##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062867_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1445988
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.09778366072194	32.0	32.0	32.0	32.0	32.0
2	30.685587985515784	32.0	32.0	32.0	32.0	32.0
3	30.759908795923618	32.0	32.0	32.0	32.0	32.0
4	30.807669911506874	32.0	32.0	32.0	32.0	32.0
5	30.73846325142394	32.0	32.0	32.0	32.0	32.0
6	34.36273330069129	36.0	36.0	36.0	32.0	36.0
7	34.254937108745025	36.0	36.0	36.0	32.0	36.0
8	34.22264638434067	36.0	36.0	36.0	32.0	36.0
9	34.36789655239186	36.0	36.0	36.0	32.0	36.0
10	34.077137569606386	36.0	36.0	36.0	32.0	36.0
11	34.41886931288503	36.0	36.0	36.0	32.0	36.0
12	34.20449270671679	36.0	36.0	36.0	32.0	36.0
13	34.29916638312351	36.0	36.0	36.0	32.0	36.0
14	34.192144056520526	36.0	36.0	36.0	32.0	36.0
15	34.14124045289449	36.0	36.0	36.0	32.0	36.0
16	34.15197705651776	36.0	36.0	36.0	32.0	36.0
17	34.073623709187075	36.0	36.0	36.0	32.0	36.0
18	34.10244552513576	36.0	36.0	36.0	32.0	36.0
19	34.08971443746421	36.0	36.0	36.0	32.0	36.0
20	34.06042788736836	36.0	36.0	36.0	32.0	36.0
21	34.03622851641922	36.0	36.0	36.0	32.0	36.0
22	34.013363873005865	36.0	36.0	36.0	32.0	36.0
23	33.96307507392869	36.0	36.0	36.0	32.0	36.0
24	33.95717391845575	36.0	36.0	36.0	32.0	36.0
25	33.922046379361376	36.0	36.0	36.0	32.0	36.0
26	33.88627153199058	36.0	36.0	36.0	32.0	36.0
27	33.88664221279845	36.0	36.0	36.0	32.0	36.0
28	33.87230253639726	36.0	36.0	36.0	32.0	36.0
29	33.8303976243233	36.0	36.0	36.0	32.0	36.0
30	33.78543044617244	36.0	36.0	36.0	32.0	36.0
31	33.81294173948885	36.0	36.0	36.0	32.0	36.0
32	33.74601241504079	36.0	36.0	36.0	32.0	36.0
33	33.69510811984608	36.0	36.0	36.0	27.0	36.0
34	33.684222137389796	36.0	36.0	36.0	27.0	36.0
35	33.6297528056941	36.0	36.0	36.0	27.0	36.0
36	33.585809149176896	36.0	36.0	36.0	27.0	36.0
37	33.57474059259136	36.0	36.0	36.0	27.0	36.0
38	32.95309712113794	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	0.0
7	2.0
8	2.0
9	4.0
10	14.0
11	16.0
12	10.0
13	11.0
14	225.0
15	491.0
16	651.0
17	778.0
18	975.0
19	1266.0
20	1664.0
21	2424.0
22	3581.0
23	5270.0
24	7864.0
25	11650.0
26	16424.0
27	23055.0
28	32478.0
29	42950.0
30	56790.0
31	76073.0
32	101833.0
33	148835.0
34	313297.0
35	597354.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.31533546259517	17.730362890746434	11.864691941323212	25.08960970533518
2	15.871008186189272	20.621295162945366	38.574727086661795	24.932969564203567
3	18.31670259790831	25.759899074364185	29.024074277726715	26.899324050000793
4	12.155364031575177	16.05198257749598	36.75019675700199	35.042456633926854
5	13.814933201289639	37.48563264097993	33.68573416340131	15.01369999432913
6	32.389545651328994	36.43219625140562	17.718201194758993	13.460056902506393
7	28.756393552366966	31.623084008995928	21.738147204541118	17.882375234095996
8	27.86906900011964	32.95460596701344	20.19373671751328	18.982588315353638
9	27.855129894155194	14.237146305113816	19.11008758735395	38.79763621337704
10	15.821427929254996	27.33664734415781	31.962361910452554	24.87956281613464
11	36.41215419024395	21.800762679863954	22.67617088787057	19.11091224202153
12	24.490040091372645	24.653808196289354	29.3983112797357	21.4578404326023
13	29.68094959118359	20.170374177162305	25.526107739948362	24.62256849170574
14	23.64203132305462	20.268965498161617	26.167974791055144	29.921028387728622
15	24.766020262334003	27.523142031407588	23.7775985412669	23.933239164991512
16	24.70421424668525	26.29709297902214	25.05460606857056	23.94408670572205
17	23.14215140026465	26.28078890993995	26.377766756519893	24.19929293327551
18	24.218808894938174	25.030136918087752	27.72755534438388	23.02349884259019
