##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062868_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1129112
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.9490156866635	32.0	32.0	32.0	32.0	32.0
2	30.676865536811228	32.0	32.0	32.0	32.0	32.0
3	31.039933151007162	32.0	32.0	32.0	32.0	32.0
4	31.248184413946536	32.0	32.0	32.0	32.0	32.0
5	31.022794018662452	32.0	32.0	32.0	32.0	32.0
6	34.61915824116651	36.0	36.0	36.0	32.0	36.0
7	34.66508016919491	36.0	36.0	36.0	32.0	36.0
8	34.56825186518255	36.0	36.0	36.0	32.0	36.0
9	34.787844784219814	36.0	36.0	36.0	32.0	36.0
10	34.479585727545185	36.0	36.0	36.0	32.0	36.0
11	34.82655396453142	36.0	36.0	36.0	32.0	36.0
12	34.647817045607525	36.0	36.0	36.0	32.0	36.0
13	34.76070930076024	36.0	36.0	36.0	32.0	36.0
14	34.6796916514925	36.0	36.0	36.0	32.0	36.0
15	34.62094814331971	36.0	36.0	36.0	32.0	36.0
16	34.649034816740944	36.0	36.0	36.0	32.0	36.0
17	34.60537750019484	36.0	36.0	36.0	32.0	36.0
18	34.61524188920143	36.0	36.0	36.0	32.0	36.0
19	34.61546773039344	36.0	36.0	36.0	32.0	36.0
20	34.609788045827166	36.0	36.0	36.0	32.0	36.0
21	34.599291301482936	36.0	36.0	36.0	32.0	36.0
22	34.57845103054436	36.0	36.0	36.0	32.0	36.0
23	34.50403325799389	36.0	36.0	36.0	32.0	36.0
24	34.4862688555254	36.0	36.0	36.0	32.0	36.0
25	34.46567125316178	36.0	36.0	36.0	32.0	36.0
26	34.38718833915502	36.0	36.0	36.0	32.0	36.0
27	34.372524603405154	36.0	36.0	36.0	32.0	36.0
28	34.34217066154642	36.0	36.0	36.0	32.0	36.0
29	34.29202063214278	36.0	36.0	36.0	32.0	36.0
30	34.26916550351072	36.0	36.0	36.0	32.0	36.0
31	34.27561127682639	36.0	36.0	36.0	32.0	36.0
32	34.231560730910665	36.0	36.0	36.0	32.0	36.0
33	34.194337674207695	36.0	36.0	36.0	32.0	36.0
34	34.19390370485833	36.0	36.0	36.0	32.0	36.0
35	34.16015948816415	36.0	36.0	36.0	32.0	36.0
36	34.11838329589979	36.0	36.0	36.0	32.0	36.0
37	34.12169651903443	36.0	36.0	36.0	32.0	36.0
38	33.577669000063764	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	2.0
21	17.0
22	58.0
23	240.0
24	611.0
25	1680.0
26	3667.0
27	7622.0
28	13963.0
29	23287.0
30	37332.0
31	58271.0
32	89403.0
33	148679.0
34	321223.0
35	423056.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.32326372558658	18.709417992792112	12.22754532386335	26.739772957757957
2	15.787804929891808	20.57802946031926	36.412065410694424	27.222100199094513
3	18.207494030707316	24.144726121057964	28.89341358518907	28.75436626304565
4	12.260364357768507	15.903587781527042	36.215957701198285	35.620090159506155
5	14.573133577537037	36.61833369940272	33.12585465392273	15.682678069137518
6	35.091917609371635	35.99803030531154	15.809545402613919	13.100506682702909
7	30.438479258387378	30.359124511671613	20.49442612613331	18.7079701038077
8	28.250690156949265	33.54176058439931	18.751721517574758	19.45582774107667
9	27.068706684675508	13.696521488829353	17.987113344994796	41.247658481500345
10	15.246111729597263	26.949227748447658	31.88510150996235	25.919559011992728
11	38.45089658177644	20.74174583239598	21.588286247216725	19.219071338610853
12	24.35615276088888	23.555434319566455	28.794246092722503	23.294166826822163
13	29.65057372722539	19.07000924623685	25.20910311502531	26.07031391151245
14	23.236667398805434	19.40781782498105	24.926490906128002	32.429023870085516
15	25.092816301660065	27.495412324021	21.92067748814998	25.491093886168954
