740265390472047No Hit
GTATGTTGCCGTAGACCCGAAACCAGGTGATCTACCCA80.1740265390472047No Hit
CTTTCATCCTGGACATGGGTAGATCACCTGGTTTCGGG80.1740265390472047No Hit
GGTATCAACGCAGAGTACATGGGTTCGGACCTCCGGTG80.1740265390472047No Hit
ATGCAAGGTTAAGCCGAAAAAGCGGAGCCGTAGCGAAA80.1740265390472047No Hit
CCCTATTAAGACTCGCTTTCGCTACGGCTCCGCTTTTT80.1740265390472047No Hit
TCTTAATAGGGCGTCATAGTATGTTGCCGTAGACCCGA80.1740265390472047No Hit
GGCTCTGACTGTGTAGGCACATGGTTTCAGGAACTATT80.1740265390472047No Hit
CGCTACGGCTCCGCTTTTTCGGCTTAACCTTGCATGCA70.1522732216663041No Hit
GTACATGGGCCGGAAGGGGAGTGAAATAGTTCCTGAAA70.1522732216663041No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCGC70.1522732216663041No Hit
GCATATATTTAGGGACCTTAACTGGCGGTCTGGGCTGT70.1522732216663041No Hit
TTCTACAAGAGGCACGCCATCACCCATTAACGGGCTCT70.1522732216663041No Hit
GCTTAACCTTGCATGCAACTGTAACTCGCCGGTTCATT70.1522732216663041No Hit
ATATAAACCAATTGCATGCTTAATCTTGCTTGGCGTTG60.13051990428540353No Hit
ACGCAGAGTACATGGGTTCGGACCTCCGGTGCGTATTA60.13051990428540353No Hit
GTAATACGCACCGGAGGTCCGAACCCATGTACTCTGCG60.13051990428540353No Hit
GTATTACCGCACTTTCATCCTGGACATGGGTAGATCAC60.13051990428540353No Hit
GTATCAACGCAGAGTACATGGGGAGTTGTAATTTGTAG60.13051990428540353No Hit
GTATCAACGCAGAGTACATGGGCCGGAAGGGGAGTGAA60.13051990428540353No Hit
ACTGTAACTCGCCGGTTCATTCTACAAGAGGCACGCCA60.13051990428540353No Hit
AGGTTAAGCCGAAAAAGCGGAGCCGTAGCGAAAGCGAG60.13051990428540353No Hit
CCCATGTCCAGGATGAAAGTGCGGTAATACGCACCGGA60.13051990428540353No Hit
GTACTACATGTGGTACCGGGGAGAAGTGACTGGCGTCC50.10876658690450293No Hit
GAAGGGGAGTGAAATAGTTCCTGAAACCATGTGCCTAC50.10876658690450293No Hit
GTACATGGGGAGTTGTAATTTGTAGAATTTGTCTTTGA50.10876658690450293No Hit
GAGTTACAGTTGCATGCAAGGTTAAGCCGAAAAAGCGG50.10876658690450293No Hit
TACTATGACGCCCTATTAAGACTCGCTTTCGCTACGGC50.10876658690450293No Hit
CCCATTAACGGGCTCTGACTGTGTAGGCACATGGTTTC50.10876658690450293No Hit
ATATTTAGCTTTAGATGGAATTTACCACCCACTTTGAG50.10876658690450293No Hit
ATCCTGGACATGGGTAGATCACCTGGTTTCGGGTCTAC50.10876658690450293No Hit
GCGTCATAGTATGTTGCCGTAGACCCGAAACCAGGTGA50.10876658690450293No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCT50.10876658690450293No Hit
CTACAAGAGGCACGCCATCACCCATTAACGGGCTCTGA50.10876658690450293No Hit
TCCTGAAACCATGTGCCTACACAGTCAGAGCCCGTTAA50.10876658690450293No Hit
ACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTAT50.10876658690450293No Hit
TTACCGCACTTTCATCCTGGACATGGGTAGATCACCTG50.10876658690450293No Hit
GTCTTAATAGGGCGTCATAGTATGTTGCCGTAGACCCG50.10876658690450293No Hit
GCAACATACTATGACGCCCTATTAAGACTCGCTTTCGC50.10876658690450293No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATGTC50.10876658690450293No Hit
GTGAAATAGTTCCTGAAACCATGTGCCTACACAGTCAG50.10876658690450293No Hit
CATACTATGACGCCCTATTAAGACTCGCTTTCGCTACG50.10876658690450293No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACA252.4696466E-432.02
CTTATAC252.4696466E-432.01
TATACAC301.7970124E-531.9999983
TACACAT301.7970124E-531.9999985
AGCCCAC355.1549458E-527.4285717
ATCTCCG355.1549458E-527.4285710
CACATCT355.1549458E-527.428577
CCACGAG355.1549458E-527.4285720
CATCTCC355.1549458E-527.428579
ACACTCG355.1549458E-527.4285727
GAGCCCA355.1549458E-527.4285716
CACGAGA355.1549458E-527.4285721
CCCACGA355.1549458E-527.4285719
GACACTC355.1549458E-527.4285726
ACACATC355.1549458E-527.428576
ACGAGAC355.1549458E-527.4285722
CACTCGC355.1549458E-527.4285728
CGAGCCC355.1549458E-527.4285715
CCGAGCC355.1549458E-527.4285714
TCTCCGA355.1549458E-527.4285711
Produced by FastQC (version 0.11.5)