##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062870_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	760464
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.17963637989438	32.0	32.0	32.0	32.0	32.0
2	31.322664846725157	32.0	32.0	32.0	32.0	32.0
3	31.39820556923142	32.0	32.0	32.0	32.0	32.0
4	31.489315733552147	32.0	32.0	32.0	32.0	32.0
5	31.42372288497549	32.0	32.0	32.0	32.0	32.0
6	34.9497280607629	36.0	36.0	36.0	36.0	36.0
7	34.96770655810137	36.0	36.0	36.0	36.0	36.0
8	34.91406167813335	36.0	36.0	36.0	36.0	36.0
9	35.00914441709272	36.0	36.0	36.0	36.0	36.0
10	34.868105525047866	36.0	36.0	36.0	32.0	36.0
11	35.03861852763576	36.0	36.0	36.0	36.0	36.0
12	34.92760341054935	36.0	36.0	36.0	32.0	36.0
13	34.978911559258556	36.0	36.0	36.0	36.0	36.0
14	34.92711555050601	36.0	36.0	36.0	32.0	36.0
15	34.90921989732584	36.0	36.0	36.0	32.0	36.0
16	34.91145668960004	36.0	36.0	36.0	32.0	36.0
17	34.87902517410423	36.0	36.0	36.0	32.0	36.0
18	34.87140219655368	36.0	36.0	36.0	32.0	36.0
19	34.86504160617728	36.0	36.0	36.0	32.0	36.0
20	34.84565212817438	36.0	36.0	36.0	32.0	36.0
21	34.82289759936039	36.0	36.0	36.0	32.0	36.0
22	34.81443040038713	36.0	36.0	36.0	32.0	36.0
23	34.74682825222496	36.0	36.0	36.0	32.0	36.0
24	34.71690967619769	36.0	36.0	36.0	32.0	36.0
25	34.7048275789518	36.0	36.0	36.0	32.0	36.0
26	34.64279571419554	36.0	36.0	36.0	32.0	36.0
27	34.62743667024343	36.0	36.0	36.0	32.0	36.0
28	34.588448631361906	36.0	36.0	36.0	32.0	36.0
29	34.548334963916766	36.0	36.0	36.0	32.0	36.0
30	34.505053493656504	36.0	36.0	36.0	32.0	36.0
31	34.5164110332639	36.0	36.0	36.0	32.0	36.0
32	34.48478954953818	36.0	36.0	36.0	32.0	36.0
33	34.44027593679648	36.0	36.0	36.0	32.0	36.0
34	34.42694723221612	36.0	36.0	36.0	32.0	36.0
35	34.38757653222243	36.0	36.0	36.0	32.0	36.0
36	34.354822844999894	36.0	36.0	36.0	32.0	36.0
37	34.33975178312188	36.0	36.0	36.0	32.0	36.0
38	33.90289875654863	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	3.0
21	10.0
22	55.0
23	154.0
24	490.0
25	1237.0
26	2622.0
27	5146.0
28	9078.0
29	14242.0
30	21195.0
31	30948.0
32	44140.0
33	70211.0
34	169595.0
35	391338.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.92496501804332	18.69601468716136	12.198313501457143	26.18070679333817
2	15.376007279766037	21.405221022954407	37.335363672705085	25.88340802457447
3	17.909197542552967	25.170816764501673	29.47989648425172	27.440089208693642
4	11.757867924850848	16.42122344209631	36.51679704810635	35.304111584946504
5	13.678622525195145	37.49421405878516	33.35897557280818	15.468187843211512
6	33.189210797618294	36.782543289360184	16.745697363714783	13.282548549306739
7	28.94714002803559	31.3141222046598	21.3134910094127	18.425246757891912
8	27.675849825761063	33.309093300019725	19.88349003879282	19.13156683542639
9	27.645914729487515	13.731495593922888	18.696074705395784	39.92651497119382
10	15.624946578176019	27.08450313429951	31.640842282995617	25.649708004528854
11	37.783192799920045	20.99900583349552	22.328243270965604	18.88955809561883
12	24.221264911948495	24.401549580256265	29.10946474783816	22.26772075995708
13	29.66820257106188	19.35186938500705	25.51968272002356	25.46024532390751
14	23.41767657640598	20.29892276294473	25.371352227061372	30.912048433587913
15	25.017094826316566	27.516489932462285	22.890761429863872	24.575653811357277
16	25.276673189000398	25.920622146478994	24.55553451576932	24.247170148751287
17	23.469355551347597	25.97453659870816	25.83554251088809	24.720565339056154
18	24.641797639335987	24.639693660712407	27.373945380714932	23.344563319236677
19	25.390156536009595	24.88349218371941	25.90076584821898	23.82558543205201