19	24.76771770629448	25.68986146749016	26.881808184691568	22.660612641523787
20	25.24802908086941	24.28343888473538	26.83078140312705	23.63775063126816
21	26.610751391196214	24.49729506915521	25.37662237062878	23.51533116901979
22	25.022495153426977	24.99725078447741	26.14230769496779	23.83794636712782
23	23.48161084501928	24.875693808033475	26.618020853146128	25.02467449380112
24	23.731904983569322	25.865645389207558	26.548879456013434	23.853570171209686
25	24.253937819390146	24.97558316801824	26.476492055191724	24.29398695739989
26	23.559736820990132	25.872375334438214	27.079465562893752	23.488422281677902
27	24.53293845549463	25.70602165649501	26.00494657806353	23.756093309946827
28	23.445665374730616	25.443463273882145	26.975597070873896	24.135274280513343
29	23.44361461816079	25.86307543549741	26.95353080401544	23.739779142326352
30	23.713428533489726	25.856123645859846	27.145254008476172	23.28519381217426
31	23.96127774853775	25.682020794156113	25.976234657341823	24.380466799964314
32	23.440693334107944	26.005216189691698	26.057225930452187	24.49686454574817
33	23.295302858233658	25.487703495087345	26.527362991386426	24.689630655292564
34	23.823343883490846	25.79264513844315	26.838023726787807	23.54598725127819
35	24.789067914667097	25.61833985139646	26.55185007394209	23.040742159994355
36	23.608291561733317	26.410971676468897	26.1631433731348	23.81759338866298
37	24.284390281015057	26.33891951335911	26.050088876285592	23.326601329340242
38	23.462154001076158	26.268322675508305	26.335824539417352	23.933698783998185
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1153.0
1	893.5
2	634.0
3	634.0
4	1450.0
5	2266.0
6	2266.0
7	2598.5
8	2931.0
9	2827.5
10	2724.0
11	2724.0
12	3459.5
13	4195.0
14	5074.5
15	5954.0
16	5954.0
17	8900.5
18	11847.0
19	11847.0
20	13607.5
21	15368.0
22	15417.0
23	15466.0
24	15466.0
25	17502.0
26	19538.0
27	19538.0
28	24671.0
29	29804.0
30	35774.5
31	41745.0
32	41745.0
33	49793.5
34	57842.0
35	57842.0
36	63163.0
37	68484.0
38	77042.0
39	85600.0
40	85600.0
41	91017.5
42	96435.0
43	106344.5
44	116254.0
45	116254.0
46	122425.0
47	128596.0
48	128596.0
49	130359.0
50	132122.0
51	128527.5
52	124933.0
53	124933.0
54	117041.0
55	109149.0
56	109149.0
57	105175.5
58	101202.0
59	89876.5
60	78551.0
61	78551.0
62	73929.0
63	69307.0
64	56900.5
65	44494.0
66	44494.0
67	37241.0
68	29988.0
69	29988.0
70	24399.0
71	18810.0
72	14822.5
73	10835.0
74	10835.0
75	8198.0
76	5561.0
77	5561.0
78	5293.5
79	5026.0
80	3966.0
81	2906.0
82	2906.0
83	2945.0
84	2984.0
85	2984.0
86	1988.0
87	992.0
88	859.5
89	727.0
90	727.0
91	556.5
92	386.0
93	324.0
94	262.0
95	262.0
96	243.5
97	225.0
98	225.0
99	458.5
100	692.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11542281125431193
2	0.05179849348680625
3	0.011825824280699424
4	0.002904588419820911
5	1.3831373427718624E-4
6	4.149412028315588E-4
7	0.0
8	3.4578433569296563E-4
9	2.074706014157794E-4
10	4.149412028315588E-4
11	0.0023513334827121664
12	0.002835431552682318
13	0.015836922574737827
14	0.010511843805066156
15	0.024204903498507593
16	0.012309922350669576
17	0.02053958954016216
18	0.006016647441057602
19	0.00816051032235399
20	0.006154961175334788
21	0.007607255385245244
22	0.008367980923769768
23	0.01127256934359068
24	0.015007040169074708
25	0.018257412924588583
26	0.019363922798806076
27	0.009681961399403038
28	0.0066390592453049405
29	0.011756667413560832
30	0.0035961570912068424
31	0.006984843580997906
32	0.007261471049552278
33	0.008990392728017106
34	0.013554745959164255
35	0.018119099190311398
36	0.015836922574737827
37	0.010926785007897713
38	0.007054000448136499
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1445988.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.17618211205887
