FastQCFastQC Report
Thu 2 Feb 2017
SRR4062867_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062867_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1445988
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT73680.5095477970771541No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT54140.3744152786883432No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT30790.21293399391972825No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA30650.2119657977797879No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC23390.16175791223716934No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21460.14841063687942085No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA19170.13257371430468304No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA16360.11314063463873836No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG16330.11293316403732256No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG15490.10712398719768075No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC14740.10193722216228628No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG14470.10006998674954426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG3750.018.34601228
AGTGTCG450.00885140217.7777610
CTAGCGG3800.017.6835829
TAGATCG550.001366785317.4509055
CATCGTT3350.017.19341728
TAGCGGC4000.016.799430
CAAGACG3900.016.4056994
CGAGCCG3700.016.0010915
AAGACGG4100.015.9966635
CGGCATC3450.015.76755225
TCGTTTA3800.015.57839230
CGTTTAT3950.015.39185531
AGGCCCG3750.015.359982510
GGCATCG3550.015.32339726
ATCGTTT3900.015.178946529
GTATTAG2850.015.1715221
AGCGGCG4800.014.99946531
GCATCGT3850.014.95998827
ACCGTCG3000.014.9322848
TTCGCGT650.004163256414.76870431
Produced by FastQC (version 0.11.5)