FastQCFastQC Report
Thu 2 Feb 2017
SRR4062873_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062873_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1836551
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT144940.7891967062172519No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT104050.5665511058500416No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA87540.4766543373965656No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC85000.4628240653268001No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT62770.34178195977133224No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT45190.2460590530837423No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG36830.20053894501160055No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA35430.19291596040621795No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC24510.13345668048423376No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT22160.12066095632519869No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC21630.117775112153161No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG21250.11570601633170002No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC21100.1148892679811233No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG20900.11380027018035437No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA20290.1104788268880091No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG19290.10503383788416439No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG19170.10438043920370302No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC18530.10089564624124242No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA18410.10024224756078105No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCC401.5933772E-424.001514
GTATTAC1208.185452E-1118.6683481
CTAGCGG5250.018.58962229
TAGCGGC5400.018.36952830
TCTAGCG5600.017.4277728
ACACCGT753.2321346E-517.068676
GATATAC7900.017.0141931
AGCGGCG5850.016.95648631
CGGTCCA6100.016.78794110
CATCGTT4200.016.76113528
TAGGACG5000.016.6410484
TACCGTC4300.016.3740147
ATCGTTT4300.016.3713429
ATACCGT4500.016.3574756
GCGCAAG6000.016.2681351
ACCGTCG4250.016.1901368
CCGGTCC6400.016.0010079
TCTTGCG6300.016.0001372
CGTTTAT4250.015.81103831
CAAGACG6100.015.7386954
Produced by FastQC (version 0.11.5)