##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062871_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	641558
Sequences flagged as poor quality	0
Sequence length	38
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.61838680212857	32.0	32.0	32.0	32.0	32.0
2	31.29577684324722	32.0	32.0	32.0	32.0	32.0
3	31.41566623750308	32.0	32.0	32.0	32.0	32.0
4	31.547079141714388	32.0	32.0	32.0	32.0	32.0
5	31.43334195817058	32.0	32.0	32.0	32.0	32.0
6	34.912595587616394	36.0	36.0	36.0	36.0	36.0
7	34.968213318203496	36.0	36.0	36.0	36.0	36.0
8	34.95291774087456	36.0	36.0	36.0	36.0	36.0
9	35.02264643259066	36.0	36.0	36.0	36.0	36.0
10	34.916498586254086	36.0	36.0	36.0	32.0	36.0
11	35.05926042540191	36.0	36.0	36.0	36.0	36.0
12	34.996757892505435	36.0	36.0	36.0	36.0	36.0
13	35.001476094133345	36.0	36.0	36.0	36.0	36.0
14	34.957952359724295	36.0	36.0	36.0	36.0	36.0
15	34.941154190268065	36.0	36.0	36.0	36.0	36.0
16	34.93187209885934	36.0	36.0	36.0	36.0	36.0
17	34.92087699007728	36.0	36.0	36.0	36.0	36.0
18	34.90047353473887	36.0	36.0	36.0	36.0	36.0
19	34.83353960203131	36.0	36.0	36.0	32.0	36.0
20	34.77904881553967	36.0	36.0	36.0	32.0	36.0
21	34.72964564388567	36.0	36.0	36.0	32.0	36.0
22	34.72037758082668	36.0	36.0	36.0	32.0	36.0
23	34.716574339342664	36.0	36.0	36.0	32.0	36.0
24	34.7041888652312	36.0	36.0	36.0	32.0	36.0
25	34.68684514884079	36.0	36.0	36.0	32.0	36.0
26	34.60951465027324	36.0	36.0	36.0	32.0	36.0
27	34.57450924156507	36.0	36.0	36.0	32.0	36.0
28	34.49860339984849	36.0	36.0	36.0	32.0	36.0
29	34.44147060748989	36.0	36.0	36.0	32.0	36.0
30	34.36395462296472	36.0	36.0	36.0	32.0	36.0
31	34.317502080871876	36.0	36.0	36.0	32.0	36.0
32	34.223063230448375	36.0	36.0	36.0	32.0	36.0
33	34.13509300795875	36.0	36.0	36.0	32.0	36.0
34	34.02230039996384	36.0	36.0	36.0	32.0	36.0
35	33.91258935279429	36.0	36.0	36.0	32.0	36.0
36	33.80843976694235	36.0	36.0	36.0	32.0	36.0
37	33.71829203283257	36.0	36.0	36.0	27.0	36.0
38	33.32360441300709	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	0.0
19	2.0
20	13.0
21	16.0
22	58.0
23	158.0
24	425.0
25	1179.0
26	2414.0
27	4947.0
28	8831.0
29	14262.0
30	21372.0
31	29684.0
32	41129.0
33	62348.0
34	138548.0
35	316171.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.07725163327882	20.703631015384232	14.998729542120746	21.2203878092162
2	10.427584099956668	24.056281739141276	46.84143912163826	18.674695039263792
3	18.63946829437089	31.78340851489655	31.15010645958744	18.42701673114512
4	9.344327454388166	19.604866301408297	38.95940332473443	32.091402919469104
5	8.586285261815767	43.416339598290406	34.619473219880355	13.377901920013468
6	25.515737494875744	40.48775089352933	20.434817171350325	13.561694440244592
7	22.445932149231165	35.19900865864969	23.58098682108315	18.774072371036
8	24.429414497127198	39.52370412168334	21.152892909806805	14.893988471382654
9	27.434643604807952	14.213252566946366	22.074569655552974	36.27753417269271
10	15.46112468065672	31.566625256604695	33.94601520377959	19.02623485895899
11	31.380333766675083	22.94217225802228	27.837219380040718	17.840274595261924
12	24.504288811680407	27.31715072644037	34.80367171535321	13.37488874652601
13	31.748698868533893	24.071750444621447	28.006396937450607	16.173153749394054
14	20.475467533722593	25.444309010253164	31.323434514104726	22.756788941919513
15	20.34360104620315	35.74594970369008	28.94968186820209	14.960767381904677
16	16.007126401665943	28.942044211123548	36.54525389754317	18.505575489667343
17	15.792025039045573	30.578217402011976	33.80723177015952	19.822525788782933
18	16.163620436499894	26.478665997462425	41.90361588507976	15.454097680957918
19	21.335249501993584	25.919870066307332	34.456744362941464	18.28813606875762
20	21.699207242369354	27.089990304851625	37.05526234572712	14.155540107051895
21	21.33356817866534	25.234546579649198	32.440297588522924	20.99158765316254
22	20.51646148906256	30.04264618319778	33.5034400630964	15.937452264643259
23	17.806807802256383	30.18729405603235	35.0869290071981	16.91896913451317
24	19.84715333609744	29.196736694110275	35.807206830871095	15.148903138921188
25	19.286205278720363	28.976380898345745	35.847789050700094	15.889624772233802
