##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062868_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1129112
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.74248967330079	32.0	32.0	32.0	32.0	32.0
2	30.08336019810258	32.0	32.0	32.0	21.0	32.0
3	30.16612878084725	32.0	32.0	32.0	21.0	32.0
4	30.03018566802939	32.0	32.0	32.0	21.0	32.0
5	30.043574950934893	32.0	32.0	32.0	21.0	32.0
6	33.39332856262266	36.0	36.0	36.0	21.0	36.0
7	33.40108952876243	36.0	36.0	36.0	21.0	36.0
8	33.29522314881075	36.0	36.0	36.0	21.0	36.0
9	33.57235066140471	36.0	36.0	36.0	21.0	36.0
10	32.871599982995484	36.0	32.0	36.0	21.0	36.0
11	33.713075407931186	36.0	36.0	36.0	27.0	36.0
12	33.226730386356714	36.0	32.0	36.0	21.0	36.0
13	33.470484770332796	36.0	36.0	36.0	21.0	36.0
14	33.159537760647304	36.0	32.0	36.0	21.0	36.0
15	33.055370060720286	36.0	32.0	36.0	21.0	36.0
16	33.13090109749963	36.0	32.0	36.0	21.0	36.0
17	32.97418768023013	36.0	32.0	36.0	21.0	36.0
18	33.04904916429903	36.0	32.0	36.0	21.0	36.0
19	32.98527249732533	36.0	32.0	36.0	21.0	36.0
20	32.95588834411467	36.0	32.0	36.0	21.0	36.0
21	32.9389042008233	36.0	32.0	36.0	21.0	36.0
22	32.886464761688835	36.0	32.0	36.0	21.0	36.0
23	32.84259223177152	36.0	32.0	36.0	21.0	36.0
24	32.82328502398345	36.0	32.0	36.0	21.0	36.0
25	32.820141845981624	36.0	32.0	36.0	21.0	36.0
26	32.77809818689377	36.0	32.0	36.0	21.0	36.0
27	32.76997853180198	36.0	32.0	36.0	21.0	36.0
28	32.72948564889931	36.0	32.0	36.0	21.0	36.0
29	32.692410496035826	36.0	32.0	36.0	21.0	36.0
30	32.65509090329392	36.0	32.0	36.0	21.0	36.0
31	32.67886533842524	36.0	32.0	36.0	21.0	36.0
32	32.64193897505296	36.0	32.0	36.0	21.0	36.0
33	32.57110897767449	36.0	32.0	36.0	14.0	36.0
34	32.59935418275601	36.0	32.0	36.0	14.0	36.0
35	32.5932865827305	36.0	32.0	36.0	14.0	36.0
36	32.5349247904548	36.0	32.0	36.0	14.0	36.0
37	32.585671749126746	36.0	32.0	36.0	14.0	36.0
38	31.543280914559407	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	5.0
10	16.0
11	9.0
12	6.0
13	6.0
14	306.0
15	669.0
16	931.0
17	1268.0
18	1984.0
19	2755.0
20	3710.0
21	4987.0
22	6274.0
23	8166.0
24	11210.0
25	14893.0
26	20354.0
27	27350.0
28	37048.0
29	49672.0
30	65951.0
31	87387.0
32	117764.0
33	165725.0
34	259428.0
35	241237.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.432039213738996	17.078867284760697	11.178540267147143	26.31055323435316
2	16.712390917533302	19.372372627832952	37.47642897145247	26.438807483181275
3	19.19230748790516	23.081876431584224	28.466847476603064	29.258968603907547
4	12.509122471392638	14.782654904878308	35.90976724412796	36.798455379601094
5	14.568124069509675	36.422435743581886	33.92533404658009	15.084106140328347
6	34.969077387568426	35.30110910461258	16.37583262554811	13.35398088227089
7	30.586779699445227	30.181594031415838	20.67660249824641	18.55502377089252
8	28.249392663785933	33.21958602830014	19.265422088705183	19.265599219208738
9	27.270746631642062	13.987021648004491	18.418561151206568	40.32367056914688
10	15.53993853566083	26.676585983650842	31.945337478190787	25.83813800249754
11	38.094769956744	20.864110276221957	21.870848433962866	19.170271333071177
12	24.434320210010945	23.514636643509252	29.026538326643546	23.024504819836256
13	29.569219567545446	19.099943221627864	25.330771663251994	26.000065547574696
14	23.237015825383146	19.564768748521935	25.015743895752824	32.18247153034209
15	25.048145320026862	27.363372705899863	22.193796650467633	25.39468532360565
16	25.815486244772977	25.69706170742142	23.8143152856228	24.6731367621828
17	23.970176462720136	25.97778204936279	25.321217779675386	24.73082370824169
18	24.917806569097394	24.799476371160026	26.276655294213512	24.006061765529065
19	25.48331524988375	25.05994109962135	25.32167135360156	24.135072296893338
