FastQCFastQC Report
Thu 2 Feb 2017
SRR4062869_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062869_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences851266
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT33100.38883263280807645No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT20930.24586909379676858No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA19840.23306463549583795No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC19600.23024530522774314No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT18160.21332932361917425No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT17830.20945274450054388No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA16830.19770553505014882No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG15320.1799672487800523No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC15240.1790274720240207No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC14930.17538583709439823No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG14380.16892487189668096No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC14000.16446093230553083No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG13760.161641602037436No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC13740.16140665784842811No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG13660.16046688109239649No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA12740.14965944839803305No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT12480.1466051739409303No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG12310.14460814833436317No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC12140.14261112272779602No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG11780.1383821273256538No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG11710.13755982266412614No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA11610.13638510171908663No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT11480.13485796449053528No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC10880.12780963882029825No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT10490.12322822713464418No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT10150.11923417592150985No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT10120.11888175963799799No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT9920.11653231774791897No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA9920.11653231774791897No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG9780.11488770842486366No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA9650.11336057119631232No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG9520.11183343396776095No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9520.11183343396776095No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT9460.11112860140073724No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC9450.1110111293062333No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC9190.10795685484913059No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT9110.10701707809309899No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG8990.10560741295905157No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC8730.10255313850194886No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAATCG350.002066301222.85676417
AAGACGG4550.020.0389165
GACAATA400.00448601819.997327
CTAATTG400.004487543419.9961453
CAAGACG4450.019.7691464
CGCAAGA4450.019.4222562
GCGCAAG4600.018.8032881
CTAGCGG4600.018.78229729
GTTATTC2600.018.457983
TAGCGGC4600.018.43447730
CGCGTAA1650.018.42610410
AGAGCGA4750.018.1913115
TCTAGCG4750.018.18703728
GACGGAC4800.017.997597
GCAAGAC5350.017.9404663
CGCCGGT5000.017.9175997
CGTTATT2700.017.7837522
TAACGAA1900.017.68599713
AACGAAC2000.017.60177814
GTATAGA550.001354226817.4737631
Produced by FastQC (version 0.11.5)