FastQCFastQC Report
Thu 2 Feb 2017
SRR4062868_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062868_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1129112
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT23830.21105080806864154No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT18310.16216283238509555No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC16230.14374127633042605No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT14480.12824237099596852No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG12790.11327485670154953No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC12400.109820814941299No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG11690.10353268763417624No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC11670.10335555728749674No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG11360.10061003691396425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG2200.018.1809371
GTTTTCG2650.018.1123328
CTAGCGG2750.018.0354929
AACCGCT450.00884136517.7808537
TAGTACG652.10114E-417.2299352
AAGACGG2950.016.816475
TCTAGCG2950.016.81274628
CAAGACG3250.016.7391344
ATACCGT2700.016.5954636
ACCGTCG2800.016.5742958
TAGCGGC2900.016.55092230
TTTTCGG2900.016.55092229
TAGGACG1850.016.4330924
TACCGTC2650.016.3047087
TTAGTAC600.00244193815.9999323
GGTTAGG908.584479E-615.9992261
GTATTAA1001.314982E-615.9992251
TATTAGC2000.015.9992252
ATCGTTT2550.015.68551529
GAACCGC2700.015.4100726
Produced by FastQC (version 0.11.5)