FastQCFastQC Report
Thu 2 Feb 2017
SRR4062869_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062869_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences851266
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT36300.42642370304934063No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT25810.3031954759146965No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC21230.2493932566318871No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA19740.23188991455079846No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT18340.21544382132024537No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC18170.21344679571367822No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT16980.19946761646770808No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC16690.19606092572709352No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA16660.19570850944358167No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT15850.1861932697887617No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC15410.18102449763058784No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG14900.17503342081088638No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG14780.17362375567683896No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC14160.16634048581759403No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG13700.1609367694704123No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA13000.15271372285513576No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG12710.1493070321145212No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC12520.14707506231894613No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG12500.14684011812993822No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT12320.14472562042886714No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG12270.14413825995634738No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG12210.14343342738932366No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA11960.14049662502672491No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC11740.137912238947638No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11420.13415313192351158No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT11240.13203863422244047No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG10800.12686986206426665No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG10700.12569514111922714No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT10610.12463789226869158No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA10440.12264086666212441No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT10330.12134867362258096No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG10310.12111372943357307No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT10250.12040889686654936No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT10160.11935164801601379No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC9560.11230332234577675No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG9450.1110111293062333No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC9440.11089365721172935No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT9300.10924904788867404No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG9190.10795685484913059No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC9150.10748696647111479No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG9110.10701707809309899No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATA9020.10595982924256342No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC8810.10349291525798046No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG8680.10196577802942912No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG8630.10137841755690935No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCT8570.10067358498988566No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC8520.1000862245173659No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG2950.023.863429
TCTAGCG3100.022.7087228
TAGGACG957.2759576E-1121.8963854
TAGCGGC3250.021.66062430
CGTTTAT3050.021.50728831
ATCGTTT2950.021.1516529
TCGTTTA3050.020.45815330
GTCCTAG555.827637E-520.365171
TAACAGT555.827637E-520.365174
CGGCATC3200.019.49917825
CATCGTT3200.019.49917828
TATAAGA751.5073892E-619.2003172
GCATCGT3350.019.1036727
CGAGCCG3700.018.59381115
AAGACGG3450.018.0883185
GGCATCG3450.018.08619526
CAAGACG3500.017.8299124
TACTAAC450.008844607517.7791161
CGCAAGA3150.017.7780722
CGGCTAA450.00884760217.778079
Produced by FastQC (version 0.11.5)