##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062869_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	851266
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.144375553587246	32.0	32.0	32.0	32.0	32.0
2	31.264453179147292	32.0	32.0	32.0	32.0	32.0
3	31.371748666104367	32.0	32.0	32.0	32.0	32.0
4	31.481215037367875	32.0	32.0	32.0	32.0	32.0
5	31.38540009820667	32.0	32.0	32.0	32.0	32.0
6	34.934607983873434	36.0	36.0	36.0	36.0	36.0
7	34.95522786062171	36.0	36.0	36.0	36.0	36.0
8	34.89016476635975	36.0	36.0	36.0	36.0	36.0
9	35.00419845265757	36.0	36.0	36.0	36.0	36.0
10	34.8458683889642	36.0	36.0	36.0	32.0	36.0
11	35.03455911548212	36.0	36.0	36.0	36.0	36.0
12	34.91783179405732	36.0	36.0	36.0	32.0	36.0
13	34.96822379843668	36.0	36.0	36.0	36.0	36.0
14	34.910564970291304	36.0	36.0	36.0	32.0	36.0
15	34.87576151285262	36.0	36.0	36.0	32.0	36.0
16	34.886699339571884	36.0	36.0	36.0	32.0	36.0
17	34.838857654364205	36.0	36.0	36.0	32.0	36.0
18	34.8404928659197	36.0	36.0	36.0	32.0	36.0
19	34.831304198687604	36.0	36.0	36.0	32.0	36.0
20	34.812502789962245	36.0	36.0	36.0	32.0	36.0
21	34.80254702995303	36.0	36.0	36.0	32.0	36.0
22	34.775322872051746	36.0	36.0	36.0	32.0	36.0
23	34.702319838922264	36.0	36.0	36.0	32.0	36.0
24	34.680828319232766	36.0	36.0	36.0	32.0	36.0
25	34.64460814833436	36.0	36.0	36.0	32.0	36.0
26	34.57641324803293	36.0	36.0	36.0	32.0	36.0
27	34.56065436655523	36.0	36.0	36.0	32.0	36.0
28	34.51740114135887	36.0	36.0	36.0	32.0	36.0
29	34.47262900198058	36.0	36.0	36.0	32.0	36.0
30	34.44010685261716	36.0	36.0	36.0	32.0	36.0
31	34.44425714171599	36.0	36.0	36.0	32.0	36.0
32	34.41002929754037	36.0	36.0	36.0	32.0	36.0
33	34.34338737832828	36.0	36.0	36.0	32.0	36.0
34	34.33839481431186	36.0	36.0	36.0	32.0	36.0
35	34.28876520382583	36.0	36.0	36.0	32.0	36.0
36	34.23534711829205	36.0	36.0	36.0	32.0	36.0
37	34.23100182551635	36.0	36.0	36.0	32.0	36.0
38	33.754758207187884	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	26.0
22	67.0
23	209.0
24	622.0
25	1440.0
26	3014.0
27	6021.0
28	10418.0
29	16418.0
30	24848.0
31	35481.0
32	51485.0
33	82991.0
34	198119.0
35	420105.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.37621228907198	17.82574523102661	11.72091886665437	26.07712361324704
2	16.362922987644286	20.01959434536326	35.68414573118156	27.9333369358109
3	18.42784746483473	24.317898283262814	27.955304217483135	29.298950034419324
4	12.056424328997819	15.05854822945643	34.97563623035862	37.90939121118713
5	14.541400690266027	36.80800125930085	32.15446170762136	16.496136342811766
6	35.781731636719044	34.691000676640854	15.809079204571086	13.718188482069019
7	31.0400687920626	29.83256604026262	20.39985386385609	18.727511303818687
8	29.7791483113069	31.45221732745962	18.619324522760646	20.149309838472835
9	27.575654339551715	13.342770546496874	18.07727550397068	41.004299609980734
10	16.607949853217754	25.51943528483039	29.62381690221918	28.24879795973268
11	39.099226525567886	20.699721812354916	20.691263404227325	19.509788257849873
12	25.295383581630183	23.251721553544954	27.24683001552981	24.20606484929505
13	29.7174078015934	18.09830580949226	25.525631356738582	26.65865503217576
14	24.337868539328483	19.135499362126527	23.817584632770487	32.7090474657745
15	25.986824329880438	26.316568499153025	21.97409505371999	25.72251211724655
16	27.028214447657962	24.926403732793275	22.64803245988915	25.397349359659614
17	24.603825361285427	25.485688374726585	24.33481426487138	25.57567199911661
18	26.620703751823754	22.873813825525747	25.934666719920685	24.570815702729817
19	26.135426529427935	24.284536208423688	24.741502656044055	24.83853460610432
20	26.354981874055817	22.648619820361674	24.880237199653223	26.116161105929287
21	28.211309051822873	23.092339048357445	23.509482945968646	25.186868953851032
22	26.42992907034934	23.017012308726063	24.72282459301793	25.830234027906673
23	24.196663306948977	22.711456637322	25.632531465048363	27.45934859068066
24	25.0996750721866	24.462271487408167	24.730460279160685	25.707593161244546
25	25.533999400891616	23.391305880072597	24.66799410289393	26.40670061614186
26	25.186956838403034	24.485413490025447	25.30936276087615	25.018266910695363
27	26.31844805266509	23.581935611195558	24.469789701456417	25.629826634682928
28	24.972217849649816	23.43063155347447	25.610561211184287	25.986589385691428
