FastQCFastQC Report
Thu 2 Feb 2017
SRR4062870_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062870_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences760464
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT31350.4122483115571546No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT24240.3187527614719435No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT13780.181205158955585No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG11810.15529992215279093No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC11700.15385343684908162No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA11350.14925098361000652No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT11340.14911948494603294No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC10920.14359654105914285No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC10820.1422815544194071No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG10260.13491762923688694No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA10160.1336026425971512No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA10100.13281365061330977No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG9800.12886869069410256No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9780.1286056933661554No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG9780.1286056933661554No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT9310.12242525615939741No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG9230.12137326684760882No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC8870.11663931494456017No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG8850.11637631761661302No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC8840.11624481895263943No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG8640.11361484567316796No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA8250.10848639777819857No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG8120.1067769151465421No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT7960.10467293652296492No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA7880.10362094721117633No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT7740.1017799659155463No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCAA350.00206530922.8582710
TAATACC502.7963557E-522.3996334
CTACACG400.004482534219.9996724
AACCGCG1800.018.6675877
GTATTAG2050.017.9521051
GACTAAG550.001363619517.4554061
GCATTAC550.001364220517.4542613
TAGCGGC1850.017.29701230
TCTAGCG1850.017.29701228
CTAGCGG1900.016.84182729
AGAACCG2200.016.7280985
CGTGAAA1252.8467184E-916.640829
CGCGGTC2050.016.39105210
GAACCGC2150.016.37296
GTCCTAC2450.016.3273351
TACGACG3250.016.2469545
CGACGGT3300.016.000797
GACGGTA3300.016.000798
GTTATAT600.002440082116.000791
TTGGACT805.6176366E-515.9997374
Produced by FastQC (version 0.11.5)