16	25.850048533714993	25.804348904271674	23.561081628748965	24.78452093326437
17	24.170764282019853	26.043652002635696	24.95961428095707	24.82596943438738
18	25.19980303105449	24.746969299768313	25.971825647057155	24.081402022120038
19	25.609771218444227	25.04056284938961	25.17155074075911	24.178115191407052
20	25.734913808373307	24.203710526502242	24.972367665917997	25.08900799920646
21	26.682342730114872	24.318445501324053	24.30941183764204	24.689799930919044
22	25.50738987806347	24.530958841992646	24.969710710717802	24.99194056922608
23	24.23871523703161	24.077880739803263	25.623344383324582	26.06005963984055
24	24.768756332409893	25.049685062243604	25.141084321130236	25.04047428421627
25	24.867948858745134	24.56868607785969	25.228941784134022	25.334423279261152
26	24.66389516717562	25.32308575234343	25.546624249852982	24.466394830627962
27	25.4094367963497	24.696575716137993	24.83544590793473	25.058541579577582
28	24.468343264441437	24.662300994055506	25.756523710668205	25.112832030834852
29	24.596585635437407	24.83615442932145	25.50331588008984	25.063944055151303
30	24.490927383643076	24.97077349279788	25.845797405394684	24.692501718164365
31	25.10317842694082	24.793731711291706	24.85138763913589	25.25170222263159
32	24.823135348840506	24.840316992468416	24.707292102112103	25.629255556578972
33	24.369327400647588	24.552568744287544	25.426087048937575	25.652016806127293
34	25.100167211047264	24.695247238537895	25.614376607457896	24.59020894295694
35	25.83215836870036	24.487384776709483	25.26259573895238	24.417861115637777
36	24.568599040662043	25.234254883483658	25.07297770283196	25.124168373022336
37	25.56185745966742	24.869720630017216	24.93463890207526	24.633783008240105
38	24.795945117738956	24.535739716661293	25.373657790043115	25.294657375556636
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	17.0
1	30.0
2	43.0
3	43.0
4	193.0
5	343.0
6	343.0
7	507.0
8	671.0
9	700.5
10	730.0
11	730.0
12	927.5
13	1125.0
14	1665.0
15	2205.0
16	2205.0
17	3388.5
18	4572.0
19	4572.0
20	5477.0
21	6382.0
22	7204.0
23	8026.0
24	8026.0
25	9790.5
26	11555.0
27	11555.0
28	14722.5
29	17890.0
30	21730.0
31	25570.0
32	25570.0
33	32686.0
34	39802.0
35	39802.0
36	44303.0
37	48804.0
38	55678.0
39	62552.0
40	62552.0
41	66581.5
42	70611.0
43	79263.0
44	87915.0
45	87915.0
46	91360.5
47	94806.0
48	94806.0
49	100015.5
50	105225.0
51	106363.5
52	107502.0
53	107502.0
54	101693.5
55	95885.0
56	95885.0
57	92696.5
58	89508.0
59	80593.5
60	71679.0
61	71679.0
62	67066.0
63	62453.0
64	51991.5
65	41530.0
66	41530.0
67	34912.0
68	28294.0
69	28294.0
70	22763.0
71	17232.0
72	13602.5
73	9973.0
74	9973.0
75	7637.0
76	5301.0
77	5301.0
78	4970.5
79	4640.0
80	3687.5
81	2735.0
82	2735.0
83	2455.5
84	2176.0
85	2176.0
86	1463.0
87	750.0
88	592.0
89	434.0
90	434.0
91	267.5
92	101.0
93	74.0
94	47.0
95	47.0
96	29.0
97	11.0
98	11.0
99	14.0
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.006288127307122766
2	0.0
3	0.0
4	1.771303466795145E-4
5	0.0
6	2.6569552001927177E-4
7	4.4282586669878635E-4
8	0.0018598686401349025
9	0.0032769114135710186
10	9.742169067373298E-4
11	0.004073997973628834
12	8.856517333975725E-5
13	3.54260693359029E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.771303466795145E-4
22	0.0
23	8.856517333975725E-5
24	0.0
25	3.54260693359029E-4
