##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062870_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	760464
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.17706295104042	32.0	32.0	32.0	32.0	32.0
2	30.791992783353322	32.0	32.0	32.0	32.0	32.0
3	30.84723405710198	32.0	32.0	32.0	32.0	32.0
4	30.903493130509794	32.0	32.0	32.0	32.0	32.0
5	30.83579498832292	32.0	32.0	32.0	32.0	32.0
6	34.482615876622695	36.0	36.0	36.0	32.0	36.0
7	34.38628784531549	36.0	36.0	36.0	32.0	36.0
8	34.36392649750679	36.0	36.0	36.0	32.0	36.0
9	34.48891071766711	36.0	36.0	36.0	32.0	36.0
10	34.21206263544362	36.0	36.0	36.0	32.0	36.0
11	34.51053830293084	36.0	36.0	36.0	32.0	36.0
12	34.29630856950494	36.0	36.0	36.0	32.0	36.0
13	34.39263791579877	36.0	36.0	36.0	32.0	36.0
14	34.30021276483831	36.0	36.0	36.0	32.0	36.0
15	34.23673309979171	36.0	36.0	36.0	32.0	36.0
16	34.258743346167606	36.0	36.0	36.0	32.0	36.0
17	34.16368559195438	36.0	36.0	36.0	32.0	36.0
18	34.20964700498643	36.0	36.0	36.0	32.0	36.0
19	34.1906967325212	36.0	36.0	36.0	32.0	36.0
20	34.15939084558901	36.0	36.0	36.0	32.0	36.0
21	34.12596520019357	36.0	36.0	36.0	32.0	36.0
22	34.11897341623009	36.0	36.0	36.0	32.0	36.0
23	34.06492615035031	36.0	36.0	36.0	32.0	36.0
24	34.03895516421553	36.0	36.0	36.0	32.0	36.0
25	34.02129489364388	36.0	36.0	36.0	32.0	36.0
26	33.98835842327842	36.0	36.0	36.0	32.0	36.0
27	33.9812890551032	36.0	36.0	36.0	32.0	36.0
28	33.954045161901156	36.0	36.0	36.0	32.0	36.0
29	33.93047008142397	36.0	36.0	36.0	32.0	36.0
30	33.88705711249974	36.0	36.0	36.0	32.0	36.0
31	33.90807191398935	36.0	36.0	36.0	32.0	36.0
32	33.85230727555808	36.0	36.0	36.0	32.0	36.0
33	33.819586463001535	36.0	36.0	36.0	32.0	36.0
34	33.81409770876728	36.0	36.0	36.0	32.0	36.0
35	33.769261924298846	36.0	36.0	36.0	32.0	36.0
36	33.70403069704812	36.0	36.0	36.0	27.0	36.0
37	33.712755633402764	36.0	36.0	36.0	27.0	36.0
38	33.094920995602685	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	1.0
8	0.0
9	0.0
10	8.0
11	7.0
12	6.0
13	2.0
14	101.0
15	225.0
16	269.0
17	360.0
18	489.0
19	612.0
20	775.0
21	1075.0
22	1651.0
23	2532.0
24	3706.0
25	5637.0
26	8208.0
27	11609.0
28	15987.0
29	21354.0
30	28320.0
31	37388.0
32	50613.0
33	75017.0
34	163697.0
35	330813.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.46500635912884	17.441392004086694	11.443140435369923	25.650461201414544
2	16.347036889872456	20.390213223936446	38.026119471716186	25.236630414474913
3	18.828687952170085	24.483238882583404	29.060932898978248	27.627140266268256
4	12.233264031623673	15.64758962867697	36.477684717986754	35.64146162171261
5	14.31205158955585	36.89523764438553	33.590544720065644	15.202166045992973
6	33.19451439452018	36.255807317384274	17.21385524345037	13.33582304464517
7	29.30228032133056	31.00755723815623	21.23140770819882	18.458754732314393
8	27.746890040238803	33.172947952555035	19.988165057991218	19.09199694921495
9	27.69517437449126	14.011106420973594	18.876576182079027	39.417143022456116
10	15.98898562845432	26.741673800497328	31.398717356971627	25.870623214076726
11	37.661565732876234	21.186956653240387	22.340617188003403	18.810860425879973
12	24.494704423456763	24.256817709607255	29.12133142918761	22.127146437748372
13	29.73834934538018	19.326639179867563	25.503935609871576	25.431075864880683
14	23.53030912758845	20.3740672647745	25.274527287026004	30.821096320611048
15	25.179699984874294	27.541020261872035	22.804569279029852	24.47471047422382
16	25.332132195320824	25.85120793277134	24.65129736056497	24.165362511342863
17	23.4619780589785	25.94004295610612	25.88572307910842	24.712255905806966
18	24.652760426392575	24.69536812260496	27.312322303550907	23.33954914745156
19	25.291780694013944	24.91198907967602	25.91841055631318	23.877819669996857
20	25.35532621102853	24.436370044238245	25.726169999000565	24.482133745732668
21	27.239288814076428	24.291049683069883	24.509613108545278	23.96004839430841
22	25.465307577086605	24.596303840949172	25.557626073268274	24.380762508695955
