##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062874_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1784932
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.143463168344788	32.0	32.0	32.0	32.0	32.0
2	30.742003616944512	32.0	32.0	32.0	32.0	32.0
3	30.793851530478474	32.0	32.0	32.0	32.0	32.0
4	30.85894476652332	32.0	32.0	32.0	32.0	32.0
5	30.777280030835907	32.0	32.0	32.0	32.0	32.0
6	34.41455752936246	36.0	36.0	36.0	32.0	36.0
7	34.32356134575435	36.0	36.0	36.0	32.0	36.0
8	34.29261787003651	36.0	36.0	36.0	32.0	36.0
9	34.43303162249318	36.0	36.0	36.0	32.0	36.0
10	34.142109055134874	36.0	36.0	36.0	32.0	36.0
11	34.45365313636598	36.0	36.0	36.0	32.0	36.0
12	34.25463322972528	36.0	36.0	36.0	32.0	36.0
13	34.3517747454805	36.0	36.0	36.0	32.0	36.0
14	34.235554631773084	36.0	36.0	36.0	32.0	36.0
15	34.18039958945215	36.0	36.0	36.0	32.0	36.0
16	34.19908489511085	36.0	36.0	36.0	32.0	36.0
17	34.11966674360704	36.0	36.0	36.0	32.0	36.0
18	34.1488527294037	36.0	36.0	36.0	32.0	36.0
19	34.13002624189605	36.0	36.0	36.0	32.0	36.0
20	34.10616875040618	36.0	36.0	36.0	32.0	36.0
21	34.08999838649316	36.0	36.0	36.0	32.0	36.0
22	34.06369038148232	36.0	36.0	36.0	32.0	36.0
23	34.01516360287115	36.0	36.0	36.0	32.0	36.0
24	33.99675169698342	36.0	36.0	36.0	32.0	36.0
25	33.97360571719259	36.0	36.0	36.0	32.0	36.0
26	33.92441784897128	36.0	36.0	36.0	32.0	36.0
27	33.93226352600547	36.0	36.0	36.0	32.0	36.0
28	33.915492578989	36.0	36.0	36.0	32.0	36.0
29	33.875200847987486	36.0	36.0	36.0	32.0	36.0
30	33.832373446159295	36.0	36.0	36.0	32.0	36.0
31	33.85963722987766	36.0	36.0	36.0	32.0	36.0
32	33.80611866446453	36.0	36.0	36.0	32.0	36.0
33	33.766364208832606	36.0	36.0	36.0	32.0	36.0
34	33.76484706420189	36.0	36.0	36.0	32.0	36.0
35	33.72977626038415	36.0	36.0	36.0	27.0	36.0
36	33.66732122008009	36.0	36.0	36.0	27.0	36.0
37	33.66367178133397	36.0	36.0	36.0	27.0	36.0
38	33.04001833123055	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	7.0
10	14.0
11	13.0
12	13.0
13	11.0
14	233.0
15	493.0
16	631.0
17	836.0
18	1065.0
19	1400.0
20	1966.0
21	2737.0
22	4110.0
23	6113.0
24	8998.0
25	13397.0
26	19405.0
27	27712.0
28	38516.0
29	51591.0
30	68552.0
31	92018.0
32	123415.0
33	182374.0
34	392063.0
35	747243.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.30122022771152	17.69588205400324	11.514419598133703	25.488478120151537
2	16.315331874740387	20.098368136078538	38.31973680301316	25.266563186167918
3	18.76336386008795	24.80808712452316	28.604600081583985	27.823948933804903
4	12.288575973143006	15.506417586579571	36.56189473578055	35.643111704496874
5	14.277870810113377	36.88259941745386	33.515563416461106	15.323966355971658
6	33.28851359830761	36.136079438498356	17.24142374370283	13.333983219491206
7	29.643370167603024	30.914006808102496	21.345631094069688	18.096991930224792
8	27.90332601837498	32.92734100610277	19.842268059141915	19.327064916380333
9	27.82928620690235	14.16291628406508	19.052746635860586	38.955050873171984
10	16.047042820238584	26.367857060820977	31.514229993719635	26.070870125220807
11	37.13615010322678	21.21911250178581	22.8605443987215	18.784192996265908
12	25.360107032045743	23.852447719350142	28.919998251989202	21.86744699661491
13	29.50937247505735	19.644600138735267	25.54196965978481	25.30405772642257
14	23.605845166184725	20.519937628551467	25.45211175986432	30.422105445399488
15	24.82966265388359	27.922044081775375	22.805563540775335	24.4427297235657
16	25.13160113831133	25.84262548963468	24.738436609293522	24.28733676276048
17	23.315420822593296	26.243247786618852	25.96329709738877	24.47803429339908
18	24.491353257501817	24.475441413599246	27.684703451413405	23.348501877485525
19	25.4002840662373	24.912665039962572	26.224244240935235	23.462806652864895
20	25.65261190434036	24.17218914091442	25.909443374935847	24.26575557980937
21	26.908026991956646	24.09050059725643	24.947726055395812	24.05374635539111
