##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062872_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1028492
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.227958020091553	32.0	32.0	32.0	32.0	32.0
2	30.8206782357082	32.0	32.0	32.0	32.0	32.0
3	30.877529431439427	32.0	32.0	32.0	32.0	32.0
4	30.937953819767195	32.0	32.0	32.0	32.0	32.0
5	30.841958906826694	32.0	32.0	32.0	32.0	32.0
6	34.520900502872166	36.0	36.0	36.0	32.0	36.0
7	34.43906807247893	36.0	36.0	36.0	32.0	36.0
8	34.40901047358657	36.0	36.0	36.0	32.0	36.0
9	34.57576723980352	36.0	36.0	36.0	32.0	36.0
10	34.256190616942085	36.0	36.0	36.0	32.0	36.0
11	34.575993785075624	36.0	36.0	36.0	32.0	36.0
12	34.37403888411383	36.0	36.0	36.0	32.0	36.0
13	34.47524433831279	36.0	36.0	36.0	32.0	36.0
14	34.367439902303566	36.0	36.0	36.0	32.0	36.0
15	34.304396145035646	36.0	36.0	36.0	32.0	36.0
16	34.32251879450691	36.0	36.0	36.0	32.0	36.0
17	34.24180547831194	36.0	36.0	36.0	32.0	36.0
18	34.27565892588372	36.0	36.0	36.0	32.0	36.0
19	34.276271473185986	36.0	36.0	36.0	32.0	36.0
20	34.248799212828104	36.0	36.0	36.0	32.0	36.0
21	34.22685251805556	36.0	36.0	36.0	32.0	36.0
22	34.194852269147454	36.0	36.0	36.0	32.0	36.0
23	34.1422937660186	36.0	36.0	36.0	32.0	36.0
24	34.124732132092426	36.0	36.0	36.0	32.0	36.0
25	34.0991859926961	36.0	36.0	36.0	32.0	36.0
26	34.0684263951494	36.0	36.0	36.0	32.0	36.0
27	34.05327605854008	36.0	36.0	36.0	32.0	36.0
28	34.040805373303826	36.0	36.0	36.0	32.0	36.0
29	34.01276334672511	36.0	36.0	36.0	32.0	36.0
30	33.97469693492997	36.0	36.0	36.0	32.0	36.0
31	33.98406015797887	36.0	36.0	36.0	32.0	36.0
32	33.944102627925155	36.0	36.0	36.0	32.0	36.0
33	33.904885988417995	36.0	36.0	36.0	32.0	36.0
34	33.89085671060154	36.0	36.0	36.0	32.0	36.0
35	33.85422249273694	36.0	36.0	36.0	32.0	36.0
36	33.80045931324697	36.0	36.0	36.0	32.0	36.0
37	33.80061779770771	36.0	36.0	36.0	32.0	36.0
38	33.185762261641315	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	1.0
8	4.0
9	6.0
10	13.0
11	9.0
12	4.0
13	4.0
14	125.0
15	288.0
16	396.0
17	413.0
18	575.0
19	725.0
20	975.0
21	1391.0
22	2136.0
23	3125.0
24	4722.0
25	7031.0
26	10189.0
27	14567.0
28	20415.0
29	27361.0
30	36696.0
31	49092.0
32	67264.0
33	100697.0
34	222620.0
35	457646.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.20751284215322	17.040712327006922	11.09735310981124	25.65442172102862
2	16.7298830391721	19.69685325467848	37.66492760841713	25.90833609773229
3	19.201526273669348	24.06224504977212	28.351778075763306	28.384450600795226
4	12.432520729942906	14.99245476749794	36.22927005709485	36.34575444546431
5	14.371846104483271	36.71742068469309	33.772326420286056	15.138406790537584
6	34.1253042084067	35.5540020359965	16.932218040251282	13.388475715345521
7	30.074905784391127	30.58380619392275	20.953979223951183	18.387308797734935
8	28.334798266970544	32.78725663303801	19.605284650866125	19.27266044912532
9	27.72076317782689	13.982551101664676	18.62752056657874	39.6691651539297
10	15.739543367629699	26.747665986702785	31.74695620552929	25.765834440138224
11	37.72093584036808	21.253011767039453	22.035730884637896	18.990321507954565
12	24.79865507521447	23.85170434601671	28.935232226078046	22.414408352690774
13	29.698913982091597	19.46692935437947	25.414549994943325	25.419606668585608
14	23.51535789715246	19.74570107234956	25.491444929774133	31.247496100723847
15	25.19637638523518	27.384744056678546	22.7054573038789	24.71342225420737
16	25.475604253697092	25.874874559499638	24.341874956241686	24.307646230561584
17	23.63665008227363	26.05253842353609	25.675305073735068	24.635506420455208
18	24.731069876617397	24.76928357579099	26.934045877830904	23.565600669760713
19	25.1984613170597	25.058536850989682	26.046953944528067	23.69604788742255
