##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062873_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1836551
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.07830438686429	32.0	32.0	32.0	32.0	32.0
2	30.718526193936352	32.0	32.0	32.0	32.0	32.0
3	30.790660863760387	32.0	32.0	32.0	32.0	32.0
4	30.83121024137092	32.0	32.0	32.0	32.0	32.0
5	30.772575877283018	32.0	32.0	32.0	32.0	32.0
6	34.40792441919663	36.0	36.0	36.0	32.0	36.0
7	34.28438850867741	36.0	36.0	36.0	32.0	36.0
8	34.262716363444305	36.0	36.0	36.0	32.0	36.0
9	34.37107654511092	36.0	36.0	36.0	32.0	36.0
10	34.11791341487386	36.0	36.0	36.0	32.0	36.0
11	34.43140920126912	36.0	36.0	36.0	32.0	36.0
12	34.234151406631234	36.0	36.0	36.0	32.0	36.0
13	34.31580010574169	36.0	36.0	36.0	32.0	36.0
14	34.21824986074441	36.0	36.0	36.0	32.0	36.0
15	34.1805607358576	36.0	36.0	36.0	32.0	36.0
16	34.17931873386582	36.0	36.0	36.0	32.0	36.0
17	34.096531487554664	36.0	36.0	36.0	32.0	36.0
18	34.13884830859584	36.0	36.0	36.0	32.0	36.0
19	34.11905958505917	36.0	36.0	36.0	32.0	36.0
20	34.06678660162446	36.0	36.0	36.0	32.0	36.0
21	34.0121793514038	36.0	36.0	36.0	32.0	36.0
22	34.00820233143539	36.0	36.0	36.0	32.0	36.0
23	33.93986663043934	36.0	36.0	36.0	32.0	36.0
24	33.929920813524916	36.0	36.0	36.0	32.0	36.0
25	33.89171768167614	36.0	36.0	36.0	32.0	36.0
26	33.85123582192926	36.0	36.0	36.0	32.0	36.0
27	33.845735294037574	36.0	36.0	36.0	32.0	36.0
28	33.81874502804442	36.0	36.0	36.0	32.0	36.0
29	33.77067775411628	36.0	36.0	36.0	32.0	36.0
30	33.72174853842883	36.0	36.0	36.0	27.0	36.0
31	33.754273091245494	36.0	36.0	36.0	27.0	36.0
32	33.69401176444324	36.0	36.0	36.0	27.0	36.0
33	33.62829238066354	36.0	36.0	36.0	27.0	36.0
34	33.60722898520106	36.0	36.0	36.0	27.0	36.0
35	33.528953456778495	36.0	36.0	36.0	21.0	36.0
36	33.48367183922472	36.0	36.0	36.0	21.0	36.0
37	33.45970081963419	36.0	36.0	36.0	21.0	36.0
38	32.78551807164625	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	2.0
8	4.0
9	6.0
10	12.0
11	25.0
12	8.0
13	5.0
14	461.0
15	853.0
16	1150.0
17	1455.0
18	1933.0
19	2424.0
20	2953.0
21	3792.0
22	5098.0
23	7045.0
24	10039.0
25	14260.0
26	20374.0
27	29233.0
28	39817.0
29	53909.0
30	72277.0
31	95338.0
32	125865.0
33	184633.0
34	391086.0
35	772492.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.54380241701593	17.145352412436598	12.02609347946496	24.28475169108251
2	15.452602492251552	20.30815094722012	39.03506836448784	25.204178196040488
3	19.355072345384546	25.70022817249623	28.120490325812895	26.824209156306328
4	11.984424653088606	15.796420567037591	36.136013660827274	36.08314111904653
5	13.676037871049381	37.64165162032248	33.32137248795022	15.360938020677924
6	33.0100988760937	35.80165103855563	17.58049507198587	13.607755013364798
7	29.07593041300264	31.281369959979312	21.041953663118345	18.600745963899705
8	28.280681082487952	32.92031186786943	19.765570932842994	19.033436116799624
9	27.79290277193953	14.533352790125823	18.780723400640767	38.89302103729388
10	15.940039585199383	27.35190262149852	31.45918014532723	25.248877647974865
11	36.935072855182625	21.952170409252282	21.936270772983686	19.176485962581406
12	24.651932456192412	24.32903511413305	29.41366089898323	21.60537153069131
13	29.90415352862052	20.15057698486606	25.637351805562364	24.307917680951057
14	23.963702284235904	20.318465406462426	25.843226497506766	29.874605811794908
15	25.050215567479484	27.133235734312812	24.503563017814002	23.31298568039371
16	24.917907269794558	26.340139996013807	25.418141976041447	23.323810758150188
17	22.81783831062807	26.007958007513597	26.782028366059627	24.392175315798703
18	24.03389376425309	25.06534367505897	28.52768720418374	22.373075356504202
