FastQCFastQC Report
Thu 2 Feb 2017
SRR4062868_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062868_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1129112
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT23630.20927950460184638No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT15380.1362132365965467No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT15310.13559328038316837No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC13910.12319415611560235No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG12880.11407194326160736No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA12370.10955511942127973No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC12330.10920085872792071No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG12110.10725242491444603No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC11800.10450690454091358No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG11710.10370981798085575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTTA404.9603495E-628.00023317
TCTAGCG2650.021.73506728
GATTAGG453.5164578E-421.351481
TCGACGC707.7834557E-720.571614
CTAGCGG2800.019.9992829
CGAACGA1300.019.6924716
GCGTAAC1257.2759576E-1219.2001611
ACGAACG1351.8189894E-1218.9631215
GTTCTAG852.3745815E-718.839541
TAGCGGC3000.018.66599730
ATCGTTT2850.018.52564829
CATCGTT2850.018.52564828
CGCGTAA1301.2732926E-1118.4616910
TCGCGTA1352.3646862E-1117.7779259
AACCGGT450.00885201217.777147
TCGTTTA2900.017.65453730
GCATCGT3000.017.59936727
TAGGACG2200.017.4515974
GTCTTAG1404.0017767E-1117.1574381
GTAGGAC2800.017.1433
Produced by FastQC (version 0.11.5)