26	14.608499932975663	30.618743745694076	38.4213118689191	16.35144445241116
27	16.505288687850513	30.643838904666453	36.93508614965443	15.915786257828598
28	15.49431228353477	31.275893995554572	34.912977470470324	18.316816250440336
29	16.771827332836626	28.367505354153483	35.00260303822881	19.85806427478108
30	16.064019153373508	29.683052818295465	36.0218405818336	18.231087446497433
31	18.30933446391441	28.10938371900904	35.49250418512434	18.088777631952215
32	15.69180027370869	31.97419407130766	35.66910552124671	16.664900133736936
33	15.548711106400356	31.605248473247936	36.9235517287603	15.922488691591408
34	16.03845638274326	31.86321423783976	37.08690406790968	15.011425311507299
35	16.030506984559466	32.68028767469192	35.6889010814299	15.60030425931872
36	15.237281742258691	32.45801626665087	36.14108155459054	16.163620436499894
37	15.999332874034772	32.38880974128605	33.27960994952912	18.332247435150055
38	17.09840279195769	34.36545778473308	32.57309950635719	15.96303991695204
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1320.0
1	995.5
2	671.0
3	671.0
4	2340.5
5	4010.0
6	4010.0
7	4732.0
8	5454.0
9	5491.0
10	5528.0
11	5528.0
12	6616.5
13	7705.0
14	8704.0
15	9703.0
16	9703.0
17	14495.5
18	19288.0
19	19288.0
20	20156.0
21	21024.0
22	19358.5
23	17693.0
24	17693.0
25	19319.0
26	20945.0
27	20945.0
28	28594.5
29	36244.0
30	46101.0
31	55958.0
32	55958.0
33	60882.0
34	65806.0
35	65806.0
36	65884.5
37	65963.0
38	65829.5
39	65696.0
40	65696.0
41	59010.0
42	52324.0
43	46775.0
44	41226.0
45	41226.0
46	45461.0
47	49696.0
48	49696.0
49	42591.5
50	35487.0
51	27018.0
52	18549.0
53	18549.0
54	15141.5
55	11734.0
56	11734.0
57	10211.0
58	8688.0
59	7532.5
60	6377.0
61	6377.0
62	5589.5
63	4802.0
64	4066.5
65	3331.0
66	3331.0
67	2875.0
68	2419.0
69	2419.0
70	2041.5
71	1664.0
72	1354.0
73	1044.0
74	1044.0
75	837.5
76	631.0
77	631.0
78	489.5
79	348.0
80	233.5
81	119.0
82	119.0
83	81.5
84	44.0
85	44.0
86	34.5
87	25.0
88	18.0
89	11.0
90	11.0
91	11.0
92	11.0
93	8.5
94	6.0
95	6.0
96	7.5
97	9.0
98	9.0
99	7.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008884621499537065
2	0.0
3	0.0
4	4.6761165787037183E-4
5	0.0
6	1.5587055262345729E-4
7	4.6761165787037183E-4
8	0.0037408932629629747
9	0.005767210447067919
10	0.0010910938683642009
11	0.004987857683950633
12	7.793527631172864E-4
13	1.5587055262345729E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.5587055262345729E-4
22	0.0
23	0.0
24	0.0
25	1.5587055262345729E-4
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.5587055262345729E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	641558.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.23767520328988
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.99515776140261	27.529274586901277
2	14.889242162328337	11.38660011652481
3	5.2955607426493545	6.074697950901558
4	2.503029942166763	3.8284018381072857
5	1.3449266657376484	2.5713434508359914
6	0.8143317794538295	1.8682892454283575
7	0.5315695192066355	1.4228187836374606
8	0.3896569829826583	1.1919661724787793
9	0.2828573113115637	0.9734225398926686
>10	1.6263707456151157	11.706893877997492
>50	0.16792790976420022	4.479547345572101
>100	0.13246740447292146	9.87005962447653
>500	0.01548849652298774	4.0437803694463526
>1k	0.010189800344070882	7.726915253079908
>5k	4.0759201376283524E-4	1.1533174567911435
>10k+	8.151840275256705E-4	4.172671387928282
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	14878	2.3190420819317974	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	11895	1.854080223456024	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	7400	1.1534420894135837	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4984	0.7768588342753111	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	3767	0.5871643717325635	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	3607	0.5622250833128104	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	3553	0.5538080734711437	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2211	0.34462979185046405	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2128	0.3316925359827171	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA	2087	0.3253018433251553	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2071	0.32280791448318	