19	24.393353103834336	26.03895870521007	26.811827014287694	22.755861176667906
20	24.669892101315313	24.43236797181511	27.62424140118544	23.273498525684133
21	26.30491592069574	24.89668763317344	25.308902479333444	23.489493966797372
22	24.441756864265525	25.225233074370884	26.502710964909653	23.830299096453935
23	23.297884911431584	25.070124489688116	26.699256198482118	24.932734400398175
24	23.409235347070233	26.625481750787326	26.545591582070404	23.419691320072037
25	24.53054398136172	24.96164677345483	26.405699531810168	24.10210971337328
26	22.776443780501683	26.206629128364543	27.643098211548068	23.373828879585705
27	24.164356438878478	25.851026928243808	26.813671756519142	23.17094487635857
28	23.18024645643994	25.715219202474366	27.269702629558434	23.834831711527258
29	22.709690769930326	26.002063893570615	27.51344389776375	23.774801438735306
30	23.508934901383387	26.109906529028493	27.356131697908964	23.025026871679156
31	23.271171092189142	26.260502028118673	26.34675628978165	24.121570589910537
32	22.829858464058407	26.63023354083806	26.261092209642218	24.278815785461312
33	22.747066943658638	25.962007084532466	26.944039817141736	24.34688615466716
34	23.254698229621038	26.632958128440965	27.304406070804266	22.807937571133728
35	24.270892954293334	26.27159765021487	27.01544939613441	22.442059999357387
36	22.891465877232537	27.394006922797086	26.26278963889488	23.451737561075493
37	23.90174757619916	27.083346947132714	26.38593923235597	22.628966244312153
38	23.07296471072553	27.10891069699666	26.267546348279424	23.550578243998384
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	691.0
1	762.5
2	834.0
3	834.0
4	1936.5
5	3039.0
6	3039.0
7	3687.0
8	4335.0
9	4252.5
10	4170.0
11	4170.0
12	5184.5
13	6199.0
14	7784.0
15	9369.0
16	9369.0
17	13330.5
18	17292.0
19	17292.0
20	19998.5
21	22705.0
22	21918.0
23	21131.0
24	21131.0
25	24619.5
26	28108.0
27	28108.0
28	35821.0
29	43534.0
30	50902.5
31	58271.0
32	58271.0
33	66219.0
34	74167.0
35	74167.0
36	80094.5
37	86022.0
38	96880.0
39	107738.0
40	107738.0
41	111509.0
42	115280.0
43	128177.0
44	141074.0
45	141074.0
46	155618.0
47	170162.0
48	170162.0
49	167752.5
50	165343.0
51	157761.0
52	150179.0
53	150179.0
54	141101.0
55	132023.0
56	132023.0
57	128053.5
58	124084.0
59	111217.0
60	98350.0
61	98350.0
62	93361.5
63	88373.0
64	73815.0
65	59257.0
66	59257.0
67	49667.5
68	40078.0
69	40078.0
70	32264.5
71	24451.0
72	19151.5
73	13852.0
74	13852.0
75	10616.0
76	7380.0
77	7380.0
78	7098.5
79	6817.0
80	5420.0
81	4023.0
82	4023.0
83	3878.0
84	3733.0
85	3733.0
86	2550.5
87	1368.0
88	1145.5
89	923.0
90	923.0
91	696.0
92	469.0
93	420.5
94	372.0
95	372.0
96	345.5
97	319.0
98	319.0
99	677.5
100	1036.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12104210555546784
2	0.055647787619292906
3	0.012033425698496802
4	0.003212543512268377
5	1.633496701153412E-4
6	5.444989003844707E-4
7	5.4449890038447064E-5
8	2.1779956015378826E-4
9	1.633496701153412E-4
10	3.266993402306824E-4
11	0.0016879465911918592
12	0.0029402940620761415
13	0.015300419100803625
14	0.009909879986997366
15	0.02542809864795478
16	0.011597826578189226
17	0.022487804585878637
18	0.005826138234113836
19	0.007895234055574825
20	0.0057716883440753885
21	0.007078485704998119
22	0.006533986804613649
23	0.00947428086668979
24	0.01524596921076518
25	0.01796846371268753
26	0.02079985799468678
27	0.008276383285843954
28	0.006588436694652095
29	0.011761176248304566
30	0.002450245051730118
31	0.006533986804613649
32	0.006915136034882778
33	0.008494182845997744
34	0.012033425698496802
35	0.016226067231457225
36	0.016280517121495672
37	0.009746530316882026
38	0.007024035814959672
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1836551.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.866783860581926
