FastQCFastQC Report
Thu 2 Feb 2017
SRR4062871_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062871_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences641558
Sequences flagged as poor quality0
Sequence length38
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT148782.3190420819317974No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT118951.854080223456024No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT74001.1534420894135837No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT49840.7768588342753111No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA37670.5871643717325635No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA36070.5622250833128104No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC35530.5538080734711437No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22110.34462979185046405No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21280.3316925359827171No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA20870.3253018433251553No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20710.32280791448318No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG20200.3148585162993837No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA19290.30067429601064904No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA19270.30036255490540215No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA17560.27370869040679097No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA17220.2684090916175934No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA17010.2651358100125008No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT16800.2618625284074082No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC15570.24269045043472298No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG14030.21868638533071053No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG13710.21369852764675992No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAAT12970.20216410675262408No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG12670.19748799017392038No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAA11850.18470660485879686No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA11500.17925113551697588No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA11360.17706894778024745No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA10360.16148189251790174No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA10330.16101428086003136No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA9610.14979160107114242No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA9530.14854463665015477No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC9350.14573896670293254No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAAT8780.13685434520339548No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT8740.13623086299290166No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA8630.13451628691404363No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA8230.12828146480910535No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG8170.1273462414933646No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA8040.12531992430925964No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8030.1251640537566362No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG7970.12422883044089544No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA7890.12298186601990778No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC7670.11955271386219174No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7030.10957699849429046No Hit
CTGTAGAACATATTAGATGAGTGAGTTACACTGAAAAA6680.10412152915246946No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC6520.10162760031049414No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG6510.10147172975787068No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA6470.10084824754737687No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCC308.403938E-426.6714745
GGGCGCA350.002063387822.8612637
CATTTCG350.00206619522.85591714
TAGGACC8450.021.021544
ATACATG2350.020.426031
TGGGCGC400.00447668420.0036056
GGTTAAC400.00448274719.9989322
TAGGAAC659.408504E-619.6958584
AGCTGTA507.18248E-419.203467
CTCTAGG1600.019.0004631
TCCTCGC4900.018.93776129
TTCCTCG5200.018.76822528
CCTCGCC4950.018.74647130
GGACCTA1208.185452E-1118.6700336
AAAGACC601.13561575E-418.6700334
GTATAGG601.1369745E-418.667121
CTGCTTA601.1376544E-418.66566525
AATTTCG601.1376544E-418.66566518
CGGTTGG601.1376544E-418.66566523
TTCGGTT601.1376544E-418.66566521
Produced by FastQC (version 0.11.5)