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG	2020	0.3148585162993837	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	1929	0.30067429601064904	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	1927	0.30036255490540215	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	1756	0.27370869040679097	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	1722	0.2684090916175934	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA	1701	0.2651358100125008	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	1680	0.2618625284074082	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	1557	0.24269045043472298	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	1403	0.21868638533071053	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG	1371	0.21369852764675992	No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAAT	1297	0.20216410675262408	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	1267	0.19748799017392038	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAA	1185	0.18470660485879686	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	1150	0.17925113551697588	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	1136	0.17706894778024745	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	1036	0.16148189251790174	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA	1033	0.16101428086003136	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA	961	0.14979160107114242	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA	953	0.14854463665015477	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	935	0.14573896670293254	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAAT	878	0.13685434520339548	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT	874	0.13623086299290166	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA	863	0.13451628691404363	No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA	823	0.12828146480910535	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	817	0.1273462414933646	No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA	804	0.12531992430925964	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	803	0.1251640537566362	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	797	0.12422883044089544	No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	789	0.12298186601990778	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	767	0.11955271386219174	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	703	0.10957699849429046	No Hit
CTGTAGAACATATTAGATGAGTGAGTTACACTGAAAAA	668	0.10412152915246946	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	652	0.10162760031049414	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	651	0.10147172975787068	No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA	647	0.10084824754737687	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	4.6761165787037183E-4	0.0
12	0.0	0.0	0.0	4.6761165787037183E-4	0.0
13	0.0	0.0	0.0	4.6761165787037183E-4	0.0
14	0.0	0.0	0.0	4.6761165787037183E-4	0.0
15	0.0	0.0	0.0	4.6761165787037183E-4	0.0
16	0.0	0.0	0.0	7.793527631172864E-4	0.0
17	0.0	0.0	0.0	0.0010910938683642009	0.0
18	0.0	0.0	0.0	0.0010910938683642009	0.0
19	0.0	0.0	0.0	0.0010910938683642009	0.0
20	0.0	0.0	0.0	0.0014028349736111155	0.0
21	0.0	0.0	0.0	0.002961540499845688	0.0
22	0.0	0.0	0.0	0.004676116578703718	0.0
23	0.0	0.0	0.0	0.009352233157407436	0.0
24	0.0	0.0	0.0	0.01667814913070993	0.0
25	0.0	0.0	0.0	0.020263171841049445	0.0
26	0.0	0.0	0.0	0.024783417867129707	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGACCC	30	8.403938E-4	26.671474	5
GGGCGCA	35	0.0020633878	22.861263	7
CATTTCG	35	0.002066195	22.855917	14
TAGGACC	845	0.0	21.02154	4
ATACATG	235	0.0	20.42603	1
TGGGCGC	40	0.004476684	20.003605	6
GGTTAAC	40	0.004482747	19.99893	22
TAGGAAC	65	9.408504E-6	19.695858	4
AGCTGTA	50	7.18248E-4	19.20346	7
CTCTAGG	160	0.0	19.000463	1
TCCTCGC	490	0.0	18.937761	29
TTCCTCG	520	0.0	18.768225	28
CCTCGCC	495	0.0	18.746471	30
GGACCTA	120	8.185452E-11	18.670033	6
AAAGACC	60	1.13561575E-4	18.670033	4
GTATAGG	60	1.1369745E-4	18.66712	1
CTGCTTA	60	1.1376544E-4	18.665665	25
AATTTCG	60	1.1376544E-4	18.665665	18
CGGTTGG	60	1.1376544E-4	18.665665	23
TTCGGTT	60	1.1376544E-4	18.665665	21
>>END_MODULE