FastQCFastQC Report
Thu 2 Feb 2017
SRR4062875_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062875_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2291494
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT77210.33694175066572285No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT54500.23783610168737077No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG30960.1351083616191009No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT30870.1347156047539291No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA29460.12856241386623748No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG28540.12454756591114792No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA28250.12328201601226099No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC28090.12258378158528889No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA27860.1215800695965165No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC27480.11992176283245777No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC26670.11638695104591154No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT26080.11381221159645191No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG25500.11128111179867807No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG24870.10853181374247543No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG24640.10752810175370305No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG24600.10735354314696001No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA23430.10224770389972657No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG5600.017.1444231
ATCGTTT5200.015.69134429
GCGTAAC2800.015.42930811
GTCTAAA756.2433793E-414.9346981
CGCGTAA2800.014.858500510
CATCGTT5550.014.701828
TCTAGCG6650.014.67579128
TCGCGTA2850.014.5981449
CTAGCGG6800.014.5873429
TATGCCG901.5331915E-414.2244535
CCTATAC1351.319313E-714.2232113
TAGCGGC7000.014.17055930
ATTAGAG7250.014.125123
CGTTTAT6100.013.90078531
CGAACGA3200.013.49946616
TAACGAA3200.013.49946613
TAAGACT2750.013.3833334
TATACTG3250.013.2943935
ACCGTCG4950.013.2546048
TACACCG1453.1495256E-713.2434565
Produced by FastQC (version 0.11.5)