20	25.501529609953888	24.35489785181472	25.130331270825103	25.01324126740629
21	26.45948825686887	24.29190030849135	24.548666436973615	24.699944997666165
22	25.498968140793426	24.426188852377706	25.085515885317484	24.989327121511387
23	24.128881283392147	24.156516162861557	25.736398679194476	25.97820387455182
24	24.646110156933016	25.117697548082162	25.22850818413167	25.007684110853152
25	24.780987306564622	24.659987776035717	25.342935345858468	25.216089571541193
26	24.39771923002311	25.421443737670707	25.757252304642414	24.42358472766377
27	25.177655802566672	24.873938117410933	24.97314016097303	24.97526591904936
28	24.337475742945635	24.771822049097782	25.703487790210026	25.187214417746556
29	24.2919372610709	25.13560721207161	25.617894128942886	24.954561397914606
30	24.288090438553294	25.213271737243552	25.904013547360847	24.594624276842303
31	24.934480373340087	24.96760553774807	24.83979908790657	25.258115001005272
32	24.515801603321787	25.105245117273128	24.724476121559107	25.654477157845978
33	24.191044067538396	24.661364709300216	25.465427004199228	25.68216421896216
34	24.936756624610705	24.872538911505334	25.50887001626258	24.681834447621377
35	25.43288547800761	24.82045040640036	25.50268571469065	24.24397840090138
36	24.266066463469187	25.529717410233037	25.136343384256715	25.067872742041065
37	25.327809900372717	25.093533438204158	24.88689185550501	24.691764805918115
38	24.314445165798826	25.042137232307898	25.559120994748678	25.084296607144594
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	64.0
1	88.0
2	112.0
3	112.0
4	362.0
5	612.0
6	612.0
7	725.0
8	838.0
9	791.5
10	745.0
11	745.0
12	949.0
13	1153.0
14	1649.0
15	2145.0
16	2145.0
17	3235.5
18	4326.0
19	4326.0
20	5450.0
21	6574.0
22	7276.0
23	7978.0
24	7978.0
25	9698.0
26	11418.0
27	11418.0
28	14450.5
29	17483.0
30	21563.0
31	25643.0
32	25643.0
33	32374.0
34	39105.0
35	39105.0
36	44347.5
37	49590.0
38	56156.5
39	62723.0
40	62723.0
41	67096.0
42	71469.0
43	80346.0
44	89223.0
45	89223.0
46	92549.0
47	95875.0
48	95875.0
49	100636.5
50	105398.0
51	106701.0
52	108004.0
53	108004.0
54	102063.0
55	96122.0
56	96122.0
57	92922.0
58	89722.0
59	80363.0
60	71004.0
61	71004.0
62	66243.0
63	61482.0
64	51076.5
65	40671.0
66	40671.0
67	33833.5
68	26996.0
69	26996.0
70	21691.5
71	16387.0
72	12933.5
73	9480.0
74	9480.0
75	7285.5
76	5091.0
77	5091.0
78	4884.5
79	4678.0
80	3632.0
81	2586.0
82	2586.0
83	2537.0
84	2488.0
85	2488.0
86	1596.0
87	704.0
88	576.5
89	449.0
90	449.0
91	312.0
92	175.0
93	136.5
94	98.0
95	98.0
96	85.0
97	72.0
98	72.0
99	250.5
100	429.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11903159296863376
2	0.05384762539057241
3	0.010450690454091357
4	0.002834085546872232
5	2.6569552001927177E-4
6	2.6569552001927177E-4
7	0.0
8	8.856517333975725E-5
9	8.856517333975725E-5
10	1.771303466795145E-4
11	0.0017713034667951454
12	0.002479824853513203
13	0.01425899290770092
14	0.009122212853994997
15	0.02107851125486223
16	0.010893516320790143
17	0.018155860534650237
18	0.0060224317871034935
19	0.0077051700805588815
20	0.005845301440423979
21	0.0061109969604432515
22	0.007262344213860096
23	0.009210778027334755
24	0.014081862561021403
25	0.01611886154783582
26	0.017270208801252667
27	0.009122212853994997
28	0.005225345227045678
29	0.010273560107411843
30	0.0031883462402312614
31	0.005402475573725193
32	0.007173779040520339
33	0.008413691467276939
34	0.012399124267566017
35	0.015587470507797277
36	0.01425899290770092
37	0.009210778027334755
38	0.005579605920404707
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1129112.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.881324831909126
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.83741325397696	50.432514645504654
2	10.918332063511786	13.294450419626191
3	2.919860965848235	5.33295011777455