20	25.529546064478993	24.133823660966055	25.89340235777322	24.44322791678173
21	26.687922491112616	24.285508700691544	24.848506063646898	24.178062744548942
22	25.42113635833249	24.647124487557466	25.454488879899806	24.477250274210235
23	23.927455960544382	24.406162606622352	26.062236725930475	25.604144706902787
24	24.299553456819897	25.362621846386034	25.95434990625705	24.38347479053702
25	24.582152995165842	24.770520865850372	25.912592251146787	24.734733887837
26	24.2273831045881	25.74757534588944	26.128304147366844	23.896737402155615
27	24.986143389181148	25.088244732056904	25.470492711908904	24.45511916685304
28	24.073249373804003	25.100055229708918	26.11208130435459	24.71461409213249
29	23.96165978848121	25.34997063319499	26.155515708323446	24.532853870000352
30	24.025770719934968	25.59336635376983	26.361995772295117	24.01886715400008
31	24.552863868755797	25.35583226536657	25.24663712012633	24.844666745751308
32	24.164543668929035	25.442231773011027	25.2913206666537	25.101903891406234
33	23.888101797065147	25.036683232869734	25.895591108120485	25.179623861944634
34	24.61581846735806	25.370616178265287	25.997637002786	24.01592835159065
35	25.26810511821131	25.126516053243712	25.888140748866125	23.717238079678857
36	24.062310979203094	25.913085928003376	25.538107565110675	24.486495527682855
37	24.95298476259007	25.758321259444372	25.24402220947306	24.0446717684925
38	24.131929251383177	25.55672238266095	25.753235514327667	24.55811285162821
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	71.0
1	125.5
2	180.0
3	180.0
4	551.0
5	922.0
6	922.0
7	1152.0
8	1382.0
9	1383.0
10	1384.0
11	1384.0
12	1735.5
13	2087.0
14	2625.0
15	3163.0
16	3163.0
17	4605.0
18	6047.0
19	6047.0
20	7113.5
21	8180.0
22	8467.0
23	8754.0
24	8754.0
25	10135.0
26	11516.0
27	11516.0
28	14903.0
29	18290.0
30	22242.5
31	26195.0
32	26195.0
33	32306.5
34	38418.0
35	38418.0
36	42525.5
37	46633.0
38	52537.0
39	58441.0
40	58441.0
41	61966.5
42	65492.0
43	72688.5
44	79885.0
45	79885.0
46	83657.5
47	87430.0
48	87430.0
49	90585.0
50	93740.0
51	93372.0
52	93004.0
53	93004.0
54	88014.0
55	83024.0
56	83024.0
57	80235.5
58	77447.0
59	69007.5
60	60568.0
61	60568.0
62	57289.0
63	54010.0
64	45310.5
65	36611.0
66	36611.0
67	30610.0
68	24609.0
69	24609.0
70	19994.0
71	15379.0
72	12313.5
73	9248.0
74	9248.0
75	7128.5
76	5009.0
77	5009.0
78	4618.0
79	4227.0
80	3303.5
81	2380.0
82	2380.0
83	2307.5
84	2235.0
85	2235.0
86	1519.5
87	804.0
88	682.5
89	561.0
90	561.0
91	412.5
92	264.0
93	242.5
94	221.0
95	221.0
96	191.0
97	161.0
98	161.0
99	340.5
100	520.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11774520365739354
2	0.05260128421028068
3	0.010209121704398284
4	0.002722432454506209
5	1.9445946103615778E-4
6	2.916891915542367E-4
7	0.0
8	3.8891892207231556E-4
9	2.916891915542367E-4
10	5.833783831084734E-4
11	0.0025279729934700513
12	0.0032085811070966036
13	0.014390000116675676
14	0.009334054129735574
15	0.0230434461327847
16	0.011278648740097152
17	0.021001621791905043
18	0.006319932483675128
19	0.0077783784414463125
20	0.006222702753157049
21	0.006611621675229364
22	0.008167297363518627
23	0.009334054129735574
24	0.014390000116675676
25	0.017987500145844597
26	0.01954317583413386
27	0.009917432512844047
28	0.0058337838310847335
29	0.011667567662169467
30	0.003694729759686998
31	0.005639324370048576
32	0.007000540597301681
33	0.009042364938181337
34	0.013320473080976808
35	0.015945675804964938
36	0.016140135266001096
37	0.009917432512844047
38	0.006028243292120892
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1028492.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.82149553899008
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.51647834658272	47.360996718787064
