##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062873_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1836551
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.03888865596436	32.0	32.0	32.0	32.0	32.0
2	31.270323013082674	32.0	32.0	32.0	32.0	32.0
3	31.369288955220956	32.0	32.0	32.0	32.0	32.0
4	31.483990915580346	32.0	32.0	32.0	32.0	32.0
5	31.382364007315886	32.0	32.0	32.0	32.0	32.0
6	34.90559369165354	36.0	36.0	36.0	36.0	36.0
7	34.94948302551903	36.0	36.0	36.0	36.0	36.0
8	34.89868291160986	36.0	36.0	36.0	36.0	36.0
9	35.0055168628587	36.0	36.0	36.0	36.0	36.0
10	34.850738694433204	36.0	36.0	36.0	32.0	36.0
11	35.02339820674732	36.0	36.0	36.0	36.0	36.0
12	34.9178552623913	36.0	36.0	36.0	32.0	36.0
13	34.96758380246451	36.0	36.0	36.0	36.0	36.0
14	34.90579025575658	36.0	36.0	36.0	32.0	36.0
15	34.8821720714535	36.0	36.0	36.0	32.0	36.0
16	34.883057971164426	36.0	36.0	36.0	32.0	36.0
17	34.84236103435189	36.0	36.0	36.0	32.0	36.0
18	34.835232999246955	36.0	36.0	36.0	32.0	36.0
19	34.82687820811946	36.0	36.0	36.0	32.0	36.0
20	34.806156213467524	36.0	36.0	36.0	32.0	36.0
21	34.77795062592871	36.0	36.0	36.0	32.0	36.0
22	34.761424539803144	36.0	36.0	36.0	32.0	36.0
23	34.69286178276563	36.0	36.0	36.0	32.0	36.0
24	34.66258492140975	36.0	36.0	36.0	32.0	36.0
25	34.6363144829629	36.0	36.0	36.0	32.0	36.0
26	34.55592520980904	36.0	36.0	36.0	32.0	36.0
27	34.5395842533096	36.0	36.0	36.0	32.0	36.0
28	34.49458577518403	36.0	36.0	36.0	32.0	36.0
29	34.45157417354596	36.0	36.0	36.0	32.0	36.0
30	34.420043331222494	36.0	36.0	36.0	32.0	36.0
31	34.40761786631572	36.0	36.0	36.0	32.0	36.0
32	34.36393925352468	36.0	36.0	36.0	32.0	36.0
33	34.30119610073447	36.0	36.0	36.0	32.0	36.0
34	34.27497684518426	36.0	36.0	36.0	32.0	36.0
35	34.196603306959624	36.0	36.0	36.0	32.0	36.0
36	34.15022888011278	36.0	36.0	36.0	32.0	36.0
37	34.11786822146512	36.0	36.0	36.0	32.0	36.0
38	33.6656264922673	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	7.0
20	9.0
21	43.0
22	163.0
23	493.0
24	1297.0
25	3238.0
26	6775.0
27	13106.0
28	23237.0
29	37097.0
30	56068.0
31	80397.0
32	113513.0
33	179142.0
34	417727.0
35	904239.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.91362496759902	18.337323101520177	12.840373694975508	24.9086782359053
2	14.603678307871656	21.29529754414661	38.170080765521895	25.930943382459837
3	18.440653159100943	26.12200804660475	28.6314945786967	26.805844215597606
4	11.590785959505483	16.638579506627934	36.036143955089585	35.73449057877699
5	13.21973265089434	38.027660559200676	33.13609757425608	15.616509215648907
6	33.18003840903609	36.16526013219373	17.144184167353192	13.51051729141699
7	28.68339334631785	31.43153973189905	21.172776955885148	18.71228996589796
8	28.092142160946576	33.30806802032115	19.56085183309647	19.038937985635798
9	27.407664340294662	14.35012325215807	18.72071502042768	39.521497387119595
10	15.733902667583614	27.447127219742	31.586616289079792	25.232353823594593
11	37.06294049004356	21.72807412566317	21.97904760763844	19.229937776654836
12	24.37431392877056	24.480981669919153	29.461096494284917	21.68360790702537
13	29.967275598268493	20.04573793253655	25.743758677955952	24.243227791239008
14	23.78055387517145	20.19404851811902	25.96110862154114	30.064288985168396
15	25.098459013662023	27.187265695316924	24.298862378447428	23.415412912573625
16	24.857191550901664	26.170904047859278	25.45445239473339	23.517452006505675
17	22.68910582935078	26.018771055091854	26.892365090868697	24.39975802468867
18	24.09298734421206	24.753518960268458	28.752482234362127	22.401011461157356
19	24.395946532385977	25.860158525409858	27.030232212446048	22.713662729758116