4	1.1235965665630452	2.7362419019577024
5	0.6011022938644897	1.8297952004984845
6	0.355603098141878	1.298975263752536
7	0.21881798465026156	0.9325350161789395
8	0.15567299888773095	0.7582062730273101
9	0.11376315704486736	0.6233446546156854
>10	0.6176863119604564	6.951873944825304
>50	0.06085050996377597	2.616908168432032
>100	0.0685497652078236	8.7452259145981
>500	0.0065632727840504905	2.7271829642366177
>1k	0.002187757594683497	1.7197955149719928
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2363	0.20927950460184638	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1538	0.1362132365965467	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1531	0.13559328038316837	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1391	0.12319415611560235	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1288	0.11407194326160736	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1237	0.10955511942127973	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1233	0.10920085872792071	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1211	0.10725242491444603	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1180	0.10450690454091358	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1171	0.10370981798085575	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.856517333975726E-5
2	0.0	0.0	0.0	8.856517333975726E-5	8.856517333975726E-5
3	0.0	0.0	0.0	8.856517333975726E-5	8.856517333975726E-5
4	0.0	0.0	0.0	8.856517333975726E-5	8.856517333975726E-5
5	0.0	0.0	0.0	8.856517333975726E-5	8.856517333975726E-5
6	0.0	0.0	0.0	8.856517333975726E-5	8.856517333975726E-5
7	0.0	0.0	0.0	1.7713034667951453E-4	8.856517333975726E-5
8	0.0	0.0	0.0	1.7713034667951453E-4	8.856517333975726E-5
9	0.0	0.0	0.0	1.7713034667951453E-4	8.856517333975726E-5
10	8.856517333975726E-5	0.0	0.0	1.7713034667951453E-4	8.856517333975726E-5
11	8.856517333975726E-5	0.0	0.0	1.7713034667951453E-4	8.856517333975726E-5
12	8.856517333975726E-5	0.0	0.0	1.7713034667951453E-4	2.6569552001927177E-4
13	8.856517333975726E-5	0.0	0.0	1.7713034667951453E-4	2.6569552001927177E-4
14	8.856517333975726E-5	0.0	0.0	1.7713034667951453E-4	2.6569552001927177E-4
15	8.856517333975726E-5	0.0	0.0	2.6569552001927177E-4	2.6569552001927177E-4
16	8.856517333975726E-5	0.0	0.0	2.6569552001927177E-4	2.6569552001927177E-4
17	1.7713034667951453E-4	0.0	0.0	2.6569552001927177E-4	2.6569552001927177E-4
18	1.7713034667951453E-4	0.0	0.0	3.5426069335902906E-4	2.6569552001927177E-4
19	1.7713034667951453E-4	0.0	0.0	3.5426069335902906E-4	2.6569552001927177E-4
20	1.7713034667951453E-4	0.0	0.0	4.428258666987863E-4	3.5426069335902906E-4
21	1.7713034667951453E-4	0.0	0.0	5.313910400385435E-4	3.5426069335902906E-4
22	1.7713034667951453E-4	0.0	0.0	6.199562133783008E-4	3.5426069335902906E-4
23	1.7713034667951453E-4	0.0	0.0	7.970865600578154E-4	4.428258666987863E-4
24	1.7713034667951453E-4	0.0	0.0	8.856517333975726E-4	4.428258666987863E-4
25	1.7713034667951453E-4	0.0	0.0	0.0011513472534168444	4.428258666987863E-4
26	1.7713034667951453E-4	0.0	0.0	0.0015056079467758734	4.428258666987863E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCTTA	40	4.9603495E-6	28.000233	17
TCTAGCG	265	0.0	21.735067	28
GATTAGG	45	3.5164578E-4	21.35148	1
TCGACGC	70	7.7834557E-7	20.5716	14
CTAGCGG	280	0.0	19.99928	29
CGAACGA	130	0.0	19.69247	16
GCGTAAC	125	7.2759576E-12	19.20016	11
ACGAACG	135	1.8189894E-12	18.96312	15
GTTCTAG	85	2.3745815E-7	18.83954	1
TAGCGGC	300	0.0	18.665997	30
ATCGTTT	285	0.0	18.525648	29
CATCGTT	285	0.0	18.525648	28
CGCGTAA	130	1.2732926E-11	18.46169	10
TCGCGTA	135	2.3646862E-11	17.777925	9
AACCGGT	45	0.008852012	17.77714	7
TCGTTTA	290	0.0	17.654537	30
GCATCGT	300	0.0	17.599367	27
TAGGACG	220	0.0	17.451597	4
GTCTTAG	140	4.0017767E-11	17.157438	1
GTAGGAC	280	0.0	17.143	3
>>END_MODULE