##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062875_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2291494
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.194213905862288	32.0	32.0	32.0	32.0	32.0
2	30.781719262629533	32.0	32.0	32.0	32.0	32.0
3	30.834767623218738	32.0	32.0	32.0	32.0	32.0
4	30.904835884361905	32.0	32.0	32.0	32.0	32.0
5	30.819161647379396	32.0	32.0	32.0	32.0	32.0
6	34.462720391150924	36.0	36.0	36.0	32.0	36.0
7	34.37989364144091	36.0	36.0	36.0	32.0	36.0
8	34.36314561591695	36.0	36.0	36.0	32.0	36.0
9	34.49857080140729	36.0	36.0	36.0	32.0	36.0
10	34.21232697969098	36.0	36.0	36.0	32.0	36.0
11	34.508817391623104	36.0	36.0	36.0	32.0	36.0
12	34.307382868992896	36.0	36.0	36.0	32.0	36.0
13	34.40617343968607	36.0	36.0	36.0	32.0	36.0
14	34.297977651261576	36.0	36.0	36.0	32.0	36.0
15	34.238363268679734	36.0	36.0	36.0	32.0	36.0
16	34.252337994339065	36.0	36.0	36.0	32.0	36.0
17	34.177940243352154	36.0	36.0	36.0	32.0	36.0
18	34.20073017865201	36.0	36.0	36.0	32.0	36.0
19	34.202333063058425	36.0	36.0	36.0	32.0	36.0
20	34.17499325767381	36.0	36.0	36.0	32.0	36.0
21	34.16110362933527	36.0	36.0	36.0	32.0	36.0
22	34.1306427160621	36.0	36.0	36.0	32.0	36.0
23	34.078869942491664	36.0	36.0	36.0	32.0	36.0
24	34.062358007483326	36.0	36.0	36.0	32.0	36.0
25	34.04329489843744	36.0	36.0	36.0	32.0	36.0
26	33.99297183409601	36.0	36.0	36.0	32.0	36.0
27	33.99775866748942	36.0	36.0	36.0	32.0	36.0
28	33.982269645916595	36.0	36.0	36.0	32.0	36.0
29	33.9429935230029	36.0	36.0	36.0	32.0	36.0
30	33.91630394842841	36.0	36.0	36.0	32.0	36.0
31	33.93323613328248	36.0	36.0	36.0	32.0	36.0
32	33.88489256354151	36.0	36.0	36.0	32.0	36.0
33	33.84023479878193	36.0	36.0	36.0	32.0	36.0
34	33.847173939796484	36.0	36.0	36.0	32.0	36.0
35	33.79693815475842	36.0	36.0	36.0	32.0	36.0
36	33.75120816375692	36.0	36.0	36.0	32.0	36.0
37	33.7616118567188	36.0	36.0	36.0	32.0	36.0
38	33.14989914876495	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	7.0
10	29.0
11	23.0
12	14.0
13	15.0
14	232.0
15	532.0
16	797.0
17	913.0
18	1190.0
19	1594.0
20	2130.0
21	3061.0
22	4823.0
23	7319.0
24	11080.0
25	16747.0
26	24213.0
27	34352.0
28	47657.0
29	64001.0
30	85665.0
31	113736.0
32	153789.0
33	227994.0
34	495999.0
35	993576.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.281751801855336	17.499842709039687	11.486521912378448	25.73188357672653
2	16.52682003294841	20.20568087197582	37.5836754725582	25.683823622517572
3	18.570341189707833	24.702756350552413	28.602952665350916	28.123949794388835
4	12.418112968426987	15.746050809563855	36.508381924039206	35.32745429796994
5	14.481158094652907	36.58836528927928	33.596859333324026	15.33361728274379
6	33.2676118171301	36.07351907298623	17.251810723778927	13.407058386104742
7	29.694950106786226	30.828315500717	21.474199801526865	18.00253459096991
8	27.944169004668147	32.429684305431365	20.01089249033488	19.61525419956561
9	27.72301726429014	14.373729267337932	18.861196608499352	39.04205685987258
10	15.895355286763573	26.281100438274365	31.59843368083694	26.225110594125123
11	36.89023631854366	21.504721264915833	22.702744692095497	18.90229772444501
12	25.118572348771156	23.916352597594166	28.662214130227966	22.302860923406715
13	29.28128109778453	19.58270046613942	25.409792419560397	25.726226016515653
14	23.82785410308346	20.136700346398907	25.344380390251946	30.691065160265683
15	25.11870559179943	26.97696426217458	22.877245454093085	25.02708469193291
16	25.70144546626205	25.868604371532705	23.824857620329315	24.605092541875926
17	23.9019938115414	25.70541138758441	25.495462975532064	24.897131825342125
18	24.883715449588497	24.55334425551266	26.608584763123417	23.954355531775423
19	25.421821830384438	25.09755332209377	25.820370957936305	23.66025388958548
20	25.696041938613455	24.151056866500912	25.384522392704035	24.768378802181598
