FastQCFastQC Report
Thu 2 Feb 2017
SRR4062870_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062870_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences760464
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT28390.37332470702097664No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT19740.2595783626838351No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT12170.16003387405583958No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC11480.15096046624166298No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA11190.14714700498642933No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC11090.1458320183466936No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG11070.14556902101874647No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT11020.14491152769887858No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG10440.1372846051884113No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG10220.13439163458099265No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA10120.13307664794125693No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC9950.13084117065370615No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG9890.1300521786698647No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG8840.11624481895263943No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA8620.11335184834522082No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8600.11308885101727366No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT8470.11137936838561721No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT8380.11019588040985505No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA8270.10874939510614572No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG8230.10822340045025143No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA7930.10427844053104421No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT7680.10099097393170486No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGT350.002066863722.85531223
GTGTAAA851.0410076E-820.730121
TGTCGCA400.00448418719.99839832
ATACCGT2050.018.7289756
TACCGTC2150.018.603167
CCGTCGT2100.018.285459
CGTCGTA2100.018.2854510
ATCGTTT2100.018.28424829
ACCGTCG2100.018.2842488
GGCTAGA450.0087881617.798591
CGAAAAA1352.3646862E-1117.77635424
TCTAGCG1900.017.68279528
GTCCTAA1006.7147994E-817.6206041
ACGTATG550.00136480317.45314622
GTCGTAG2300.017.39105411
AACGTAT652.100512E-417.22938721
AACCGCG1404.0017767E-1117.1414837
TCTTATA753.215387E-517.0765292
TATACTA753.235415E-517.0641775
GTAGGAC3200.017.003113
Produced by FastQC (version 0.11.5)