FastQCFastQC Report
Thu 2 Feb 2017
SRR4062867_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062867_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1445988
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT82370.5696451146205916No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT64640.447029989183866No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT39190.27102576231614645No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA29350.20297540505177084No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC28930.20007081663194992No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25260.17469024639208625No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG17890.1237216353109431No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA17450.12067873315684502No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA16650.11514618378575756No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC15760.10899122261042278No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA15330.10601747732346327No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA14870.10283626143508798No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC14750.10200637902942486No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG14710.1017297515608705No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG14660.10138396722517752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTA2600.021.53967310
ACCGTCG2550.021.3360088
CCGTCGT2600.020.9249789
TACCGTC2650.020.5315867
ATACCGT2900.019.3134356
GTCGTAG3000.018.66707211
CTAGCGG3050.018.35978129
TCTAGCG3150.018.28484528
TAGCGGC3300.016.9688930
GGTCGTA854.98798E-616.9403729
CGTAAAC854.98798E-616.9403732
ATCGTTT2650.016.90485629
AGGCCCG5350.016.1504410
TAGGACG2900.016.0014514
CGCGGTT4150.015.42255310
GTTTTCG3750.015.3592728
CGAGCCG5350.015.25161115
GTATTAC951.4321391E-515.1587461
GGACTAT1603.0377123E-1015.00246
TTTTCGG4100.014.82856429
Produced by FastQC (version 0.11.5)