##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062874_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1784932
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.160165765418515	32.0	32.0	32.0	32.0	32.0
2	31.270919564442792	32.0	32.0	32.0	32.0	32.0
3	31.357764329397423	32.0	32.0	32.0	32.0	32.0
4	31.464492204745056	32.0	32.0	32.0	32.0	32.0
5	31.382907023909034	32.0	32.0	32.0	32.0	32.0
6	34.8993849625644	36.0	36.0	36.0	36.0	36.0
7	34.924473313269075	36.0	36.0	36.0	36.0	36.0
8	34.864057006093226	36.0	36.0	36.0	32.0	36.0
9	34.97652963810386	36.0	36.0	36.0	36.0	36.0
10	34.82167780061089	36.0	36.0	36.0	32.0	36.0
11	35.006930235997785	36.0	36.0	36.0	36.0	36.0
12	34.89436068152737	36.0	36.0	36.0	32.0	36.0
13	34.94572734423496	36.0	36.0	36.0	36.0	36.0
14	34.894549484238055	36.0	36.0	36.0	32.0	36.0
15	34.85833297851123	36.0	36.0	36.0	32.0	36.0
16	34.869637050599124	36.0	36.0	36.0	32.0	36.0
17	34.834868779314846	36.0	36.0	36.0	32.0	36.0
18	34.83322557946185	36.0	36.0	36.0	32.0	36.0
19	34.82280277343899	36.0	36.0	36.0	32.0	36.0
20	34.80542339988302	36.0	36.0	36.0	32.0	36.0
21	34.79112089424135	36.0	36.0	36.0	32.0	36.0
22	34.77670073705889	36.0	36.0	36.0	32.0	36.0
23	34.72134960883664	36.0	36.0	36.0	32.0	36.0
24	34.69758399759767	36.0	36.0	36.0	32.0	36.0
25	34.67766727247873	36.0	36.0	36.0	32.0	36.0
26	34.61738542420664	36.0	36.0	36.0	32.0	36.0
27	34.595640057996604	36.0	36.0	36.0	32.0	36.0
28	34.55718649225853	36.0	36.0	36.0	32.0	36.0
29	34.51439438589257	36.0	36.0	36.0	32.0	36.0
30	34.48687737123879	36.0	36.0	36.0	32.0	36.0
31	34.48444254458993	36.0	36.0	36.0	32.0	36.0
32	34.44842772721874	36.0	36.0	36.0	32.0	36.0
33	34.404710655644024	36.0	36.0	36.0	32.0	36.0
34	34.39325755827113	36.0	36.0	36.0	32.0	36.0
35	34.34475487021354	36.0	36.0	36.0	32.0	36.0
36	34.314032131196036	36.0	36.0	36.0	32.0	36.0
37	34.29699226637205	36.0	36.0	36.0	32.0	36.0
38	33.86322896334426	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	4.0
20	10.0
21	42.0
22	150.0
23	414.0
24	1188.0
25	2926.0
26	6224.0
27	12312.0
28	21536.0
29	33834.0
30	51948.0
31	74885.0
32	107738.0
33	171319.0
34	410954.0
35	889447.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.72016172002333	18.993362329835115	12.202622907834042	26.083853042307513
2	15.335150022521866	21.214757761079976	37.60669874258515	25.84339347381301
3	17.64817931439405	25.62747488419727	29.04609251220775	27.67825328920093
4	11.693617288287335	16.45525793434032	36.74338111669302	35.10774366067932
5	13.768887295300095	37.467295636243435	33.190153115248215	15.57366395320825
6	33.2582043787687	36.56920071420128	16.86423732906202	13.308357577968
7	29.348420436948576	31.12765585537535	21.352449740157002	18.171473967519063
8	27.660046512331483	33.29183714745283	19.65658638032431	19.391529959891376
9	27.623775745001993	14.024285364518581	18.924722989633366	39.42721590084606
10	15.784617194656327	26.602723378984432	31.510183958339756	26.102475468019488
11	37.185533798358406	21.18138779169118	22.95901616382329	18.674062246127125
12	25.14053835405693	24.04088245872929	28.83997171865213	21.978607468561652
13	29.488999568610534	19.650182360092554	25.59248822082659	25.268329850470327
14	23.515125506181747	20.460387286462453	25.44287401424816	30.581613193107636
15	24.713658559541766	28.035073605044897	22.781988333449117	24.46927950196422
16	25.043321002324458	25.982292872945788	24.616021571702213	24.35836455302754
17	23.273099479419944	26.419157704607233	25.84580252917198	24.461940286800843
18	24.47364941633631	24.400593411961914	27.67461169389086	23.45114547781092
19	25.353794990509442	24.928344609206402	26.225424834111326	23.49243556617283
20	25.66926919344826	24.053409317553832	25.900594532452775	24.376726956545124