20	24.544649182080978	24.26543014596382	27.902791700312164	23.287128971643043
21	26.182530387155474	24.75011557001746	25.627358703742726	23.439995339084337
22	24.214314883885546	25.054259344967466	26.939424464348917	23.79200130679807
23	23.180729924145695	24.64004460544707	27.0682971903251	25.110928280082135
24	23.36150752143556	26.327338581939735	26.801978273404874	23.50917562321983
25	24.399240096507093	24.69506667124046	26.707264548890336	24.19842868336211
26	22.818600092564864	25.838447088290543	27.91783507119327	23.425117747951322
27	24.23640669147046	25.341741866666084	27.156016154228563	23.265835287634893
28	23.13646612590666	25.23387589018764	27.62520616089616	24.004451823009543
29	22.745189216090377	25.469698363944154	28.00635539116529	23.77875702880018
30	23.50879447398956	25.51804986629829	27.865439075745783	23.107716583966358
31	23.24335126005213	25.725993996355122	26.756131465992507	24.27452327760024
32	22.943060116490095	26.047193897691923	26.70750771418817	24.30223827162981
33	22.652842202585173	25.45020530331039	27.384537646926223	24.512414847178217
34	23.23322358050498	25.99432305446459	27.830209996890908	22.94224336813952
35	24.45584141142827	25.790135966820415	27.28892364001871	22.465098981732606
36	23.04395576273134	26.755314717641927	26.66106195798538	23.53966756164136
37	24.174825528939845	26.419631145554902	26.714259500552938	22.691283824952315
38	23.285617053714848	26.15322207399744	26.74449375187172	23.816667120415996
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	328.0
1	324.0
2	320.0
3	320.0
4	1495.0
5	2670.0
6	2670.0
7	3395.0
8	4120.0
9	4232.5
10	4345.0
11	4345.0
12	5497.0
13	6649.0
14	8144.5
15	9640.0
16	9640.0
17	14410.0
18	19180.0
19	19180.0
20	21429.0
21	23678.0
22	22843.5
23	22009.0
24	22009.0
25	24533.5
26	27058.0
27	27058.0
28	34725.5
29	42393.0
30	50092.0
31	57791.0
32	57791.0
33	66157.5
34	74524.0
35	74524.0
36	79503.5
37	84483.0
38	95070.5
39	105658.0
40	105658.0
41	109458.5
42	113259.0
43	125989.5
44	138720.0
45	138720.0
46	156808.5
47	174897.0
48	174897.0
49	172165.0
50	169433.0
51	160110.0
52	150787.0
53	150787.0
54	141562.0
55	132337.0
56	132337.0
57	128198.0
58	124059.0
59	111478.0
60	98897.0
61	98897.0
62	93864.0
63	88831.0
64	74066.5
65	59302.0
66	59302.0
67	49838.5
68	40375.0
69	40375.0
70	32550.0
71	24725.0
72	19209.5
73	13694.0
74	13694.0
75	10386.0
76	7078.0
77	7078.0
78	6913.5
79	6749.0
80	5249.5
81	3750.0
82	3750.0
83	3434.0
84	3118.0
85	3118.0
86	2002.5
87	887.0
88	712.0
89	537.0
90	537.0
91	332.0
92	127.0
93	105.0
94	83.0
95	83.0
96	59.5
97	36.0
98	36.0
99	30.0
100	24.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010182129437189602
2	0.0
3	0.0
4	3.266993402306824E-4
5	5.4449890038447064E-5
6	2.1779956015378826E-4
7	2.1779956015378826E-4
8	0.00223244549157633
9	0.003920392082768189
10	0.0010889978007689414
11	0.005553888783921601
12	3.8114923026912945E-4
13	5.4449890038447064E-5
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.0889978007689413E-4
22	5.4449890038447064E-5
23	2.1779956015378826E-4
24	0.0
25	1.0889978007689413E-4
26	5.4449890038447064E-5
27	2.1779956015378826E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	5.4449890038447064E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1836551.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.997400729071174
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.33516782659281	28.015642592751565
2	16.63929732831506	13.643358808381917
3	6.424427009146734	7.9015442564597045
4	3.1523481325102334	5.169523185042448
5	1.6671708435774808	3.417483557898481
6	0.9830730399056747	2.418206361777547
7	0.6092689266365984	1.7484909635964183
8	0.43035756712440704	1.4114833308949968
