##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062871_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	641558
Sequences flagged as poor quality	0
Sequence length	38
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.71406170603437	32.0	32.0	32.0	32.0	32.0
2	30.558303380208805	32.0	32.0	32.0	32.0	32.0
3	30.706296235102673	32.0	32.0	32.0	32.0	32.0
4	30.637014891872596	32.0	32.0	32.0	32.0	32.0
5	30.763226395742862	32.0	32.0	32.0	32.0	32.0
6	34.293047549870785	36.0	36.0	36.0	32.0	36.0
7	34.024039915331116	36.0	36.0	36.0	32.0	36.0
8	33.95168948091989	36.0	36.0	36.0	32.0	36.0
9	33.92020986411205	36.0	36.0	36.0	32.0	36.0
10	33.88794310101347	36.0	36.0	36.0	32.0	36.0
11	34.22307881750364	36.0	36.0	36.0	32.0	36.0
12	34.060568179338425	36.0	36.0	36.0	32.0	36.0
13	34.11822781416489	36.0	36.0	36.0	32.0	36.0
14	34.06613587547813	36.0	36.0	36.0	32.0	36.0
15	34.10040089906135	36.0	36.0	36.0	32.0	36.0
16	34.07667584224653	36.0	36.0	36.0	32.0	36.0
17	34.01420136604953	36.0	36.0	36.0	32.0	36.0
18	34.08102463066473	36.0	36.0	36.0	32.0	36.0
19	33.98716405999146	36.0	36.0	36.0	32.0	36.0
20	33.82319447345369	36.0	36.0	36.0	32.0	36.0
21	33.71937845058436	36.0	36.0	36.0	27.0	36.0
22	33.752115942751864	36.0	36.0	36.0	27.0	36.0
23	33.81288051898659	36.0	36.0	36.0	32.0	36.0
24	33.81208246175716	36.0	36.0	36.0	32.0	36.0
25	33.798842817017324	36.0	36.0	36.0	32.0	36.0
26	33.80641033234719	36.0	36.0	36.0	27.0	36.0
27	33.78837455070313	36.0	36.0	36.0	27.0	36.0
28	33.703998703157005	36.0	36.0	36.0	27.0	36.0
29	33.64689240879235	36.0	36.0	36.0	27.0	36.0
30	33.533342269911685	36.0	36.0	36.0	21.0	36.0
31	33.54614391839865	36.0	36.0	36.0	21.0	36.0
32	33.444962107868655	36.0	36.0	36.0	21.0	36.0
33	33.383611458356064	36.0	36.0	36.0	21.0	36.0
34	33.2513100919948	36.0	36.0	36.0	21.0	36.0
35	33.17473556560748	36.0	36.0	36.0	21.0	36.0
36	33.08913613422325	36.0	36.0	36.0	14.0	36.0
37	33.007639215784074	36.0	36.0	36.0	14.0	36.0
38	32.30700731656374	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	0.0
9	8.0
10	6.0
11	7.0
12	2.0
13	5.0
14	389.0
15	736.0
16	778.0
17	856.0
18	1098.0
19	1343.0
20	1582.0
21	2023.0
22	2591.0
23	3330.0
24	4474.0
25	5945.0
26	7959.0
27	11141.0
28	14850.0
29	20003.0
30	26334.0
31	34287.0
32	44003.0
33	63238.0
34	132416.0
35	262149.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.59553311152864	19.57150704427563	14.189206059887265	20.643753784308462
2	10.560330374868803	23.100196347224237	48.36832181606367	17.97115146184329
3	18.885781452658883	31.91674773022049	30.417539658784793	18.779931158335827
4	9.39867848619799	18.967573187516855	38.907966337093576	32.72578198919158
5	8.809180152067311	43.515161528653685	34.540602720252885	13.13505559902612
6	25.1747706743771	40.247055981170746	21.31820342760948	13.259969916842671
7	23.25152207594637	35.22300399963838	22.948353851093746	18.577120073321506
8	24.701349228121806	39.53490909544355	20.903527695338802	14.860213981095843
9	28.606722448063447	14.62406788537796	21.479007157642716	35.29020250891588
10	15.684358194996491	31.410646091497156	33.91551710700647	18.989478606499883
11	31.54528648341953	23.322079128594098	27.50842506601324	17.624209321973133
12	25.20349661904124	27.098018187656457	34.31088609489131	13.387599098410996
13	31.796873660268172	24.468199540730186	27.463898368235867	16.271028430765778
14	20.760611332472827	25.697757075780537	30.592033472385904	22.949598119360733
15	20.426853510573114	36.115723482244064	28.497593511602126	14.959829495580697
16	16.064694649051013	29.497486262130245	36.19314860282942	18.244670485989325
17	15.925620466064766	31.04598753065498	33.02458790202257	20.003804101257693
18	16.259631587397838	27.792751153919898	40.75157168488673	15.196045573795535
19	21.38332034294622	26.802805923616525	32.86360093530787	18.950272798129383
20	22.245951292359635	27.695669998955573	35.505166788516306	14.55321192016848
21	22.10286747570167	26.045627080069522	30.337253801607154	21.514251642621648
22	20.993616474017724	31.26509169985737	31.567354383120676	16.17393744300423
23	18.21656726143524	31.633779279855084	33.29945345309767	16.850200005612002
24	20.05765009603153	30.514485794816792	34.34997879823401	15.077885310917663
25	19.623469839547518	30.205039941630968	34.4838768014268	15.687613417394722