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.14811094623833	43.119144012830276
2	13.442565969759137	14.834189360015907
3	4.158570875719459	6.883621918938028
4	1.6346632339457106	3.607779051523025
5	0.7784326118224832	2.147546977594148
6	0.4506735219777675	1.4919866593036957
7	0.2817103802186163	1.0880592269259803
8	0.18190471576773848	0.8029446179394436
9	0.126327955070231	0.6273264829319151
>10	0.6388119821519791	6.523102107256438
>50	0.06906661649713049	2.6893062858141263
>100	0.07583109882292348	8.772782461019112
>500	0.009431668873924062	3.5042582560741233
>1k	0.003646911964583971	3.0210462786608963
>5k	2.5151116997130835E-4	0.8869063031728698
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	7368	0.5095477970771541	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	5414	0.3744152786883432	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	3079	0.21293399391972825	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	3065	0.2119657977797879	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	2339	0.16175791223716934	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2146	0.14841063687942085	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1917	0.13257371430468304	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1636	0.11314063463873836	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1633	0.11293316403732256	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1549	0.10712398719768075	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1474	0.10193722216228628	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1447	0.10006998674954426	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.915686713859312E-5
2	0.0	0.0	0.0	0.0	6.915686713859312E-5
3	0.0	0.0	0.0	0.0	6.915686713859312E-5
4	0.0	0.0	0.0	0.0	6.915686713859312E-5
5	0.0	0.0	0.0	0.0	6.915686713859312E-5
6	0.0	0.0	0.0	0.0	1.3831373427718624E-4
7	0.0	0.0	0.0	6.915686713859312E-5	1.3831373427718624E-4
8	0.0	0.0	0.0	6.915686713859312E-5	1.3831373427718624E-4
9	0.0	0.0	0.0	6.915686713859312E-5	1.3831373427718624E-4
10	0.0	0.0	0.0	6.915686713859312E-5	1.3831373427718624E-4
11	0.0	0.0	0.0	6.915686713859312E-5	1.3831373427718624E-4
12	0.0	0.0	0.0	6.915686713859312E-5	3.4578433569296563E-4
13	0.0	0.0	0.0	1.3831373427718624E-4	3.4578433569296563E-4
14	0.0	0.0	0.0	1.3831373427718624E-4	3.4578433569296563E-4
15	0.0	0.0	0.0	1.3831373427718624E-4	4.840980699701519E-4
16	0.0	0.0	0.0	2.074706014157794E-4	4.840980699701519E-4
17	0.0	0.0	0.0	2.074706014157794E-4	4.840980699701519E-4
18	0.0	0.0	0.0	2.074706014157794E-4	4.840980699701519E-4
19	0.0	0.0	0.0	3.4578433569296563E-4	4.840980699701519E-4
20	0.0	0.0	0.0	5.53254937108745E-4	6.224118042473381E-4
21	0.0	0.0	0.0	6.224118042473381E-4	6.915686713859313E-4
22	0.0	0.0	0.0	9.681961399403038E-4	6.915686713859313E-4
23	0.0	0.0	0.0	0.002074706014157794	6.915686713859313E-4
24	0.0	0.0	0.0	0.0040110982940384015	6.915686713859313E-4
25	0.0	0.0	0.0	0.004840980699701519	6.915686713859313E-4
26	0.0	0.0	0.0	0.006293274909611975	7.607255385245244E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	375	0.0	18.346012	28
AGTGTCG	45	0.008851402	17.77776	10
CTAGCGG	380	0.0	17.68358	29
TAGATCG	55	0.0013667853	17.450905	5
CATCGTT	335	0.0	17.193417	28
TAGCGGC	400	0.0	16.7994	30
CAAGACG	390	0.0	16.405699	4
CGAGCCG	370	0.0	16.00109	15
AAGACGG	410	0.0	15.996663	5
CGGCATC	345	0.0	15.767552	25
TCGTTTA	380	0.0	15.578392	30
CGTTTAT	395	0.0	15.391855	31
AGGCCCG	375	0.0	15.3599825	10
GGCATCG	355	0.0	15.323397	26
ATCGTTT	390	0.0	15.1789465	29
GTATTAG	285	0.0	15.171522	1
AGCGGCG	480	0.0	14.999465	31
GCATCGT	385	0.0	14.959988	27
ACCGTCG	300	0.0	14.932284	8
TTCGCGT	65	0.0041632564	14.768704	31
>>END_MODULE