20	25.295871993940544	24.312393486082183	25.83186054829683	24.55987397168045
21	27.211281533379623	24.306213048875424	24.50004207957247	23.982463338172487
22	25.24826947758211	24.51542742325738	25.671695175577014	24.564607923583495
23	23.854283508862366	24.038381880512265	26.0816108081524	26.025723802472967
24	24.333564770981926	25.5209977066633	25.779392581371376	24.3660449409834
25	24.780824313608946	24.520851113071902	25.875157634230728	24.823166939088424
26	23.90211765448463	25.72337415051863	26.70225020513792	23.67225798985882
27	24.988691114898273	24.66480990553136	25.80437732752635	24.542121652044017
28	23.702897178564665	25.001972479959605	26.649650739548488	24.645479601927246
29	23.73537734856614	24.751204527761995	26.421632056218307	25.091786067453555
30	23.719597508889308	25.417771255444045	26.77457447032338	24.088056765343264
31	24.656525489701025	24.909660417850155	25.293373519325048	25.14044057312378
32	24.011656041574618	25.244456016326872	25.39949294115172	25.34439500094679
33	23.942487744324517	24.480711775968356	26.167313640093415	25.40948683961371
34	24.535020724189444	24.952265984977593	26.501977739906163	24.010735550926803
35	25.58359107071472	24.865608365419007	26.09801384417934	23.452786719686927
36	23.970759957078837	25.764270235014415	25.56991520966147	24.69505459824528
37	25.01104588777378	25.528230133181847	25.448804940141805	24.011919038902565
38	24.20094626687461	25.121176337542177	26.075070154721736	24.602807240861477
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	266.0
1	214.5
2	163.0
3	163.0
4	471.0
5	779.0
6	779.0
7	922.0
8	1065.0
9	1042.5
10	1020.0
11	1020.0
12	1328.5
13	1637.0
14	1988.5
15	2340.0
16	2340.0
17	3594.5
18	4849.0
19	4849.0
20	5552.0
21	6255.0
22	6577.5
23	6900.0
24	6900.0
25	8200.5
26	9501.0
27	9501.0
28	12312.5
29	15124.0
30	18119.0
31	21114.0
32	21114.0
33	26105.5
34	31097.0
35	31097.0
36	33291.5
37	35486.0
38	40035.5
39	44585.0
40	44585.0
41	46026.0
42	47467.0
43	53114.5
44	58762.0
45	58762.0
46	59976.5
47	61191.0
48	61191.0
49	64547.0
50	67903.0
51	67537.5
52	67172.0
53	67172.0
54	63340.5
55	59509.0
56	59509.0
57	57855.5
58	56202.0
59	50033.0
60	43864.0
61	43864.0
62	42592.0
63	41320.0
64	34048.0
65	26776.0
66	26776.0
67	22644.5
68	18513.0
69	18513.0
70	15084.5
71	11656.0
72	9168.5
73	6681.0
74	6681.0
75	5039.5
76	3398.0
77	3398.0
78	3356.5
79	3315.0
80	2608.0
81	1901.0
82	1901.0
83	1844.5
84	1788.0
85	1788.0
86	1125.0
87	462.0
88	389.0
89	316.0
90	316.0
91	184.0
92	52.0
93	33.5
94	15.0
95	15.0
96	11.0
97	7.0
98	7.0
99	10.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00946790380609733
2	0.0
3	0.0
4	3.944959919207221E-4
5	0.0
6	0.0
7	2.6299732794714806E-4
8	0.0018409812956300365
9	0.0048654505670222385
10	9.204906478150183E-4
11	0.003681962591260073
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	1.3149866397357403E-4
24	0.0
25	1.3149866397357403E-4
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	2.6299732794714806E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	760464.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.97992553036302
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.93325454227195	44.227509786521175
2	11.59038117606607	12.512958255051357
3	2.9913645352025098	4.844209045332013
4	1.1230385875093059	2.424861572859056
5	0.5659466064915729	1.5274877836288383
6	0.33814320688615856	1.0951767075787786
7	0.22445577330096741	0.8481274149352384
8	0.15343825476540163	0.6626068452596198
9	0.11312401019008483	0.5495783081181125
>10	0.7293936555900201	7.685278575482577
>50	0.10306250438028558	3.950474160817436
>100	0.1214225593507121	13.670773139881149
>500	0.010036945430072252	3.7831726892324453