2	12.417054775606793	14.607794641815008
3	3.4659968378498913	6.1162535260722315
4	1.2937008307703355	3.0438967058378017
5	0.6242733757894646	1.8360346794555134
6	0.3759167857507278	1.3267192521640752
7	0.244629493102584	1.0072630846067334
8	0.16138567574796706	0.7594357444852754
9	0.12170563236621831	0.6443016580169529
>10	0.6273166148671342	6.991814680687858
>50	0.06830149671196754	2.8348734250985506
>100	0.07493273675796634	9.136747281388354
>500	0.005815178667428286	2.315240662341629
>1k	0.002492219428897837	2.0186279392429864
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3668	0.3566386515403134	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2521	0.24511615063607692	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1338	0.13009337943318955	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1291	0.12552358209883985	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1209	0.11755074419635739	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1165	0.1132726360535619	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1082	0.10520256842056136	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1080	0.1050081089595252	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1079	0.10491087922900712	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1075	0.10452196030693482	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1064	0.10345243327123593	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1037	0.10082723054724782	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.722973051807889E-5
2	0.0	0.0	0.0	9.722973051807889E-5	9.722973051807889E-5
3	0.0	0.0	0.0	9.722973051807889E-5	9.722973051807889E-5
4	0.0	0.0	0.0	9.722973051807889E-5	9.722973051807889E-5
5	0.0	0.0	0.0	1.9445946103615778E-4	9.722973051807889E-5
6	0.0	0.0	0.0	1.9445946103615778E-4	9.722973051807889E-5
7	0.0	0.0	0.0	1.9445946103615778E-4	9.722973051807889E-5
8	0.0	0.0	0.0	1.9445946103615778E-4	9.722973051807889E-5
9	0.0	0.0	0.0	1.9445946103615778E-4	9.722973051807889E-5
10	0.0	0.0	0.0	1.9445946103615778E-4	9.722973051807889E-5
11	0.0	0.0	0.0	1.9445946103615778E-4	9.722973051807889E-5
12	0.0	0.0	0.0	3.8891892207231556E-4	1.9445946103615778E-4
13	0.0	0.0	0.0	3.8891892207231556E-4	3.8891892207231556E-4
14	0.0	0.0	0.0	4.861486525903945E-4	3.8891892207231556E-4
15	0.0	0.0	0.0	5.833783831084734E-4	3.8891892207231556E-4
16	0.0	0.0	0.0	7.778378441446311E-4	4.861486525903945E-4
17	0.0	0.0	0.0	0.001069527035698868	4.861486525903945E-4
18	0.0	0.0	0.0	0.0012639864967350257	4.861486525903945E-4
19	0.0	0.0	0.0	0.0012639864967350257	4.861486525903945E-4
20	0.0	0.0	0.0	0.0016529054188073413	6.806081136265523E-4
21	0.0	0.0	0.0	0.0020418243408796568	7.778378441446311E-4
22	0.0	0.0	0.0	0.0032085811070966036	9.72297305180789E-4
23	0.0	0.0	0.0	0.004180878412277393	9.72297305180789E-4
24	0.0	0.0	0.0	0.005347635178494339	9.72297305180789E-4
25	0.0	0.0	0.0	0.0067088514057474435	9.72297305180789E-4
26	0.0	0.0	0.0	0.008070067633000548	9.72297305180789E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCTAG	35	0.002056626	22.875776	1
GTATTAG	165	0.0	22.32121	1
CGCTTCG	270	0.0	19.555317	32
AGATACG	50	7.1934663E-4	19.200699	17
ATAGGTC	50	7.198176E-4	19.198833	3
GACGTGA	100	3.263267E-9	19.198833	7
GGCGTTA	125	1.4733814E-10	17.91978	31
ATACGAG	45	0.0088502765	17.777561	19
ATTATAC	110	1.0635631E-8	17.453485	3
ACGCTTC	295	0.0	17.35572	31
AAGACGG	305	0.0	16.781784	5
TAGGACG	205	0.0	16.38606	4
AACGCTT	295	0.0	16.270988	30
GTAAACG	295	0.0	16.270988	27
CAAGACG	315	0.0	16.249819	4
ATAACGA	130	4.794856E-9	16.000584	12
TATACAG	190	0.0	15.995138	5
AAACGCT	315	0.0	15.74584	29
TACCGTC	195	0.0	15.588795	7
CGGCGTT	175	3.6379788E-12	15.542667	30
>>END_MODULE