23	23.983950467914088	24.212515583985187	25.941614632375764	25.86191931572496
24	24.29435669476702	25.604902876231307	25.67315911990215	24.427581309099516
25	24.810348999926347	24.476158159990742	25.833701587702407	24.8797912523805
26	23.90137531121069	25.986897915003475	26.542309528538006	23.56941724524783
27	24.865860779269966	25.006312433916705	25.661358961815033	24.46646782499829
28	23.759677128771074	25.29328916516641	26.291018072740712	24.656015633321804
29	23.703086080417474	25.013874605132685	26.2270805989621	25.05595871548774
30	23.709632029793198	25.718595238032616	26.486832657848986	24.084940074325196
31	24.69530156859857	25.201861066983682	25.07153903611669	25.031298328301055
32	24.009521116758613	25.492921628321376	25.223726517756752	25.273830737163262
33	23.81171562973844	24.780280982584234	26.037772076122796	25.37023131155453
34	24.522703869264852	25.359262576327268	26.18727289226265	23.930760662145225
35	25.459521592517188	25.074964884869722	26.04806169741542	23.41745182519767
36	23.7714027189788	26.096094081874483	25.343554820814905	24.788948378331817
37	24.874440918517777	25.89982364521784	25.337618802760133	23.888116633504254
38	24.178751965998096	25.34059766342113	25.916852088560656	24.563798282020123
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	515.0
1	373.0
2	231.0
3	231.0
4	517.5
5	804.0
6	804.0
7	969.0
8	1134.0
9	1069.0
10	1004.0
11	1004.0
12	1271.5
13	1539.0
14	1941.5
15	2344.0
16	2344.0
17	3411.5
18	4479.0
19	4479.0
20	5285.5
21	6092.0
22	6327.5
23	6563.0
24	6563.0
25	8138.5
26	9714.0
27	9714.0
28	12465.5
29	15217.0
30	18280.5
31	21344.0
32	21344.0
33	25919.0
34	30494.0
35	30494.0
36	33075.0
37	35656.0
38	40211.5
39	44767.0
40	44767.0
41	46124.5
42	47482.0
43	53025.5
44	58569.0
45	58569.0
46	59674.5
47	60780.0
48	60780.0
49	63956.5
50	67133.0
51	67394.5
52	67656.0
53	67656.0
54	63444.0
55	59232.0
56	59232.0
57	57600.0
58	55968.0
59	49869.5
60	43771.0
61	43771.0
62	42454.0
63	41137.0
64	34058.5
65	26980.0
66	26980.0
67	22689.5
68	18399.0
69	18399.0
70	15022.5
71	11646.0
72	9137.5
73	6629.0
74	6629.0
75	5061.5
76	3494.0
77	3494.0
78	3465.5
79	3437.0
80	2724.0
81	2011.0
82	2011.0
83	2067.0
84	2123.0
85	2123.0
86	1446.5
87	770.0
88	651.0
89	532.0
90	532.0
91	377.5
92	223.0
93	187.5
94	152.0
95	152.0
96	133.0
97	114.0
98	114.0
99	221.5
100	329.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12176776283952956
2	0.05496644154095394
3	0.013544362389278124
4	0.0027614719434450544
5	0.0
6	1.3149866397357403E-4
7	1.3149866397357403E-4
8	5.259946558942961E-4
9	3.944959919207221E-4
10	3.944959919207221E-4
11	0.0019724799596036106
12	0.0023669759515243323
13	0.013018367733383829
14	0.008678911822255886
15	0.02222327421153401
16	0.009730901134044477
17	0.020119295587956824
18	0.004996949230995813
19	0.006180437206757979
20	0.004733951903048665
21	0.0057859412148372575
22	0.0069694291905994235
23	0.008941909150203034
24	0.012360874413515958
25	0.015779839676828885
26	0.0167003303246439
27	0.007889919838414442
28	0.0048654505670222385
29	0.01078289044583307
30	0.0028929706074186288
31	0.005259946558942961
32	0.006443434534705128
33	0.008021418502388016
34	0.01144038376570094
35	0.014201855709145995
36	0.013807359717225274
37	0.008547413158282311
38	0.004733951903048665
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	760464.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.230522201237434
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.29868374883917	46.83928485872956
2	10.806097004525448	12.15264955043387
3	2.6853365086789194	4.529936225071902
4	1.0481596906488335	2.3575426702188556
5	0.49107124031111027	1.3806596140351546
6	0.30955271664393974	1.0443786543420248
7	0.2139305659006952	0.8420599204781631
8	0.14914273839264489	0.6709099249872772
9	0.11450585928330963	0.579485183634172
>10	0.6654441841367196	7.386866327436303
>50	0.09543759161810628	3.8533332088538104
>100	0.11091366239591642	12.867316669497972
>500	0.009145101127619008	3.5582342693176896