21	26.754733661675058	24.157543521338944	25.051976857342783	24.03574595964321
22	25.513128791460964	24.75461250064428	25.615653705575337	24.116605002319417
23	23.43327013750695	24.440761756216496	26.621970185912257	25.503997920364295
24	24.523959456158554	25.268021414821405	26.14547781091941	24.06254131810063
25	24.353853850769415	24.76664337909239	26.243396796175084	24.63610597396311
26	23.757626349492696	25.86342321547624	26.76385068322007	23.615099751810995
27	24.726726131150166	25.13172778107389	25.649114727684154	24.49243136009179
28	23.625605905435055	25.096642337075025	26.57333724758142	24.704414509908503
29	23.860236692490247	25.09232844724617	26.532159208305973	24.51527565195761
30	23.58655679880242	25.19199610965572	27.011225077481942	24.21022201405992
31	24.618528885134	24.982184195252255	25.537779590483	24.861507329130745
32	23.870321110271988	25.436039019973872	25.567136451136513	25.12650341861763
33	23.45377863134282	24.927504241057925	26.56089979898394	25.057817328615318
34	24.480820557869993	25.00767536242277	26.684489941353505	23.827014138353732
35	25.187458121653933	24.972043752927284	26.361956645967467	23.478541479451316
36	23.740400194517214	25.668484849843022	26.091414126700624	24.49970082893914
37	24.788339275669884	25.610835594857395	25.55637973883599	24.044445390636728
38	23.990366003353635	25.372885980338715	26.06249324202811	24.574254774279535
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	820.0
1	597.5
2	375.0
3	375.0
4	1115.0
5	1855.0
6	1855.0
7	2165.0
8	2475.0
9	2489.5
10	2504.0
11	2504.0
12	3183.0
13	3862.0
14	4895.0
15	5928.0
16	5928.0
17	8876.5
18	11825.0
19	11825.0
20	13497.5
21	15170.0
22	15694.5
23	16219.0
24	16219.0
25	18526.0
26	20833.0
27	20833.0
28	26900.5
29	32968.0
30	41600.0
31	50232.0
32	50232.0
33	63604.0
34	76976.0
35	76976.0
36	82912.0
37	88848.0
38	97997.0
39	107146.0
40	107146.0
41	110348.5
42	113551.0
43	126205.0
44	138859.0
45	138859.0
46	140760.5
47	142662.0
48	142662.0
49	150455.5
50	158249.0
51	157103.5
52	155958.0
53	155958.0
54	147614.5
55	139271.0
56	139271.0
57	135644.5
58	132018.0
59	117765.0
60	103512.0
61	103512.0
62	99931.0
63	96350.0
64	78950.0
65	61550.0
66	61550.0
67	51576.5
68	41603.0
69	41603.0
70	33488.0
71	25373.0
72	19623.5
73	13874.0
74	13874.0
75	10421.0
76	6968.0
77	6968.0
78	7158.0
79	7348.0
80	5680.0
81	4012.0
82	4012.0
83	3937.0
84	3862.0
85	3862.0
86	2414.5
87	967.0
88	820.5
89	674.0
90	674.0
91	404.5
92	135.0
93	93.5
94	52.0
95	52.0
96	38.0
97	24.0
98	24.0
99	24.0
100	24.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.006666920644595985
2	0.0
3	0.0
4	5.602454323189903E-5
5	1.1204908646379806E-4
6	5.602454323189903E-5
7	4.4819634585519224E-4
8	0.001624711753725072
9	0.003977742569464831
10	9.524172349422835E-4
11	0.004594012545015721
12	5.602454323189903E-4
13	1.1204908646379806E-4
14	0.0
15	0.0
16	5.602454323189903E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	1.680736296956971E-4
22	0.0
23	2.2409817292759612E-4
24	0.0
25	1.680736296956971E-4
26	1.1204908646379806E-4
27	5.602454323189903E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.680736296956971E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1784932.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.578689584298154
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.64532342936863	33.110786463381345
2	15.578709651681521	14.201143426758033
3	5.4071428425877315	7.393514554807973
4	2.4040508661157336	4.382939526862086
5	1.1959744185774577	2.725547338755171
6	0.6751600867537079	1.846374720831304
7	0.44780751898904775	1.4287335931061553
8	0.27353768749182245	0.9973991478237225
9	0.197626524188881	0.8106802199665917
>10	0.9590164855707103	7.821392899957368
>50	0.08850386581833011	2.868157674669347