21	27.03150217296998	24.2610636424022	24.58541618114164	24.12201800348618
22	25.75996487618725	24.328346549133403	25.099429456257365	24.812259118421977
23	24.040824609986185	24.116590616973593	25.881772732529846	25.960812040510373
24	24.572881604491684	25.296224458442158	25.497781044428	24.633112892638167
25	24.755228029009494	24.498584093662814	25.591372224491867	25.154815652835826
26	24.39491524535984	25.394005632268396	26.0494595571354	24.16161956523637
27	25.210645899289748	25.002509507761143	24.89401148525326	24.892833107695854
28	24.14681450356427	24.739564467122932	26.115563112465377	24.99805791684742
29	24.19661616430536	25.110092936332457	26.025708282742226	24.667582616619953
30	24.32700058173509	25.039615155835616	26.24794832551504	24.385435936914256
31	24.921313106626634	25.030942322463517	24.915246840304448	25.1324977306054
32	24.319404505400556	25.120682709978748	24.89993842028613	25.659974364334566
33	24.120840969070517	24.659266820843456	25.57476970587555	25.645122504210477
34	24.935230899225562	24.827471526036827	25.84466065181109	24.392636922926517
35	25.72625608104982	24.653424897888993	25.668206224515895	23.95211279654529
36	24.440447441275406	25.36321105326077	25.19844742468993	24.997894080773897
37	25.3960903778254	25.2343023737927	24.99587564974883	24.37373159863307
38	24.285410659475566	25.09881724799038	25.715222154964472	24.900549937569583
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	906.0
1	710.5
2	515.0
3	515.0
4	1179.5
5	1844.0
6	1844.0
7	2174.0
8	2504.0
9	2392.0
10	2280.0
11	2280.0
12	3056.5
13	3833.0
14	4873.0
15	5913.0
16	5913.0
17	8816.5
18	11720.0
19	11720.0
20	14158.5
21	16597.0
22	17721.5
23	18846.0
24	18846.0
25	22273.0
26	25700.0
27	25700.0
28	32265.0
29	38830.0
30	47011.5
31	55193.0
32	55193.0
33	69906.0
34	84619.0
35	84619.0
36	92864.5
37	101110.0
38	114442.5
39	127775.0
40	127775.0
41	136226.0
42	144677.0
43	161964.5
44	179252.0
45	179252.0
46	185079.0
47	190906.0
48	190906.0
49	201901.0
50	212896.0
51	212046.0
52	211196.0
53	211196.0
54	200205.0
55	189214.0
56	189214.0
57	183707.5
58	178201.0
59	159016.0
60	139831.0
61	139831.0
62	133137.0
63	126443.0
64	103781.0
65	81119.0
66	81119.0
67	67504.5
68	53890.0
69	53890.0
70	43310.0
71	32730.0
72	25517.5
73	18305.0
74	18305.0
75	13627.0
76	8949.0
77	8949.0
78	9040.5
79	9132.0
80	7143.5
81	5155.0
82	5155.0
83	5380.5
84	5606.0
85	5606.0
86	3736.5
87	1867.0
88	1607.5
89	1348.0
90	1348.0
91	983.5
92	619.0
93	554.5
94	490.0
95	490.0
96	429.5
97	369.0
98	369.0
99	741.5
100	1114.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11965992492234324
2	0.054418645652137866
3	0.012044543865268684
4	0.003272973876431708
5	2.6183791011453664E-4
6	3.054775618002927E-4
7	0.0
8	3.054775618002927E-4
9	4.3639651685756106E-5
10	3.054775618002927E-4
11	0.002312901539345074
12	0.0028802170112599028
13	0.01457564366304254
14	0.008946128595580001
15	0.023216294696822248
16	0.011389949089982344
17	0.019856041517019026
18	0.0057604340225198055
19	0.007986056258493367
20	0.006327749494434635
21	0.006982344269720977
22	0.007462380438264294
23	0.009993480236038148
24	0.014226526449556489
25	0.01667034694395883
26	0.017892257191160003
27	0.00907704755063727
28	0.006109551236005855
29	0.010822633618067514
30	0.003185694573060196
31	0.0058477133258913174
32	0.006764146011292197
33	0.00807333556186488
34	0.012131823168640197
35	0.015710274606872198
36	0.014532004011356781
37	0.009775281977609367
38	0.0062404701910631225
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2291494.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.62614290821245
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.54937502238968	38.48476367303672
2	13.235360632366108	13.136397963670591
3	4.174988072780598	6.215656642196773
