##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062875_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2291494
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.193724705366893	32.0	32.0	32.0	32.0	32.0
2	31.291975889965236	32.0	32.0	32.0	32.0	32.0
3	31.374145426520865	32.0	32.0	32.0	32.0	32.0
4	31.475625072550923	32.0	32.0	32.0	32.0	32.0
5	31.39896809679624	32.0	32.0	32.0	32.0	32.0
6	34.92246324886733	36.0	36.0	36.0	36.0	36.0
7	34.949973248893514	36.0	36.0	36.0	36.0	36.0
8	34.893470373476866	36.0	36.0	36.0	32.0	36.0
9	34.99486666777221	36.0	36.0	36.0	36.0	36.0
10	34.84840981473222	36.0	36.0	36.0	32.0	36.0
11	35.022645488052774	36.0	36.0	36.0	36.0	36.0
12	34.915961813559186	36.0	36.0	36.0	32.0	36.0
13	34.96374941413767	36.0	36.0	36.0	36.0	36.0
14	34.91875867883573	36.0	36.0	36.0	32.0	36.0
15	34.88773917802098	36.0	36.0	36.0	32.0	36.0
16	34.89565061047509	36.0	36.0	36.0	32.0	36.0
17	34.86146854410267	36.0	36.0	36.0	32.0	36.0
18	34.85938387794164	36.0	36.0	36.0	32.0	36.0
19	34.851722064295174	36.0	36.0	36.0	32.0	36.0
20	34.832408463648605	36.0	36.0	36.0	32.0	36.0
21	34.82141258061335	36.0	36.0	36.0	32.0	36.0
22	34.807585356976716	36.0	36.0	36.0	32.0	36.0
23	34.75202073407131	36.0	36.0	36.0	32.0	36.0
24	34.72226983793106	36.0	36.0	36.0	32.0	36.0
25	34.7071164052797	36.0	36.0	36.0	32.0	36.0
26	34.64177737318972	36.0	36.0	36.0	32.0	36.0
27	34.62307647325282	36.0	36.0	36.0	32.0	36.0
28	34.59414294778865	36.0	36.0	36.0	32.0	36.0
29	34.551823831962906	36.0	36.0	36.0	32.0	36.0
30	34.53380851095399	36.0	36.0	36.0	32.0	36.0
31	34.5220869005112	36.0	36.0	36.0	32.0	36.0
32	34.49895221196302	36.0	36.0	36.0	32.0	36.0
33	34.450224613287226	36.0	36.0	36.0	32.0	36.0
34	34.45001208818352	36.0	36.0	36.0	32.0	36.0
35	34.40463426917112	36.0	36.0	36.0	32.0	36.0
36	34.3763260999156	36.0	36.0	36.0	32.0	36.0
37	34.36877513098442	36.0	36.0	36.0	32.0	36.0
38	33.933970152223836	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	9.0
20	20.0
21	43.0
22	169.0
23	534.0
24	1568.0
25	3620.0
26	7988.0
27	15436.0
28	26691.0
29	42511.0
30	64468.0
31	93047.0
32	133833.0
33	213652.0
34	516573.0
35	1171330.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.75362160713686	18.782822280810553	12.093568895093956	26.36998721695863
2	15.640719827640757	21.21049464257582	36.85466200420425	26.294123525579177
3	17.500460398325284	25.531727335965094	29.002432474184964	27.965379791524658
4	11.947574630610879	16.598661305371273	36.65647531776323	34.797288746254615
5	13.950037791971345	37.15897764425972	33.372667240383116	15.51831732338581
6	33.274521501085104	36.482042898744005	16.9955867123953	13.24784888777559
7	29.453427956230943	30.971703028869523	21.473395819823153	18.101473195076373
8	27.763407852801542	32.66487361424294	19.851691318591783	19.72002721436374
9	27.56661642086373	14.153430956244256	18.74140809862259	39.53854452426942
10	15.679624975507064	26.559419972358437	31.64893210798101	26.112022944153484
11	36.96508786622565	21.396048932936075	22.70674322602832	18.932119974809954
12	24.936995060412738	24.113250478837543	28.566646897844063	22.38310756290566
13	29.25501343884833	19.575638743508048	25.4110969669966	25.758250850647023
14	23.686075547219414	20.164879331999124	25.372093490098603	30.776951630682863
15	25.091359610804133	27.13386986830426	22.74254263812168	25.03222788276993
16	25.61936693675259	25.880201248705536	23.736882460474458	24.76354935406741
17	23.84344013119825	25.930200995507736	25.380952339390806	24.84540653390321
18	24.904538261937407	24.484768452372123	26.586584996513192	24.024108289177278
19	25.41486034875064	25.047894517725116	25.856188146248694	23.68105698727555
20	25.688742802730445	24.090178721829513	25.430526983705825	24.790551491734213
