FastQCFastQC Report
Thu 2 Feb 2017
SRR4062875_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062875_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2291494
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT72850.3179148625307332No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT46740.20397173197922402No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA29690.12956612585500987No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG29420.12838785525949448No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG27770.12118731273134471No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG27300.11913624910211415No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC26600.11608147348411124No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC26540.1158196355739967No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA26360.11503412184365308No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG26130.11403040985488071No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT26060.11372493229308042No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT25440.11101927388856353No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC24650.1075717414053888No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG24350.10626255185481612No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA23880.10421148822558558No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGAC400.004484850419.99997322
CGTATAC507.205337E-419.1974583
TATGCGA550.001365019517.45490312
ACGTTTT1950.016.40987629
ACGATTA1304.809408E-915.99997932
CACGTTT2250.015.64374128
CGAGCCG8400.015.42888615
ATACCGT5950.015.0551886
AGGCCCG9250.015.04895810
GTATTAG6500.015.0261871
GTATTAC1951.8189894E-1214.7798571
ATGCGAA650.004162689714.76953313
ACCGTCG5800.014.6203518
TACGCTA7400.014.4867829
TACCGTC5750.014.4689147
CTAGCGG5800.014.34449629
ATCGTTT5600.014.28538329
GGCATCG5950.014.25145926
ATACGCT7550.014.1983458
CGTTTTT2400.013.99998130
Produced by FastQC (version 0.11.5)