4	1.6911332968401946	3.3569769066331183
5	0.9060979520459969	2.248307322853664
6	0.5512902386537627	1.6415044900400504
7	0.34452330194089914	1.1968153832129802
8	0.25665834848764685	1.0189571104507005
9	0.1752345633264409	0.7826593933886544
>10	0.9282251544717737	8.213751444315374
>50	0.07861936932600469	2.7532448953224073
>100	0.08552747810314329	9.262513489339861
>500	0.015281601859001596	5.122308518883354
>1k	0.007596634450139507	6.246795755846621
>5k	8.83329587225524E-5	0.3193470108091945
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	7285	0.3179148625307332	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	4674	0.20397173197922402	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2969	0.12956612585500987	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2942	0.12838785525949448	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2777	0.12118731273134471	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2730	0.11913624910211415	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2660	0.11608147348411124	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2654	0.1158196355739967	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2636	0.11503412184365308	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2613	0.11403040985488071	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2606	0.11372493229308042	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2544	0.11101927388856353	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2465	0.1075717414053888	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2435	0.10626255185481612	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	2388	0.10421148822558558	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.4911721348604885E-4
2	0.0	0.0	0.0	0.0	3.4911721348604885E-4
3	0.0	0.0	0.0	0.0	3.4911721348604885E-4
4	0.0	0.0	0.0	0.0	3.4911721348604885E-4
5	0.0	0.0	0.0	0.0	3.4911721348604885E-4
6	0.0	0.0	0.0	0.0	5.673154719148294E-4
7	0.0	0.0	0.0	0.0	5.673154719148294E-4
8	0.0	0.0	0.0	4.3639651685756106E-5	6.109551236005854E-4
9	0.0	0.0	0.0	4.3639651685756106E-5	6.109551236005854E-4
10	0.0	0.0	0.0	4.3639651685756106E-5	6.109551236005854E-4
11	0.0	0.0	0.0	4.3639651685756106E-5	6.109551236005854E-4
12	0.0	0.0	0.0	4.3639651685756106E-5	0.0010473516404581466
13	0.0	0.0	0.0	4.3639651685756106E-5	0.001178270595515415
14	0.0	0.0	0.0	4.3639651685756106E-5	0.001178270595515415
15	0.0	0.0	0.0	4.3639651685756106E-5	0.0013964688539441954
16	0.0	0.0	0.0	8.727930337151221E-5	0.0013964688539441954
17	0.0	0.0	0.0	8.727930337151221E-5	0.0013964688539441954
18	0.0	0.0	0.0	1.7455860674302443E-4	0.0015273878090014638
19	0.0	0.0	0.0	2.1819825842878053E-4	0.0015273878090014638
20	0.0	0.0	0.0	3.9275686517180493E-4	0.001701946415744488
21	0.0	0.0	0.0	6.545947752863416E-4	0.001701946415744488
22	0.0	0.0	0.0	8.291533820293659E-4	0.0017455860674302443
23	0.0	0.0	0.0	0.001701946415744488	0.0017455860674302443
24	0.0	0.0	0.0	0.002923856662945659	0.0017892257191160002
25	0.0	0.0	0.0	0.0035784514382320005	0.0017892257191160002
26	0.0	0.0	0.0	0.0044948841236328784	0.0017892257191160002
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCGAC	40	0.0044848504	19.999973	22
CGTATAC	50	7.205337E-4	19.197458	3
TATGCGA	55	0.0013650195	17.454903	12
ACGTTTT	195	0.0	16.409876	29
ACGATTA	130	4.809408E-9	15.999979	32
CACGTTT	225	0.0	15.643741	28
CGAGCCG	840	0.0	15.428886	15
ATACCGT	595	0.0	15.055188	6
AGGCCCG	925	0.0	15.048958	10
GTATTAG	650	0.0	15.026187	1
GTATTAC	195	1.8189894E-12	14.779857	1
ATGCGAA	65	0.0041626897	14.769533	13
ACCGTCG	580	0.0	14.620351	8
TACGCTA	740	0.0	14.486782	9
TACCGTC	575	0.0	14.468914	7
CTAGCGG	580	0.0	14.344496	29
ATCGTTT	560	0.0	14.285383	29
GGCATCG	595	0.0	14.251459	26
ATACGCT	755	0.0	14.198345	8
CGTTTTT	240	0.0	13.999981	30
>>END_MODULE