>1k	0.0029376425648991963	2.2177857153022504
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3135	0.4122483115571546	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2424	0.3187527614719435	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1378	0.181205158955585	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1181	0.15529992215279093	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1170	0.15385343684908162	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1135	0.14925098361000652	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1134	0.14911948494603294	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1092	0.14359654105914285	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1082	0.1422815544194071	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1026	0.13491762923688694	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1016	0.1336026425971512	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1010	0.13281365061330977	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	980	0.12886869069410256	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	978	0.1286056933661554	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	978	0.1286056933661554	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	931	0.12242525615939741	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	923	0.12137326684760882	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	887	0.11663931494456017	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	885	0.11637631761661302	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	884	0.11624481895263943	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	864	0.11361484567316796	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	825	0.10848639777819857	No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG	812	0.1067769151465421	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	796	0.10467293652296492	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	788	0.10362094721117633	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	774	0.1017799659155463	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	5.259946558942961E-4
13	0.0	0.0	0.0	1.3149866397357403E-4	6.574933198678701E-4
14	0.0	0.0	0.0	1.3149866397357403E-4	6.574933198678701E-4
15	0.0	0.0	0.0	2.6299732794714806E-4	7.889919838414441E-4
16	0.0	0.0	0.0	2.6299732794714806E-4	7.889919838414441E-4
17	0.0	0.0	0.0	2.6299732794714806E-4	7.889919838414441E-4
18	0.0	0.0	0.0	3.9449599192072207E-4	7.889919838414441E-4
19	0.0	0.0	0.0	3.9449599192072207E-4	7.889919838414441E-4
20	0.0	0.0	0.0	5.259946558942961E-4	0.0010519893117885923
21	0.0	0.0	0.0	5.259946558942961E-4	0.0014464853037093144
22	0.0	0.0	0.0	9.204906478150181E-4	0.0014464853037093144
23	0.0	0.0	0.0	0.0021039786235771845	0.0015779839676828883
24	0.0	0.0	0.0	0.0030244692713922027	0.0015779839676828883
25	0.0	0.0	0.0	0.003813461255233647	0.0015779839676828883
26	0.0	0.0	0.0	0.004996949230995813	0.0015779839676828883
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGCAA	35	0.002065309	22.85827	10
TAATACC	50	2.7963557E-5	22.399633	4
CTACACG	40	0.0044825342	19.999672	4
AACCGCG	180	0.0	18.667587	7
GTATTAG	205	0.0	17.952105	1
GACTAAG	55	0.0013636195	17.455406	1
GCATTAC	55	0.0013642205	17.45426	13
TAGCGGC	185	0.0	17.297012	30
TCTAGCG	185	0.0	17.297012	28
CTAGCGG	190	0.0	16.841827	29
AGAACCG	220	0.0	16.728098	5
CGTGAAA	125	2.8467184E-9	16.64082	9
CGCGGTC	205	0.0	16.391052	10
GAACCGC	215	0.0	16.3729	6
GTCCTAC	245	0.0	16.327335	1
TACGACG	325	0.0	16.246954	5
CGACGGT	330	0.0	16.00079	7
GACGGTA	330	0.0	16.00079	8
GTTATAT	60	0.0024400821	16.00079	1
TTGGACT	80	5.6176366E-5	15.999737	4
>>END_MODULE