##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062867_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1445988
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.11638616641355	32.0	32.0	32.0	32.0	32.0
2	31.273859810731487	32.0	32.0	32.0	32.0	32.0
3	31.368633073026885	32.0	32.0	32.0	32.0	32.0
4	31.46938148864306	32.0	32.0	32.0	32.0	32.0
5	31.384664326398283	32.0	32.0	32.0	32.0	32.0
6	34.90121633097923	36.0	36.0	36.0	36.0	36.0
7	34.93457829525556	36.0	36.0	36.0	36.0	36.0
8	34.874554283991294	36.0	36.0	36.0	32.0	36.0
9	34.98894389165055	36.0	36.0	36.0	36.0	36.0
10	34.833134161555975	36.0	36.0	36.0	32.0	36.0
11	35.01093300912594	36.0	36.0	36.0	36.0	36.0
12	34.90816106357729	36.0	36.0	36.0	32.0	36.0
13	34.949989903097396	36.0	36.0	36.0	36.0	36.0
14	34.90498676337563	36.0	36.0	36.0	32.0	36.0
15	34.86787027278235	36.0	36.0	36.0	32.0	36.0
16	34.88752257971712	36.0	36.0	36.0	32.0	36.0
17	34.85139710702993	36.0	36.0	36.0	32.0	36.0
18	34.84484518543722	36.0	36.0	36.0	32.0	36.0
19	34.830320168632106	36.0	36.0	36.0	32.0	36.0
20	34.81606486360883	36.0	36.0	36.0	32.0	36.0
21	34.79670509022205	36.0	36.0	36.0	32.0	36.0
22	34.77934533343292	36.0	36.0	36.0	32.0	36.0
23	34.725726631203024	36.0	36.0	36.0	32.0	36.0
24	34.699580494443936	36.0	36.0	36.0	32.0	36.0
25	34.679899141624965	36.0	36.0	36.0	32.0	36.0
26	34.61639031582558	36.0	36.0	36.0	32.0	36.0
27	34.598903310400914	36.0	36.0	36.0	32.0	36.0
28	34.5687626729959	36.0	36.0	36.0	32.0	36.0
29	34.52508803669187	36.0	36.0	36.0	32.0	36.0
30	34.501759350700006	36.0	36.0	36.0	32.0	36.0
31	34.483863628190555	36.0	36.0	36.0	32.0	36.0
32	34.445209088872105	36.0	36.0	36.0	32.0	36.0
33	34.396954193257486	36.0	36.0	36.0	32.0	36.0
34	34.35983977737021	36.0	36.0	36.0	32.0	36.0
35	34.31313122930481	36.0	36.0	36.0	32.0	36.0
36	34.29283783821166	36.0	36.0	36.0	32.0	36.0
37	34.25759065773713	36.0	36.0	36.0	32.0	36.0
38	33.83459890400197	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	11.0
21	25.0
22	116.0
23	350.0
24	945.0
25	2412.0
26	5108.0
27	9841.0
28	17642.0
29	27869.0
30	42269.0
31	60982.0
32	86774.0
33	138814.0
34	327554.0
35	725273.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.66108662754829	18.930704600973254	12.658278625711336	25.74993014576711
2	15.036373079426024	21.673154737905666	37.748264680111234	25.54220750255708
3	17.284099176480026	26.355612909650706	29.541946406194242	26.81834150767503
4	11.65253328870404	16.96136753744504	36.773108453332185	34.61299072051873
5	13.334273866726418	37.94817107749166	33.51244961922229	15.205105436559641
6	32.3925681161505	37.01107756230005	17.203486064180428	13.39286825736902
7	28.332510363199543	31.79859776954347	21.837616235886752	18.03127563137024
8	27.68258938852516	33.326786370052346	19.977897083120382	19.012727158302116
9	27.611154759072477	14.035570629471225	18.99935819053995	39.35391642091635
10	15.496086385823297	27.52911870028451	32.12621492166547	24.848579992226714
11	36.467748572693225	21.76318637808155	22.631500686421308	19.137564362803918
12	24.173263088300992	24.864971030148286	29.478155390162836	21.483610491387882
13	29.611282543537765	20.243280363710298	25.529776913469426	24.61566017928251
14	23.440166861689033	20.25058299238998	26.248419765585883	30.06083038033511
15	24.662168704027973	27.60721389112496	23.635811638824112	24.094805766022954
16	24.54980577280432	26.312338907611206	25.065578044615894	24.072277274968584
17	22.97771489113326	26.398144382940934	26.378088891470746	24.246051834455056
