FastQCFastQC Report
Thu 2 Feb 2017
SRR4062871_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062871_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences641558
Sequences flagged as poor quality0
Sequence length38
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT117311.8285174528257773No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT88401.3778956851913624No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT50200.7824701741697556No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA46210.720277823672996No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT39440.6147534595469154No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA36990.5765651741541684No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC29390.45810355416034093No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA27060.42178571539907533No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA22820.3556966010867295No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA22460.350085261192285No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA19890.3100265291680565No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA19300.30083016656327255No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG19150.2984921082739207No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18170.2832167941168218No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC16690.2601479523285502No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16020.24970462530277854No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA15370.23957303938225383No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15250.23770259275077235No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG13970.2177511620149698No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAAT13640.2126074337783957No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT13530.21089285769953767No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG13470.20995763438379694No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12750.198734954594908No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG12440.19390296746358085No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAA10790.1681843262807104No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA10070.15696164649182145No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA9760.15212965936049427No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA9730.15166204770262393No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAAT9490.14792115443966095No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA9460.14745354278179057No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA9260.14433613172932142No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA9090.14168633233472266No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA8900.13872479183487696No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC8740.13623086299290166No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA8420.13124300530895103No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT7980.12438470099351889No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG7540.11752639667808677No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA6990.10895351628379664No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA6760.10536849357345712No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA6640.10349804694197563No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG6520.10162760031049414No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTAT308.388633E-426.6797981
CTCTTAC350.002059647322.8683991
GTCGATG350.002064322122.85948215
ACCAATC350.002067131222.85413727
ATAATAC350.002069005522.8505743
TAGGACC7100.022.3034484
GACCTAG957.2759576E-1121.8952717
ATAGGAC1300.020.9170653
GGACCTA1001.4551915E-1020.7940226
GAGCTTA707.759845E-720.5751389
GCAGTTC400.004476683720.00360513
ATAGCGA400.00448274819.99892817
GAATACG400.00448274819.99892823
TAGACCC400.00448881919.9942514
GAACGTA400.00448881919.9942516
AGGACCT11800.019.3842585
GTCCTAT1003.2341632E-919.2094541
AGCGAGA507.1937905E-419.1989719
AGTACTG507.2051195E-419.194485
AAGCGTT701.7824863E-518.28901311
Produced by FastQC (version 0.11.5)