29	24.6565703317177	23.891944468591486	25.88532844022902	25.56615675946179
30	24.834775499080195	24.102806878226076	26.0540183679367	25.00839925475703
31	25.493676477152853	24.152380102106743	24.046537744958684	26.30740567578172
32	25.185194756985478	23.885366031299267	24.067330305685886	26.862108906029373
33	24.333757016020844	23.483963884379268	25.13867580756191	27.043603292037975
34	25.781835524971044	23.82310582121217	25.187074310497543	25.207984343319247
35	26.89735053438056	23.377534166758686	25.20539995724016	24.5197153416206
36	24.786611940333575	24.903026785986988	24.411406070487956	25.89895520319148
37	26.564199674367355	24.293933975984004	24.562827600303546	24.57903874934509
38	25.107193286234853	23.57030587383967	25.02261337819201	26.29988746173347
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	24.0
1	39.0
2	54.0
3	54.0
4	264.5
5	475.0
6	475.0
7	706.0
8	937.0
9	963.0
10	989.0
11	989.0
12	1235.5
13	1482.0
14	1955.0
15	2428.0
16	2428.0
17	3509.5
18	4591.0
19	4591.0
20	5128.5
21	5666.0
22	5263.5
23	4861.0
24	4861.0
25	5163.0
26	5465.0
27	5465.0
28	6992.0
29	8519.0
30	10794.0
31	13069.0
32	13069.0
33	17810.5
34	22552.0
35	22552.0
36	25541.5
37	28531.0
38	34037.5
39	39544.0
40	39544.0
41	42387.5
42	45231.0
43	54584.5
44	63938.0
45	63938.0
46	67833.0
47	71728.0
48	71728.0
49	77594.0
50	83460.0
51	83450.0
52	83440.0
53	83440.0
54	78622.5
55	73805.0
56	73805.0
57	72951.5
58	72098.0
59	65044.5
60	57991.0
61	57991.0
62	56865.5
63	55740.0
64	46723.0
65	37706.0
66	37706.0
67	32197.0
68	26688.0
69	26688.0
70	21402.0
71	16116.0
72	12645.0
73	9174.0
74	9174.0
75	6790.5
76	4407.0
77	4407.0
78	4431.0
79	4455.0
80	3507.0
81	2559.0
82	2559.0
83	2422.5
84	2286.0
85	2286.0
86	1462.5
87	639.0
88	540.0
89	441.0
90	441.0
91	269.0
92	97.0
93	71.5
94	46.0
95	46.0
96	33.5
97	21.0
98	21.0
99	17.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010220072221843702
2	0.0
3	0.0
4	2.3494418900790116E-4
5	0.0
6	2.3494418900790116E-4
7	5.873604725197529E-4
8	0.0018795535120632093
9	0.0030542744571027155
10	5.873604725197529E-4
11	0.004933827969165925
12	0.0
13	4.698883780158023E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.1747209450395058E-4
22	0.0
23	4.698883780158023E-4
24	0.0
25	1.1747209450395058E-4
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	851266.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.19976571298443
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.84988165574421	26.682491143291177
2	15.515946523064306	11.854110439925037
3	5.931559867425378	6.7975259174457126
4	2.8286789075580243	4.32219486183909
5	1.5705688193105578	2.999768046689094
6	0.9431106397070177	2.161596328693857
7	0.6173431834316979	1.650765548011918
8	0.4394461755685029	1.3429392760147068
9	0.31485493089021016	1.082464613422554
>10	1.6355514702176457	11.27033049268716
>50	0.15294038250856348	4.119282806323738
>100	0.16650758100141752	13.868827183807438
>500	0.023126052916840792	6.00294629531212
>1k	0.01048381065563449	5.844757046536431
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3630	0.42642370304934063	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2581	0.3031954759146965	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2123	0.2493932566318871	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1974	0.23188991455079846	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1834	0.21544382132024537	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1817	0.21344679571367822	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1698	0.19946761646770808	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1669	0.19606092572709352	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1666	0.19570850944358167	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1585	0.1861932697887617	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1541	0.18102449763058784	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1490	0.17503342081088638	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1478	0.17362375567683896	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1416	0.16634048581759403	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1370	0.1609367694704123	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1300	0.15271372285513576	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1271	0.1493070321145212	