22	25.657058366404062	24.670903529841535	25.55609529524415	24.11594280851026
23	23.55730791233161	24.50549678362098	26.55550768137761	25.3816876226698
24	24.52202104328902	25.27822572059649	26.141058843437165	24.05869439267732
25	24.374404297963924	24.806139436161192	26.18842030886573	24.631035957009153
26	23.754351735062613	25.939226720563834	26.76051602766507	23.545905516708476
27	24.69648852292949	25.23173776429087	25.63593414523727	24.43583956754237
28	23.593952575769293	25.177076084727585	26.499894107324746	24.729077232178376
29	23.805049471055554	25.247971599876962	26.429293005696497	24.51768592337099
30	23.553631496500614	25.43201687056414	26.822079193942017	24.192272438993236
31	24.65353940280194	25.204234565099853	25.347945328279646	24.79428070381856
32	23.73698889861286	25.574763197674617	25.46887028510406	25.21937761860847
33	23.506400221427604	25.095053625596854	26.41325383156038	24.985292321415166
34	24.48151240257521	25.20162939637252	26.496517641521596	23.82034055953068
35	25.20047269587377	25.065712907298156	26.346743379820325	23.38707101700775
36	23.698035735097676	25.910691392557126	25.885420869081877	24.505852003263314
37	24.74321477397521	25.75096931800354	25.501692104260513	24.00412380376073
38	23.910310283389922	25.713909525890564	25.973767662845553	24.40201252787396
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1118.0
1	833.0
2	548.0
3	548.0
4	1219.0
5	1890.0
6	1890.0
7	2274.5
8	2659.0
9	2495.0
10	2331.0
11	2331.0
12	3018.0
13	3705.0
14	4744.5
15	5784.0
16	5784.0
17	8492.5
18	11201.0
19	11201.0
20	13292.5
21	15384.0
22	15512.0
23	15640.0
24	15640.0
25	18323.0
26	21006.0
27	21006.0
28	27307.5
29	33609.0
30	41966.5
31	50324.0
32	50324.0
33	62748.5
34	75173.0
35	75173.0
36	81707.0
37	88241.0
38	97603.0
39	106965.0
40	106965.0
41	110352.0
42	113739.0
43	125666.0
44	137593.0
45	137593.0
46	139739.0
47	141885.0
48	141885.0
49	149384.5
50	156884.0
51	156641.5
52	156399.0
53	156399.0
54	148077.5
55	139756.0
56	139756.0
57	135654.5
58	131553.0
59	117698.5
60	103844.0
61	103844.0
62	99776.5
63	95709.0
64	78966.0
65	62223.0
66	62223.0
67	52011.5
68	41800.0
69	41800.0
70	33657.5
71	25515.0
72	19988.0
73	14461.0
74	14461.0
75	10774.5
76	7088.0
77	7088.0
78	7181.5
79	7275.0
80	5726.5
81	4178.0
82	4178.0
83	4396.5
84	4615.0
85	4615.0
86	3079.5
87	1544.0
88	1379.5
89	1215.0
90	1215.0
91	848.0
92	481.0
93	432.0
94	383.0
95	383.0
96	354.5
97	326.0
98	326.0
99	607.0
100	888.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12045276794858291
2	0.057201058639768904
3	0.015014577586148941
4	0.003977742569464831
5	5.042208890870913E-4
6	2.2409817292759612E-4
7	0.0
8	2.8012271615949515E-4
9	1.680736296956971E-4
10	2.8012271615949515E-4
11	0.001512662667261274
12	0.0025771289886673556
13	0.01333384128919197
14	0.007899460595697764
15	0.021569449144281126
16	0.010252491411437522
17	0.01860014835299048
18	0.005490405236726105
19	0.007675362422770168
20	0.005826552496117499
21	0.005826552496117499
22	0.006778969731059782
23	0.009019951460335743
24	0.012885644943336777
25	0.015294700302308435
26	0.01675133842633781
27	0.008739828744176248
28	0.005938601582581297
29	0.008459706028016755
30	0.0026891780751311534
31	0.0054343806934942064
32	0.006162699755508893
33	0.008403681484784854
34	0.012437448597481585
35	0.015406749388772234
36	0.013669988548583364
37	0.009636221435886633
38	0.006274748841972692
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1784932.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.29681460847046
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.20173785057167	36.320043912038564
2	14.258190981836858	13.772504130038804
3	4.875914893298123	7.064734729448984
4	2.1273233127908733	4.109717586005521
5	1.0423972624917355	2.5172233667470234
6	0.5996146387709467	1.737568622714724
7	0.3946559138954946	1.3342436455283027