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.40714064089074	31.324016045961294
2	14.988719831228684	13.150138663666533
3	5.841963498290734	7.688044503027861
4	2.807065193473422	4.925476884986447
5	1.4933978488038007	3.2755280325667164
6	0.8963610818148529	2.3592286702205723
7	0.5686259656675482	1.7460654633417112
8	0.39438373474041616	1.3840276839989527
9	0.25902068572803105	1.022616399262613
>10	1.1187250081644273	8.503918729542285
>50	0.09659882556711571	2.9974558356595296
>100	0.10558613448983342	10.057044876915183
>500	0.014442999623801857	4.284011651821549
>1k	0.007470517046794065	5.273579236957238
>5k	3.7352585233970325E-4	1.3147080992273512
>10k+	1.2450861744656776E-4	0.6941392228441031
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	12709	0.6920036524986237	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	8675	0.47235279608352826	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	8406	0.45770577566318604	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	6990	0.380604731368745	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	4572	0.24894489725578	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	3799	0.20685513225606042	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3762	0.20484048632463786	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	3400	0.18512962613072004	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2218	0.1207698561052756	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2215	0.12060650643516024	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	2182	0.11880966006389149	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	2035	0.11080552622823978	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2016	0.10977097831750927	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1965	0.10699403392554849	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1941	0.10568723656462575	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1884	0.10258359283243426	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	1855	0.10100454602131931	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	5.444989003844707E-5	0.0
8	0.0	0.0	0.0	5.444989003844707E-5	0.0
9	0.0	0.0	0.0	5.444989003844707E-5	0.0
10	0.0	0.0	0.0	5.444989003844707E-5	0.0
11	0.0	0.0	0.0	5.444989003844707E-5	0.0
12	0.0	0.0	0.0	5.444989003844707E-5	0.0
13	0.0	0.0	0.0	5.444989003844707E-5	0.0
14	0.0	0.0	0.0	1.633496701153412E-4	0.0
15	0.0	0.0	0.0	2.1779956015378828E-4	0.0
16	0.0	0.0	0.0	5.444989003844707E-4	0.0
17	0.0	0.0	0.0	7.078485704998119E-4	0.0
18	0.0	0.0	0.0	8.711982406151531E-4	5.444989003844707E-5
19	0.0	0.0	0.0	9.800980206920472E-4	5.444989003844707E-5
20	0.0	0.0	0.0	0.0012523474708842825	5.444989003844707E-5
21	0.0	0.0	0.0	0.0019057461513456474	5.444989003844707E-5
22	0.0	0.0	0.0	0.0028313942819992474	5.444989003844707E-5
23	0.0	0.0	0.0	0.0049004901034602364	5.444989003844707E-5
24	0.0	0.0	0.0	0.006969585924921225	5.444989003844707E-5
25	0.0	0.0	0.0	0.008330833175882402	5.444989003844707E-5
26	0.0	0.0	0.0	0.01094442789772786	5.444989003844707E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	440	0.0	20.363083	29
TAGCGGC	465	0.0	19.268291	30
TCTAGCG	465	0.0	19.267242	28
ACGAACG	260	0.0	18.463045	15
AACCCGT	175	0.0	18.285215	29
CGAACGA	265	0.0	18.114687	16
TAATACC	120	1.6661943E-9	17.329086	4
GATATAC	795	0.0	16.718061	1
ACGAGAC	290	0.0	16.550373	19
TAACGAA	300	0.0	16.535135	13
AGCGGCG	555	0.0	16.431986	31
GTCCTAC	500	0.0	16.013083	1
GTCCTAA	400	0.0	16.013083	1
ACCCGTT	190	0.0	15.999565	30
TAACCCG	200	0.0	15.9986925	28
GTAGAAC	1180	0.0	15.996514	3
ATACACT	895	0.0	15.906716	4
GAACAGT	685	0.0	15.878887	6
TGTAGAA	1270	0.0	15.875313	2
GATAACG	315	0.0	15.747747	11
>>END_MODULE