FastQCFastQC Report
Thu 2 Feb 2017
SRR4062874_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062874_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1784932
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT75270.421696736906504No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT50040.28034681433242276No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT26660.14936143225624282No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT24910.13955713719066049No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC24050.13473902647271718No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG24020.13457095284302148No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG23780.1332263638054559No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA23540.13188177476789031No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG23540.13188177476789031No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22650.1268955904202513No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG21950.12297387239401839No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC21930.12286182330755457No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC21260.11910817891101734No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA20750.11625092720619049No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG19670.11020027653714538No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT19530.1094159329318988No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA18670.1045978222139555No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT17880.10017188329863545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG9200.017.9111614
CGGTAAC450.0088532117.77740329
TCTAGCG5800.017.37894628
AGGACGT9400.017.3593925
GGACGTG9050.016.9705926
TTAGGAC5450.016.7340623
TAGCGGC6100.016.52424230
CTAGCGG6150.016.389929
GACGTGA4900.016.3252747
TGTAGGA10250.016.0808662
GTATTAG5200.016.0122261
ATCGTTT4950.015.83805129
GTCCTAC7450.015.6898311
GTAGGAC9950.015.11568453
CATCGTT5100.015.05850728
CTGTAGG11000.014.9932651
TCTAGTA756.2384567E-414.9359482
GTACGAA756.2464183E-414.93343719
AGCGGCG7000.014.85683131
AAGACGG6300.014.7281995
Produced by FastQC (version 0.11.5)