8	0.2530074650902795	0.9775563708819432
9	0.18369679065563888	0.7984772858219759
>10	0.8654286640739722	7.489684901897668
>50	0.08166637941124326	2.783083312472618
>100	0.09369764969718226	9.934304170607719
>500	0.01534462594317775	5.1613444957448325
>1k	0.0070910771404079	5.2959770364633725
>5k	2.324943324723902E-4	0.7035364335880007
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	7527	0.421696736906504	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	5004	0.28034681433242276	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2666	0.14936143225624282	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2491	0.13955713719066049	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2405	0.13473902647271718	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2402	0.13457095284302148	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2378	0.1332263638054559	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2354	0.13188177476789031	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2354	0.13188177476789031	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2265	0.1268955904202513	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2195	0.12297387239401839	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2193	0.12286182330755457	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2126	0.11910817891101734	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2075	0.11625092720619049	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1967	0.11020027653714538	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1953	0.1094159329318988	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1867	0.1045978222139555	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1788	0.10017188329863545	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.680736296956971E-4
2	0.0	0.0	0.0	0.0	2.2409817292759612E-4
3	0.0	0.0	0.0	0.0	2.2409817292759612E-4
4	0.0	0.0	0.0	0.0	3.361472593913942E-4
5	0.0	0.0	0.0	0.0	3.361472593913942E-4
6	0.0	0.0	0.0	0.0	5.042208890870913E-4
7	0.0	0.0	0.0	0.0	5.042208890870913E-4
8	0.0	0.0	0.0	0.0	5.042208890870913E-4
9	0.0	0.0	0.0	5.602454323189903E-5	5.602454323189903E-4
10	0.0	0.0	0.0	5.602454323189903E-5	5.602454323189903E-4
11	0.0	0.0	0.0	5.602454323189903E-5	5.602454323189903E-4
12	0.0	0.0	0.0	5.602454323189903E-5	0.0010084417781741825
13	0.0	0.0	0.0	5.602454323189903E-5	0.0010084417781741825
14	0.0	0.0	0.0	5.602454323189903E-5	0.0010644663214060817
15	0.0	0.0	0.0	1.1204908646379806E-4	0.0012885644943336778
16	0.0	0.0	0.0	1.1204908646379806E-4	0.001512662667261274
17	0.0	0.0	0.0	1.1204908646379806E-4	0.001512662667261274
18	0.0	0.0	0.0	1.680736296956971E-4	0.001624711753725072
19	0.0	0.0	0.0	1.680736296956971E-4	0.001624711753725072
20	0.0	0.0	0.0	2.2409817292759612E-4	0.001680736296956971
21	0.0	0.0	5.602454323189903E-5	7.283190620146874E-4	0.001848809926652668
22	0.0	0.0	5.602454323189903E-5	0.0012325399511017787	0.001848809926652668
23	0.0	0.0	5.602454323189903E-5	0.0029132762480587496	0.001848809926652668
24	0.0	0.0	5.602454323189903E-5	0.004594012545015721	0.001848809926652668
25	0.0	0.0	5.602454323189903E-5	0.006218724298740793	0.001848809926652668
26	0.0	0.0	5.602454323189903E-5	0.007451264249842571	0.001960859013116466
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACG	920	0.0	17.911161	4
CGGTAAC	45	0.00885321	17.777403	29
TCTAGCG	580	0.0	17.378946	28
AGGACGT	940	0.0	17.359392	5
GGACGTG	905	0.0	16.970592	6
TTAGGAC	545	0.0	16.734062	3
TAGCGGC	610	0.0	16.524242	30
CTAGCGG	615	0.0	16.3899	29
GACGTGA	490	0.0	16.325274	7
TGTAGGA	1025	0.0	16.080866	2
GTATTAG	520	0.0	16.012226	1
ATCGTTT	495	0.0	15.838051	29
GTCCTAC	745	0.0	15.689831	1
GTAGGAC	995	0.0	15.1156845	3
CATCGTT	510	0.0	15.058507	28
CTGTAGG	1100	0.0	14.993265	1
TCTAGTA	75	6.2384567E-4	14.935948	2
GTACGAA	75	6.2464183E-4	14.933437	19
AGCGGCG	700	0.0	14.856831	31
AAGACGG	630	0.0	14.728199	5
>>END_MODULE