>100	0.10196452802700193	10.327738993388403
>500	0.017034946502239943	5.554797873650048
>1k	0.007900265044517074	5.757401233787534
>5k	2.4688328264115857E-4	0.7733923322548953
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	7943	0.445002946890974	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	5803	0.32511042437471005	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	3390	0.1899232015561377	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2647	0.14829696593483674	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2557	0.1432547570439658	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2547	0.1426945116116468	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2521	0.14123787348761746	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	2496	0.13983725990681997	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2445	0.13698000820199313	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2404	0.13468300192948526	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2342	0.13120948024910753	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2311	0.12947271940891866	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2192	0.12280579876432268	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2180	0.12213350424553988	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2136	0.11966842434333633	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2028	0.11361777367429123	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1946	0.1090237611292755	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1944	0.10891171204281172	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1891	0.10594241125152107	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1825	0.10224479139821573	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1786	0.10005983421217167	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1204908646379806E-4
2	0.0	0.0	0.0	0.0	1.1204908646379806E-4
3	0.0	0.0	0.0	0.0	1.1204908646379806E-4
4	0.0	0.0	0.0	0.0	1.1204908646379806E-4
5	0.0	0.0	0.0	0.0	1.1204908646379806E-4
6	0.0	0.0	0.0	0.0	1.680736296956971E-4
7	0.0	0.0	0.0	0.0	1.680736296956971E-4
8	0.0	0.0	0.0	0.0	1.680736296956971E-4
9	0.0	0.0	0.0	0.0	2.2409817292759612E-4
10	0.0	0.0	0.0	0.0	2.2409817292759612E-4
11	0.0	0.0	0.0	0.0	2.2409817292759612E-4
12	0.0	0.0	0.0	0.0	6.722945187827884E-4
13	0.0	0.0	0.0	0.0	9.524172349422835E-4
14	0.0	0.0	0.0	0.0	9.524172349422835E-4
15	0.0	0.0	0.0	5.602454323189903E-5	0.0012325399511017787
16	0.0	0.0	0.0	5.602454323189903E-5	0.0014006135807974759
17	0.0	0.0	0.0	5.602454323189903E-5	0.0014006135807974759
18	0.0	0.0	0.0	1.1204908646379806E-4	0.001680736296956971
19	0.0	0.0	0.0	1.1204908646379806E-4	0.001680736296956971
20	0.0	0.0	0.0	1.680736296956971E-4	0.001848809926652668
21	0.0	0.0	0.0	7.283190620146874E-4	0.001848809926652668
22	0.0	0.0	0.0	0.0012325399511017787	0.001904834469884567
23	0.0	0.0	0.0	0.0028012271615949517	0.001960859013116466
24	0.0	0.0	0.0	0.004650037088247619	0.001960859013116466
25	0.0	0.0	0.0	0.006274748841972691	0.001960859013116466
26	0.0	0.0	0.0	0.007395239706610672	0.0023530308157397595
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACACCG	55	2.2188487E-6	23.273726	5
ATCGTTT	450	0.0	18.844198	29
CATCGTT	465	0.0	17.892239	28
TACCGTC	490	0.0	17.306866	7
CGCGTAA	205	0.0	17.171469	10
ACCGTCG	485	0.0	17.155376	8
AATCCCG	180	0.0	16.888666	19
CGTCGTA	515	0.0	16.466726	10
TCTAGCG	535	0.0	16.448381	28
TCGCGTA	215	0.0	16.372795	9
GCGTAAC	215	0.0	16.372337	11
CGGCATC	520	0.0	16.307478	25
TAACGAA	285	0.0	16.280489	13
ATAACGA	295	0.0	16.270973	12
CCGTCGT	515	0.0	16.156033	9
GGCATCG	530	0.0	15.999789	26
AACCGCG	365	0.0	15.7815	7
CGCAAGA	720	0.0	15.777571	2
TTAGGAC	590	0.0	15.728607	3
ATACCGT	540	0.0	15.704377	6
>>END_MODULE