26	14.855205207155942	31.963674630705068	36.91592937599875	16.265190786140234
27	16.37133445885381	32.23524936825876	35.36581753785381	16.02759863503362
28	15.406702592153238	32.97423434910906	32.938381607231655	18.680681451506047
29	16.655468656223157	30.307740033640794	33.10438468361593	19.93240662652011
30	16.145545123945297	31.636876045347627	33.977831226141134	18.239747604565938
31	18.546623373181838	30.009368477334053	33.38482618434856	18.059181965135547
32	15.738541062621197	33.99942011635085	33.75624310219047	16.50579571883749
33	15.396330770058162	33.464380971384124	35.24116246841373	15.898125790143991
34	15.907971290135507	33.66939381278724	35.45269170569271	14.969943191384546
35	15.628649067475692	34.71737412130065	34.22488155390057	15.42909525732308
36	14.889858913399328	34.65476654454751	34.415464962195024	16.039909579858133
37	15.59398763480587	34.68318477017139	31.35181565073159	18.37101194429115
38	16.673239730821333	37.246047198237896	30.23240588177526	15.848307189165512
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2134.0
1	1620.0
2	1106.0
3	1106.0
4	2532.5
5	3959.0
6	3959.0
7	4597.0
8	5235.0
9	4900.5
10	4566.0
11	4566.0
12	5674.0
13	6782.0
14	7746.0
15	8710.0
16	8710.0
17	12452.0
18	16194.0
19	16194.0
20	17600.0
21	19006.0
22	17702.5
23	16399.0
24	16399.0
25	19271.0
26	22143.0
27	22143.0
28	29937.5
29	37732.0
30	47937.5
31	58143.0
32	58143.0
33	62716.5
34	67290.0
35	67290.0
36	66988.0
37	66686.0
38	66743.0
39	66800.0
40	66800.0
41	60084.0
42	53368.0
43	47753.5
44	42139.0
45	42139.0
46	45536.5
47	48934.0
48	48934.0
49	41358.5
50	33783.0
51	25948.0
52	18113.0
53	18113.0
54	15038.0
55	11963.0
56	11963.0
57	10293.0
58	8623.0
59	7595.5
60	6568.0
61	6568.0
62	5769.5
63	4971.0
64	4224.0
65	3477.0
66	3477.0
67	2931.0
68	2385.0
69	2385.0
70	2011.0
71	1637.0
72	1345.5
73	1054.0
74	1054.0
75	846.0
76	638.0
77	638.0
78	493.0
79	348.0
80	254.5
81	161.0
82	161.0
83	126.0
84	91.0
85	91.0
86	72.5
87	54.0
88	41.0
89	28.0
90	28.0
91	29.5
92	31.0
93	26.0
94	21.0
95	21.0
96	28.0
97	35.0
98	35.0
99	143.0
100	251.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11752639667808677
2	0.05408708176033967
3	0.013404867525617325
4	0.003273281605092603
5	0.0
6	4.6761165787037183E-4
7	0.0
8	9.352233157407437E-4
9	9.352233157407437E-4
10	0.0012469644209876583
11	0.0031174110524691454
12	0.0037408932629629747
13	0.016366408025463013
14	0.011534420894135838
15	0.026030382288117365
16	0.012937255867746954
17	0.022601230130401304
18	0.0076376570785494065
19	0.009040492052160521
20	0.008884621499537065
21	0.008261139289043236
22	0.009819844815277808
23	0.011846161999382753
24	0.01589879636759264
25	0.019016207420061784
26	0.020730783498919818
27	0.012002032552006209
28	0.007014174868055578
29	0.011690291446759296
30	0.0038967638155864314
31	0.007014174868055578
32	0.007793527631172863
33	0.009508103710030892
34	0.016522278578086472
35	0.019795560183179073
36	0.016834019683333385
37	0.012469644209876582
38	0.00794939818379632
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	641558.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.30791010373156
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.6387773559507	30.83171905272046
2	13.396757363124676	11.067840976749158
3	4.8758649498078235	6.042353730737916
4	2.224624844845257	3.675784124215826
5	1.1943371700170724	2.466778622630519
6	0.7370542846077824	1.8267703278089071
7	0.5031445429090763	1.454869468336986
8	0.37565095899454903	1.2413884835621904
9	0.2445128485789422	0.9090283291475644
>10	1.5125569703528856	11.714603028530336
>50	0.15626683401697344	4.43687907720313
>100	0.11898872135917749	9.725203387299224
>500	0.011672944183969943	3.395515161293936
>1k	0.00866057149133254	7.230751869128318
>5k	7.530931731593512E-4	2.1558332631944093
>10k+	3.765465865796756E-4	1.8246810974410979