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1252	0.14707506231894613	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1250	0.14684011812993822	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1232	0.14472562042886714	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1227	0.14413825995634738	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1221	0.14343342738932366	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1196	0.14049662502672491	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1174	0.137912238947638	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1142	0.13415313192351158	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1124	0.13203863422244047	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1080	0.12686986206426665	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	1070	0.12569514111922714	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1061	0.12463789226869158	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1044	0.12264086666212441	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT	1033	0.12134867362258096	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	1031	0.12111372943357307	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1025	0.12040889686654936	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1016	0.11935164801601379	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	956	0.11230332234577675	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	945	0.1110111293062333	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	944	0.11089365721172935	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT	930	0.10924904788867404	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG	919	0.10795685484913059	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC	915	0.10748696647111479	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG	911	0.10701707809309899	No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATA	902	0.10595982924256342	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC	881	0.10349291525798046	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG	868	0.10196577802942912	No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG	863	0.10137841755690935	No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCT	857	0.10067358498988566	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	852	0.1000862245173659	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.1747209450395058E-4
7	0.0	0.0	0.0	0.0	1.1747209450395058E-4
8	0.0	0.0	0.0	0.0	1.1747209450395058E-4
9	0.0	0.0	0.0	0.0	1.1747209450395058E-4
10	0.0	0.0	0.0	0.0	1.1747209450395058E-4
11	0.0	0.0	0.0	1.1747209450395058E-4	1.1747209450395058E-4
12	0.0	0.0	0.0	1.1747209450395058E-4	5.873604725197529E-4
13	0.0	0.0	0.0	2.3494418900790116E-4	7.048325670237036E-4
14	0.0	0.0	0.0	2.3494418900790116E-4	7.048325670237036E-4
15	0.0	0.0	0.0	2.3494418900790116E-4	7.048325670237036E-4
16	0.0	0.0	0.0	2.3494418900790116E-4	7.048325670237036E-4
17	0.0	0.0	0.0	2.3494418900790116E-4	7.048325670237036E-4
18	0.0	0.0	0.0	2.3494418900790116E-4	7.048325670237036E-4
19	0.0	0.0	0.0	4.698883780158023E-4	7.048325670237036E-4
20	0.0	0.0	1.1747209450395058E-4	4.698883780158023E-4	7.048325670237036E-4
21	0.0	0.0	1.1747209450395058E-4	7.048325670237036E-4	7.048325670237036E-4
22	0.0	0.0	1.1747209450395058E-4	0.0011747209450395058	8.223046615276541E-4
23	0.0	0.0	1.1747209450395058E-4	0.0018795535120632093	8.223046615276541E-4
24	0.0	0.0	1.1747209450395058E-4	0.002584386079086913	8.223046615276541E-4
25	1.1747209450395058E-4	0.0	1.1747209450395058E-4	0.0035241628351185177	8.223046615276541E-4
26	1.1747209450395058E-4	0.0	1.1747209450395058E-4	0.004346467496646172	9.397767560316046E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	295	0.0	23.8634	29
TCTAGCG	310	0.0	22.70872	28
TAGGACG	95	7.2759576E-11	21.896385	4
TAGCGGC	325	0.0	21.660624	30
CGTTTAT	305	0.0	21.507288	31
ATCGTTT	295	0.0	21.15165	29
TCGTTTA	305	0.0	20.458153	30
GTCCTAG	55	5.827637E-5	20.36517	1
TAACAGT	55	5.827637E-5	20.36517	4
CGGCATC	320	0.0	19.499178	25
CATCGTT	320	0.0	19.499178	28
TATAAGA	75	1.5073892E-6	19.200317	2
GCATCGT	335	0.0	19.10367	27
CGAGCCG	370	0.0	18.593811	15
AAGACGG	345	0.0	18.088318	5
GGCATCG	345	0.0	18.086195	26
CAAGACG	350	0.0	17.829912	4
TACTAAC	45	0.0088446075	17.779116	1
CGCAAGA	315	0.0	17.778072	2
CGGCTAA	45	0.008847602	17.77807	9
>>END_MODULE