FastQCFastQC Report
Thu 2 Feb 2017
SRR4062872_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062872_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1028492
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT36680.3566386515403134No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT25210.24511615063607692No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT13380.13009337943318955No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC12910.12552358209883985No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT12090.11755074419635739No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11650.1132726360535619No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC10820.10520256842056136No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG10800.1050081089595252No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA10790.10491087922900712No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC10750.10452196030693482No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA10640.10345243327123593No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG10370.10082723054724782No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTAG350.00205662622.8757761
GTATTAG1650.022.321211
CGCTTCG2700.019.55531732
AGATACG507.1934663E-419.20069917
ATAGGTC507.198176E-419.1988333
GACGTGA1003.263267E-919.1988337
GGCGTTA1251.4733814E-1017.9197831
ATACGAG450.008850276517.77756119
ATTATAC1101.0635631E-817.4534853
ACGCTTC2950.017.3557231
AAGACGG3050.016.7817845
TAGGACG2050.016.386064
AACGCTT2950.016.27098830
GTAAACG2950.016.27098827
CAAGACG3150.016.2498194
ATAACGA1304.794856E-916.00058412
TATACAG1900.015.9951385
AAACGCT3150.015.7458429
TACCGTC1950.015.5887957
CGGCGTT1753.6379788E-1215.54266730
Produced by FastQC (version 0.11.5)