FastQCFastQC Report
Thu 2 Feb 2017
SRR4062874_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062874_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1784932
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT79430.445002946890974No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT58030.32511042437471005No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT33900.1899232015561377No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC26470.14829696593483674No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG25570.1432547570439658No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25470.1426945116116468No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT25210.14123787348761746No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA24960.13983725990681997No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC24450.13698000820199313No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA24040.13468300192948526No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA23420.13120948024910753No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG23110.12947271940891866No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC21920.12280579876432268No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG21800.12213350424553988No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG21360.11966842434333633No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG20280.11361777367429123No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG19460.1090237611292755No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC19440.10891171204281172No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT18910.10594241125152107No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT18250.10224479139821573No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG17860.10005983421217167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG552.2188487E-623.2737265
ATCGTTT4500.018.84419829
CATCGTT4650.017.89223928
TACCGTC4900.017.3068667
CGCGTAA2050.017.17146910
ACCGTCG4850.017.1553768
AATCCCG1800.016.88866619
CGTCGTA5150.016.46672610
TCTAGCG5350.016.44838128
TCGCGTA2150.016.3727959
GCGTAAC2150.016.37233711
CGGCATC5200.016.30747825
TAACGAA2850.016.28048913
ATAACGA2950.016.27097312
CCGTCGT5150.016.1560339
GGCATCG5300.015.99978926
AACCGCG3650.015.78157
CGCAAGA7200.015.7775712
TTAGGAC5900.015.7286073
ATACCGT5400.015.7043776
Produced by FastQC (version 0.11.5)