21	27.004807348225523	24.325199477021958	24.601831470500443	24.068161704252077
22	25.67770197085395	24.3733127819667	25.130940774883108	24.81804447229624
23	24.00093389017626	24.03248541342096	25.89315249030107	26.073428206101706
24	24.518981939293756	25.305106624760963	25.500699543616523	24.67521189232876
25	24.722538852415806	24.492426768236587	25.669127363307396	25.115907016040207
26	24.494238256787927	25.269933065502244	26.045889712126673	24.189938965583153
27	25.2983098791923	24.881332825367565	24.930427454938766	24.889929840501367
28	24.18841157777415	24.637201755710468	26.19282441935262	24.981562247162767
29	24.3154029641797	24.9394936229377	26.016869343755644	24.72823406912695
30	24.360700922629515	24.91296071471276	26.362931781623693	24.363406581034035
31	24.893671988667656	24.828779826610937	25.028300314118212	25.2492478706032
32	24.381691595090366	25.00621865036522	24.987540879443717	25.6245488751007
33	24.04186962741338	24.528189905799447	25.773491006304184	25.65644946048299
34	24.889046185588963	24.601242682721402	26.00067903298023	24.5090320987094
35	25.807093538102215	24.495372887731758	25.649248917954836	24.048284656211187
36	24.534081258777025	25.19552745937803	25.27329331868205	24.997097963162897
37	25.49812480416706	25.135130181444943	25.02424182651144	24.342503187876556
38	24.412555482386374	24.89721766551327	25.70935193779776	24.980874914302596
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	673.0
1	500.5
2	328.0
3	328.0
4	1070.0
5	1812.0
6	1812.0
7	2160.5
8	2509.0
9	2476.0
10	2443.0
11	2443.0
12	3069.5
13	3696.0
14	4780.0
15	5864.0
16	5864.0
17	9068.5
18	12273.0
19	12273.0
20	14482.5
21	16692.0
22	17950.5
23	19209.0
24	19209.0
25	22028.0
26	24847.0
27	24847.0
28	32072.5
29	39298.0
30	47164.5
31	55031.0
32	55031.0
33	70351.5
34	85672.0
35	85672.0
36	93724.5
37	101777.0
38	115172.5
39	128568.0
40	128568.0
41	136489.0
42	144410.0
43	162251.5
44	180093.0
45	180093.0
46	185843.0
47	191593.0
48	191593.0
49	202592.5
50	213592.0
51	212024.0
52	210456.0
53	210456.0
54	199981.5
55	189507.0
56	189507.0
57	184276.5
58	179046.0
59	159802.0
60	140558.0
61	140558.0
62	133293.0
63	126028.0
64	103947.5
65	81867.0
66	81867.0
67	67829.5
68	53792.0
69	53792.0
70	43079.0
71	32366.0
72	25306.5
73	18247.0
74	18247.0
75	13415.0
76	8583.0
77	8583.0
78	8746.5
79	8910.0
80	6832.5
81	4755.0
82	4755.0
83	4643.5
84	4532.0
85	4532.0
86	2884.0
87	1236.0
88	1081.0
89	926.0
90	926.0
91	550.5
92	175.0
93	120.5
94	66.0
95	66.0
96	46.5
97	27.0
98	27.0
99	32.0
100	37.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007724218348378831
2	4.3639651685756106E-5
3	0.0
4	3.4911721348604885E-4
5	8.727930337151221E-5
6	2.1819825842878053E-4
7	5.236758202290733E-4
8	0.002487460146088098
9	0.0040148479550895615
10	7.418740786578538E-4
11	0.00493128064049044
12	3.054775618002927E-4
13	1.3091895505726832E-4
14	0.0
15	0.0
16	4.3639651685756106E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	8.727930337151221E-5
22	0.0
23	1.7455860674302443E-4
24	0.0
25	8.727930337151221E-5
26	0.0
27	4.3639651685756106E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	4.3639651685756106E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2291494.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.40300375316264
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.83914482759627	35.950032668991916
2	14.233616920708242	13.494323926268242
3	4.425955758667559	6.294107923183503
4	1.8718778195007562	3.54930525213025
5	0.9876452165565597	2.3408674953611865
6	0.5695109710892975	1.6197918420007962
7	0.3787058551362987	1.2566256550659445
8	0.2743452374140973	1.0403830655042199
9	0.1934132782720903	0.8251533320259049
>10	1.025728712035686	8.746010613414857
>50	0.08274032845965304	2.773319203902104