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	3.54260693359029E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1129112.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.32153642767143
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.37923133610624	49.692418039245055
2	11.205041615265422	13.518106519376152
3	3.008894681561143	5.44503450422452
4	1.1421317328857612	2.755805637218718
5	0.613142833918214	1.8492858895781623
6	0.35976554687798556	1.3020966324852925
7	0.22731255433358585	0.959828976869031
8	0.17454936374779229	0.8423268642991459
9	0.12259232873932704	0.665546186142215
>10	0.6232279582566863	6.867850311609754
>50	0.06550990004404676	2.7542389747093
>100	0.06871983262323934	8.51393536541336
>500	0.007668304676281927	3.0967764889217984
>1k	0.002212010964312094	1.7367496099074824
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2383	0.21105080806864154	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1831	0.16216283238509555	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1623	0.14374127633042605	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1448	0.12824237099596852	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1279	0.11327485670154953	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1240	0.109820814941299	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1169	0.10353268763417624	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1167	0.10335555728749674	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1136	0.10061003691396425	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	8.856517333975726E-5	0.0	0.0	0.0	0.0
12	8.856517333975726E-5	0.0	0.0	0.0	8.856517333975726E-5
13	8.856517333975726E-5	0.0	0.0	0.0	2.6569552001927177E-4
14	8.856517333975726E-5	0.0	0.0	0.0	2.6569552001927177E-4
15	8.856517333975726E-5	0.0	0.0	8.856517333975726E-5	3.5426069335902906E-4
16	8.856517333975726E-5	0.0	0.0	8.856517333975726E-5	3.5426069335902906E-4
17	8.856517333975726E-5	0.0	0.0	8.856517333975726E-5	3.5426069335902906E-4
18	8.856517333975726E-5	0.0	0.0	1.7713034667951453E-4	3.5426069335902906E-4
19	8.856517333975726E-5	0.0	0.0	1.7713034667951453E-4	3.5426069335902906E-4
20	8.856517333975726E-5	0.0	0.0	2.6569552001927177E-4	3.5426069335902906E-4
21	8.856517333975726E-5	0.0	0.0	3.5426069335902906E-4	3.5426069335902906E-4
22	8.856517333975726E-5	0.0	0.0	4.428258666987863E-4	3.5426069335902906E-4
23	8.856517333975726E-5	0.0	0.0	4.428258666987863E-4	3.5426069335902906E-4
24	8.856517333975726E-5	0.0	0.0	5.313910400385435E-4	3.5426069335902906E-4
25	8.856517333975726E-5	0.0	0.0	7.970865600578154E-4	3.5426069335902906E-4
26	8.856517333975726E-5	0.0	0.0	8.856517333975726E-4	3.5426069335902906E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAG	220	0.0	18.180937	1
GTTTTCG	265	0.0	18.11233	28
CTAGCGG	275	0.0	18.03549	29
AACCGCT	45	0.008841365	17.780853	7
TAGTACG	65	2.10114E-4	17.229935	2
AAGACGG	295	0.0	16.81647	5
TCTAGCG	295	0.0	16.812746	28
CAAGACG	325	0.0	16.739134	4
ATACCGT	270	0.0	16.595463	6
ACCGTCG	280	0.0	16.574295	8
TAGCGGC	290	0.0	16.550922	30
TTTTCGG	290	0.0	16.550922	29
TAGGACG	185	0.0	16.433092	4
TACCGTC	265	0.0	16.304708	7
TTAGTAC	60	0.002441938	15.999932	3
GGTTAGG	90	8.584479E-6	15.999226	1
GTATTAA	100	1.314982E-6	15.999225	1
TATTAGC	200	0.0	15.999225	2
ATCGTTT	255	0.0	15.685515	29
GAACCGC	270	0.0	15.410072	6
>>END_MODULE