18	24.121638630472727	24.83492255814018	27.983427248358904	23.060011563028183
19	24.74183741497163	25.633131118653818	26.967097928890144	22.657933537484404
20	25.175036030727778	24.247642442399243	26.98016857677934	23.59715295009364
21	26.46139035924276	24.568910072435003	25.520094938678522	23.449604629643716
22	24.911634751903026	24.893723110927	26.251135037866867	23.943507099303105
23	23.365892386382185	24.627936054794368	26.890887061303413	25.115284497520037
24	23.735466684370824	25.783962245883092	26.67484100836245	23.805730061383635
25	24.130869870109393	24.870711058060035	26.677367554042707	24.32105151778786
26	23.555467649432533	25.77160098949714	27.146647929753172	23.526283431317154
27	24.55858870100492	25.42339592264661	26.185021027159994	23.83299434918848
28	23.35966826833971	25.205119267933068	27.230862220156737	24.204350243570484
29	23.427787782471224	25.52296422930204	27.267031261670223	23.782216726556513
30	23.70517597656412	25.478565520599066	27.316409264807177	23.49984923802964
31	23.929175069226023	25.367153807638793	26.26418753129348	24.4394835918417
32	23.47557517766399	25.76625808789561	26.26038390360086	24.497782830839537
33	23.355311385709978	25.147857381942313	26.759212386271532	24.737618846076177
34	23.738786213993475	25.561415447431102	27.10790131038432	23.591897028191106
35	24.847509107959404	25.45823340166032	26.570759923318864	23.123497567061413
36	23.62751281476748	26.135970699618532	26.389845558884307	23.846670926729683
37	24.456288710556382	26.073314577990963	26.183550624209882	23.286846087242772
38	23.526922114045366	25.796446161996034	26.590298937887365	24.086332786071235
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	839.0
1	595.5
2	352.0
3	352.0
4	1299.0
5	2246.0
6	2246.0
7	2526.0
8	2806.0
9	2868.0
10	2930.0
11	2930.0
12	3689.0
13	4448.0
14	5240.0
15	6032.0
16	6032.0
17	9312.5
18	12593.0
19	12593.0
20	14059.5
21	15526.0
22	15828.0
23	16130.0
24	16130.0
25	17725.0
26	19320.0
27	19320.0
28	24332.0
29	29344.0
30	35627.0
31	41910.0
32	41910.0
33	50354.5
34	58799.0
35	58799.0
36	63679.0
37	68559.0
38	77216.5
39	85874.0
40	85874.0
41	90657.0
42	95440.0
43	106209.0
44	116978.0
45	116978.0
46	122951.5
47	128925.0
48	128925.0
49	131685.5
50	134446.0
51	130028.0
52	125610.0
53	125610.0
54	117227.0
55	108844.0
56	108844.0
57	104866.0
58	100888.0
59	89321.0
60	77754.0
61	77754.0
62	73508.5
63	69263.0
64	56956.5
65	44650.0
66	44650.0
67	37255.5
68	29861.0
69	29861.0
70	24206.5
71	18552.0
72	14496.5
73	10441.0
74	10441.0
75	7833.5
76	5226.0
77	5226.0
78	5068.5
79	4911.0
80	3829.0
81	2747.0
82	2747.0
83	2614.0
84	2481.0
85	2481.0
86	1580.5
87	680.0
88	558.0
89	436.0
90	436.0
91	254.0
92	72.0
93	53.5
94	35.0
95	35.0
96	26.5
97	18.0
98	18.0
99	20.0
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008298824056631175
2	6.915686713859312E-5
3	0.0
4	1.3831373427718624E-4
5	0.0
6	1.3831373427718624E-4
7	4.149412028315588E-4
8	0.001728921678464828
9	0.005255921902533078
10	6.915686713859313E-4
11	0.00574001997250323
12	1.3831373427718624E-4
13	1.3831373427718624E-4
14	0.0
15	0.0
16	6.915686713859312E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	1.3831373427718624E-4
22	6.915686713859312E-5
23	0.0
24	0.0
25	1.3831373427718624E-4
26	6.915686713859312E-5
27	6.915686713859312E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.3831373427718624E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1445988.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.85466613069858