>100	0.09200867046172194	9.562617782531442
>500	0.01625520847406917	5.199870100872397
>1k	0.008866477349492268	6.770951506697147
>5k	1.8471827811442223E-4	0.5766396320500994
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	7721	0.33694175066572285	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	5450	0.23783610168737077	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3096	0.1351083616191009	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	3087	0.1347156047539291	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2946	0.12856241386623748	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2854	0.12454756591114792	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	2825	0.12328201601226099	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2809	0.12258378158528889	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2786	0.1215800695965165	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2748	0.11992176283245777	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2667	0.11638695104591154	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2608	0.11381221159645191	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2550	0.11128111179867807	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2487	0.10853181374247543	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2464	0.10752810175370305	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2460	0.10735354314696001	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2343	0.10224770389972657	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.1819825842878053E-4
2	0.0	0.0	0.0	0.0	2.1819825842878053E-4
3	0.0	0.0	0.0	0.0	2.1819825842878053E-4
4	0.0	0.0	0.0	0.0	2.1819825842878053E-4
5	0.0	0.0	0.0	0.0	2.1819825842878053E-4
6	0.0	0.0	0.0	0.0	2.6183791011453664E-4
7	0.0	0.0	0.0	0.0	2.6183791011453664E-4
8	0.0	0.0	0.0	0.0	2.6183791011453664E-4
9	0.0	0.0	0.0	0.0	2.6183791011453664E-4
10	0.0	0.0	0.0	0.0	3.4911721348604885E-4
11	0.0	0.0	0.0	0.0	3.4911721348604885E-4
12	4.3639651685756106E-5	0.0	0.0	0.0	7.418740786578538E-4
13	4.3639651685756106E-5	0.0	0.0	0.0	8.291533820293659E-4
14	4.3639651685756106E-5	0.0	0.0	0.0	9.164326854008782E-4
15	4.3639651685756106E-5	0.0	0.0	0.0	9.600723370866343E-4
16	4.3639651685756106E-5	0.0	0.0	4.3639651685756106E-5	0.001178270595515415
17	4.3639651685756106E-5	0.0	0.0	4.3639651685756106E-5	0.001178270595515415
18	4.3639651685756106E-5	0.0	0.0	1.3091895505726832E-4	0.0012219102472011709
19	4.3639651685756106E-5	0.0	0.0	2.1819825842878053E-4	0.0012219102472011709
20	4.3639651685756106E-5	0.0	0.0	3.9275686517180493E-4	0.0013528292022584392
21	4.3639651685756106E-5	0.0	0.0	6.545947752863416E-4	0.0014401085056299514
22	4.3639651685756106E-5	0.0	0.0	8.291533820293659E-4	0.0014837481573157076
23	4.3639651685756106E-5	0.0	0.0	0.001701946415744488	0.0015273878090014638
24	4.3639651685756106E-5	0.0	0.0	0.002923856662945659	0.001614667112372976
25	4.3639651685756106E-5	0.0	0.0	0.0035348117865462445	0.001614667112372976
26	4.3639651685756106E-5	0.0	0.0	0.0043203255168898546	0.0017455860674302443
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAG	560	0.0	17.144423	1
ATCGTTT	520	0.0	15.691344	29
GCGTAAC	280	0.0	15.429308	11
GTCTAAA	75	6.2433793E-4	14.934698	1
CGCGTAA	280	0.0	14.8585005	10
CATCGTT	555	0.0	14.7018	28
TCTAGCG	665	0.0	14.675791	28
TCGCGTA	285	0.0	14.598144	9
CTAGCGG	680	0.0	14.58734	29
TATGCCG	90	1.5331915E-4	14.224453	5
CCTATAC	135	1.319313E-7	14.223211	3
TAGCGGC	700	0.0	14.170559	30
ATTAGAG	725	0.0	14.12512	3
CGTTTAT	610	0.0	13.900785	31
CGAACGA	320	0.0	13.499466	16
TAACGAA	320	0.0	13.499466	13
TAAGACT	275	0.0	13.383333	4
TATACTG	325	0.0	13.294393	5
ACCGTCG	495	0.0	13.254604	8
TACACCG	145	3.1495256E-7	13.243456	5
>>END_MODULE