FastQCFastQC Report
Thu 2 Feb 2017
SRR4062872_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062872_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1028492
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT41730.40573966545194323No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT31350.3048152051741773No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT18360.17851378523119285No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14480.14078864979017824No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC13810.13427425784546695No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT12750.12396790641055058No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC11680.11356432524511616No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA11490.11171696036527265No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG11390.11074466306009187No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC11370.11055020359905571No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA10670.10374412246279019No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG10640.10345243327123593No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACCGT453.5356582E-421.3340136
CGTCGTA2450.020.24554410
CTAGCGG2700.020.1478129
ACCGTCG2500.019.2006138
TCTAGCG2900.018.75830728
CCGTCGT2500.018.5605939
GCGCAAG3150.018.2889651
TAGCGGC2900.018.20659330
ATACCGT2850.017.9654856
GTCGTAG2650.017.50914211
TACCGTC2800.017.1434047
CGATAAC854.98139E-616.94171710
GTATAGA1051.1862903E-716.7648871
AGAACCG2100.016.762445
TAGACTG1151.839362E-816.6961845
CAAGACG3600.016.0005114
GATAACG908.580406E-615.99973211
TAACGAA908.580406E-615.99973213
CGCAAGA3600.015.9997322
CGGACCA3550.015.7751519
Produced by FastQC (version 0.11.5)