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.30366328357465	40.33004647402582
2	14.517918013000319	15.346794189799736
3	4.553402362514788	7.2200568488836
4	1.7597629146659244	3.7204672529540974
5	0.8888240882039088	2.3489250215470094
6	0.4854663009793825	1.5395495553582292
7	0.308351572293781	1.1408473583124634
8	0.1923315496684335	0.813249587529994
9	0.13089931106341718	0.6226775444695837
>10	0.6875235716789914	6.682433123046273
>50	0.07450693480768282	2.7743642961512855
>100	0.08197909107328984	9.15006077459394
>500	0.01090421826887477	3.8846624967820307
>1k	0.004204035959084249	3.4050513880791584
>5k	2.6275224744276554E-4	1.0208140884669068
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	8237	0.5696451146205916	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	6464	0.447029989183866	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	3919	0.27102576231614645	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2935	0.20297540505177084	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	2893	0.20007081663194992	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2526	0.17469024639208625	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1789	0.1237216353109431	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1745	0.12067873315684502	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1665	0.11514618378575756	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1576	0.10899122261042278	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1533	0.10601747732346327	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1487	0.10283626143508798	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1475	0.10200637902942486	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1471	0.1017297515608705	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1466	0.10138396722517752	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	6.915686713859312E-5	0.0
10	0.0	0.0	0.0	1.3831373427718624E-4	0.0
11	0.0	0.0	0.0	1.3831373427718624E-4	0.0
12	0.0	0.0	0.0	1.3831373427718624E-4	3.4578433569296563E-4
13	0.0	0.0	0.0	1.3831373427718624E-4	4.149412028315588E-4
14	0.0	0.0	0.0	1.3831373427718624E-4	4.149412028315588E-4
15	0.0	0.0	0.0	1.3831373427718624E-4	6.224118042473381E-4
16	0.0	0.0	0.0	2.766274685543725E-4	6.224118042473381E-4
17	0.0	0.0	0.0	2.766274685543725E-4	6.224118042473381E-4
18	0.0	0.0	0.0	2.766274685543725E-4	6.915686713859313E-4
19	0.0	0.0	0.0	4.149412028315588E-4	6.915686713859313E-4
20	0.0	0.0	0.0	5.53254937108745E-4	7.607255385245244E-4
21	0.0	0.0	0.0	6.915686713859313E-4	8.298824056631176E-4
22	0.0	0.0	0.0	0.0011756667413560832	8.298824056631176E-4
23	0.0	0.0	0.0	0.00221301974843498	8.298824056631176E-4
24	0.0	0.0	0.0	0.004080255161176994	8.298824056631176E-4
25	0.0	0.0	0.0	0.004910137566840112	8.298824056631176E-4
26	0.0	0.0	0.0	0.006224118042473381	8.298824056631176E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCGTA	260	0.0	21.539673	10
ACCGTCG	255	0.0	21.336008	8
CCGTCGT	260	0.0	20.924978	9
TACCGTC	265	0.0	20.531586	7
ATACCGT	290	0.0	19.313435	6
GTCGTAG	300	0.0	18.667072	11
CTAGCGG	305	0.0	18.359781	29
TCTAGCG	315	0.0	18.284845	28
TAGCGGC	330	0.0	16.96889	30
GGTCGTA	85	4.98798E-6	16.94037	29
CGTAAAC	85	4.98798E-6	16.94037	32
ATCGTTT	265	0.0	16.904856	29
AGGCCCG	535	0.0	16.15044	10
TAGGACG	290	0.0	16.001451	4
CGCGGTT	415	0.0	15.422553	10
GTTTTCG	375	0.0	15.35927	28
CGAGCCG	535	0.0	15.251611	15
GTATTAC	95	1.4321391E-5	15.158746	1
GGACTAT	160	3.0377123E-10	15.0024	6
TTTTCGG	410	0.0	14.828564	29
>>END_MODULE