>1k	0.0025793874975335663	1.9373429229631964
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2839	0.37332470702097664	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1974	0.2595783626838351	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1217	0.16003387405583958	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1148	0.15096046624166298	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1119	0.14714700498642933	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1109	0.1458320183466936	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1107	0.14556902101874647	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1102	0.14491152769887858	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1044	0.1372846051884113	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1022	0.13439163458099265	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1012	0.13307664794125693	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	995	0.13084117065370615	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	989	0.1300521786698647	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	884	0.11624481895263943	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	862	0.11335184834522082	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	860	0.11308885101727366	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	847	0.11137936838561721	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	838	0.11019588040985505	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	827	0.10874939510614572	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	823	0.10822340045025143	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	793	0.10427844053104421	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	768	0.10099097393170486	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3149866397357403E-4
2	0.0	0.0	0.0	0.0	1.3149866397357403E-4
3	0.0	0.0	0.0	0.0	1.3149866397357403E-4
4	0.0	0.0	0.0	0.0	1.3149866397357403E-4
5	0.0	0.0	0.0	0.0	1.3149866397357403E-4
6	0.0	0.0	0.0	0.0	1.3149866397357403E-4
7	0.0	0.0	0.0	0.0	1.3149866397357403E-4
8	0.0	0.0	0.0	0.0	2.6299732794714806E-4
9	0.0	0.0	0.0	0.0	2.6299732794714806E-4
10	0.0	0.0	0.0	0.0	2.6299732794714806E-4
11	0.0	0.0	0.0	0.0	2.6299732794714806E-4
12	0.0	0.0	0.0	0.0	6.574933198678701E-4
13	0.0	0.0	0.0	1.3149866397357403E-4	6.574933198678701E-4
14	0.0	0.0	0.0	1.3149866397357403E-4	6.574933198678701E-4
15	0.0	0.0	0.0	3.9449599192072207E-4	7.889919838414441E-4
16	0.0	0.0	0.0	3.9449599192072207E-4	7.889919838414441E-4
17	0.0	0.0	1.3149866397357403E-4	3.9449599192072207E-4	7.889919838414441E-4
18	0.0	0.0	1.3149866397357403E-4	5.259946558942961E-4	7.889919838414441E-4
19	0.0	0.0	1.3149866397357403E-4	5.259946558942961E-4	9.204906478150181E-4
20	0.0	0.0	1.3149866397357403E-4	7.889919838414441E-4	9.204906478150181E-4
21	0.0	0.0	1.3149866397357403E-4	9.204906478150181E-4	9.204906478150181E-4
22	0.0	0.0	1.3149866397357403E-4	0.0013149866397357403	9.204906478150181E-4
23	0.0	0.0	1.3149866397357403E-4	0.0024984746154979066	9.204906478150181E-4
24	0.0	0.0	1.3149866397357403E-4	0.003418965263312925	9.204906478150181E-4
25	0.0	0.0	1.3149866397357403E-4	0.004076458583180795	9.204906478150181E-4
26	0.0	0.0	1.3149866397357403E-4	0.005128447894969387	9.204906478150181E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGT	35	0.0020668637	22.855312	23
GTGTAAA	85	1.0410076E-8	20.73012	1
TGTCGCA	40	0.004484187	19.998398	32
ATACCGT	205	0.0	18.728975	6
TACCGTC	215	0.0	18.60316	7
CCGTCGT	210	0.0	18.28545	9
CGTCGTA	210	0.0	18.28545	10
ATCGTTT	210	0.0	18.284248	29
ACCGTCG	210	0.0	18.284248	8
GGCTAGA	45	0.00878816	17.79859	1
CGAAAAA	135	2.3646862E-11	17.776354	24
TCTAGCG	190	0.0	17.682795	28
GTCCTAA	100	6.7147994E-8	17.620604	1
ACGTATG	55	0.001364803	17.453146	22
GTCGTAG	230	0.0	17.391054	11
AACGTAT	65	2.100512E-4	17.229387	21
AACCGCG	140	4.0017767E-11	17.141483	7
TCTTATA	75	3.215387E-5	17.076529	2
TATACTA	75	3.235415E-5	17.064177	5
GTAGGAC	320	0.0	17.00311	3
>>END_MODULE