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	11731	1.8285174528257773	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	8840	1.3778956851913624	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	5020	0.7824701741697556	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	4621	0.720277823672996	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3944	0.6147534595469154	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	3699	0.5765651741541684	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	2939	0.45810355416034093	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	2706	0.42178571539907533	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA	2282	0.3556966010867295	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	2246	0.350085261192285	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	1989	0.3100265291680565	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	1930	0.30083016656327255	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG	1915	0.2984921082739207	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1817	0.2832167941168218	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	1669	0.2601479523285502	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1602	0.24970462530277854	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA	1537	0.23957303938225383	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1525	0.23770259275077235	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG	1397	0.2177511620149698	No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAAT	1364	0.2126074337783957	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	1353	0.21089285769953767	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	1347	0.20995763438379694	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1275	0.198734954594908	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	1244	0.19390296746358085	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAA	1079	0.1681843262807104	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	1007	0.15696164649182145	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	976	0.15212965936049427	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	973	0.15166204770262393	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAAT	949	0.14792115443966095	No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA	946	0.14745354278179057	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA	926	0.14433613172932142	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA	909	0.14168633233472266	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA	890	0.13872479183487696	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	874	0.13623086299290166	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA	842	0.13124300530895103	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT	798	0.12438470099351889	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	754	0.11752639667808677	No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA	699	0.10895351628379664	No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA	676	0.10536849357345712	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA	664	0.10349804694197563	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	652	0.10162760031049414	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	1.5587055262345726E-4	0.0
12	0.0	0.0	0.0	1.5587055262345726E-4	0.0
13	0.0	0.0	0.0	1.5587055262345726E-4	0.0
14	0.0	0.0	0.0	1.5587055262345726E-4	0.0
15	0.0	0.0	0.0	1.5587055262345726E-4	0.0
16	0.0	0.0	0.0	4.6761165787037183E-4	0.0
17	0.0	0.0	0.0	7.793527631172864E-4	0.0
18	0.0	0.0	0.0	7.793527631172864E-4	0.0
19	0.0	0.0	0.0	7.793527631172864E-4	0.0
20	0.0	0.0	0.0	0.0010910938683642009	0.0
21	0.0	0.0	0.0	0.002338058289351859	0.0
22	0.0	0.0	0.0	0.004208504920833346	0.0
23	0.0	0.0	0.0	0.00919636260478398	0.0
24	0.0	0.0	0.0	0.016366408025463013	0.0
25	0.0	0.0	0.0	0.02010730128842599	0.0
26	0.0	0.0	0.0	0.02462754731450625	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCCTAT	30	8.388633E-4	26.679798	1
CTCTTAC	35	0.0020596473	22.868399	1
GTCGATG	35	0.0020643221	22.859482	15
ACCAATC	35	0.0020671312	22.854137	27
ATAATAC	35	0.0020690055	22.850574	3
TAGGACC	710	0.0	22.303448	4
GACCTAG	95	7.2759576E-11	21.895271	7
ATAGGAC	130	0.0	20.917065	3
GGACCTA	100	1.4551915E-10	20.794022	6
GAGCTTA	70	7.759845E-7	20.575138	9
GCAGTTC	40	0.0044766837	20.003605	13
ATAGCGA	40	0.004482748	19.998928	17
GAATACG	40	0.004482748	19.998928	23
TAGACCC	40	0.004488819	19.994251	4
GAACGTA	40	0.004488819	19.994251	6
AGGACCT	1180	0.0	19.384258	5
GTCCTAT	100	3.2341632E-9	19.209454	1
AGCGAGA	50	7.1937905E-4	19.19897	19
AGTACTG	50	7.2051195E-4	19.19448	5
AAGCGTT	70	1.7824863E-5	18.289013	11
>>END_MODULE