FastQCFastQC Report
Thu 2 Feb 2017
SRR4062873_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062873_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1836551
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT127090.6920036524986237No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA86750.47235279608352826No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT84060.45770577566318604No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC69900.380604731368745No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT45720.24894489725578No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA37990.20685513225606042No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT37620.20484048632463786No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG34000.18512962613072004No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT22180.1207698561052756No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC22150.12060650643516024No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA21820.11880966006389149No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG20350.11080552622823978No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA20160.10977097831750927No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG19650.10699403392554849No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC19410.10568723656462575No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG18840.10258359283243426No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA18550.10100454602131931No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG4400.020.36308329
TAGCGGC4650.019.26829130
TCTAGCG4650.019.26724228
ACGAACG2600.018.46304515
AACCCGT1750.018.28521529
CGAACGA2650.018.11468716
TAATACC1201.6661943E-917.3290864
GATATAC7950.016.7180611
ACGAGAC2900.016.55037319
TAACGAA3000.016.53513513
AGCGGCG5550.016.43198631
GTCCTAC5000.016.0130831
GTCCTAA4000.016.0130831
ACCCGTT1900.015.99956530
TAACCCG2000.015.998692528
GTAGAAC11800.015.9965143
ATACACT8950.015.9067164
GAACAGT6850.015.8788876
TGTAGAA12700.015.8753132
GATAACG3150.015.74774711
Produced by FastQC (version 0.11.5)