9	0.27872224518785244	1.0284198820265533
>10	1.2331768772803393	8.731894919902837
>50	0.10275770942297696	2.9836273321496876
>100	0.11874870802978978	10.571032040270405
>500	0.01614417873655542	4.5739549124510335
>1k	0.008672492709719853	5.736219397553218
>5k	4.0026889429476244E-4	1.2871444653115354
>10k+	2.6684592952984164E-4	1.361973993531593
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	14494	0.7891967062172519	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	10405	0.5665511058500416	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	8754	0.4766543373965656	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	8500	0.4628240653268001	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	6277	0.34178195977133224	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4519	0.2460590530837423	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	3683	0.20053894501160055	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	3543	0.19291596040621795	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2451	0.13345668048423376	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2216	0.12066095632519869	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	2163	0.117775112153161	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	2125	0.11570601633170002	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2110	0.1148892679811233	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	2090	0.11380027018035437	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2029	0.1104788268880091	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1929	0.10503383788416439	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1917	0.10438043920370302	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1853	0.10089564624124242	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	1841	0.10024224756078105	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	5.444989003844707E-5	0.0
7	0.0	0.0	0.0	5.444989003844707E-5	0.0
8	0.0	0.0	0.0	5.444989003844707E-5	0.0
9	0.0	0.0	5.444989003844707E-5	1.0889978007689414E-4	0.0
10	0.0	0.0	5.444989003844707E-5	1.0889978007689414E-4	0.0
11	0.0	0.0	5.444989003844707E-5	1.0889978007689414E-4	0.0
12	0.0	0.0	5.444989003844707E-5	1.0889978007689414E-4	0.0
13	0.0	0.0	5.444989003844707E-5	1.633496701153412E-4	0.0
14	0.0	0.0	5.444989003844707E-5	3.266993402306824E-4	0.0
15	0.0	0.0	5.444989003844707E-5	3.266993402306824E-4	0.0
16	0.0	0.0	5.444989003844707E-5	5.989487904229177E-4	0.0
17	0.0	0.0	5.444989003844707E-5	8.16748350576706E-4	0.0
18	0.0	0.0	5.444989003844707E-5	9.800980206920472E-4	0.0
19	0.0	0.0	5.444989003844707E-5	9.800980206920472E-4	0.0
20	0.0	0.0	5.444989003844707E-5	0.0013067973609227296	0.0
21	0.0	0.0	5.444989003844707E-5	0.0020690958214609886	0.0
22	0.0	0.0	5.444989003844707E-5	0.003103643732191483	0.0
23	0.0	0.0	5.444989003844707E-5	0.00500938988353713	0.0
24	0.0	0.0	5.444989003844707E-5	0.007187385485075013	0.0
25	0.0	0.0	5.444989003844707E-5	0.008385283065920849	0.0
26	0.0	0.0	5.444989003844707E-5	0.010781078227612519	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGCC	40	1.5933772E-4	24.00151	4
GTATTAC	120	8.185452E-11	18.668348	1
CTAGCGG	525	0.0	18.589622	29
TAGCGGC	540	0.0	18.369528	30
TCTAGCG	560	0.0	17.42777	28
ACACCGT	75	3.2321346E-5	17.06867	6
GATATAC	790	0.0	17.014193	1
AGCGGCG	585	0.0	16.956486	31
CGGTCCA	610	0.0	16.787941	10
CATCGTT	420	0.0	16.761135	28
TAGGACG	500	0.0	16.641048	4
TACCGTC	430	0.0	16.374014	7
ATCGTTT	430	0.0	16.37134	29
ATACCGT	450	0.0	16.357475	6
GCGCAAG	600	0.0	16.268135	1
ACCGTCG	425	0.0	16.190136	8
CCGGTCC	640	0.0	16.001007	9
TCTTGCG	630	0.0	16.000137	2
CGTTTAT	425	0.0	15.811038	31
CAAGACG	610	0.0	15.738695	4
>>END_MODULE