FastQCFastQC Report
Thu 2 Feb 2017
SRR4062866_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062866_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209875
Sequences flagged as poor quality0
Sequence length38
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT27351.3031566408576534No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT19460.9272185824895772No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA15240.7261465157832042No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC12780.6089338892197736No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT10740.5117331745086361No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9840.46885050625372243No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA8550.40738534842167956No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA7020.3344848123883264No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG6320.301131625967838No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6090.29017272185824894No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCT5780.2754020250148898No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTA5440.2592019058963669No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG5120.2439547349612865No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA5060.2410958904109589No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAAC5030.23966646813579512No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA4850.2310899344848124No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA4810.22918403811792734No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA4770.22727814175104227No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4190.19964264443120905No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGG4100.19535437760571767No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA4060.19344848123883263No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGT4000.19058963668850507No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGT3850.18344252531268612No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3850.18344252531268612No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTA3840.18296605122096488No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCT3820.18201310303752233No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGG3710.17677188802858845No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGAT3680.17534246575342466No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGC3680.17534246575342466No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC3600.17153067301965455No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTA3540.168671828469327No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3450.16438356164383564No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGC3440.16390708755211436No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA3430.1634306134603931No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG3380.16104824300178677No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCT3340.15914234663490173No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTC3310.15771292435973794No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGT3300.15723645026801666No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG3270.1558070279928529No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCA3270.1558070279928529No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTTCT3210.1529481834425253No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAG3160.150565812983919No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATG3120.14865991661703395No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT3100.14770696843359143No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCT2940.14008338296605122No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAG2930.13960690887432997No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCG2870.13674806432400238No Hit
GTATCAACGCAGAGTACATGGGAGAAATCGTAAATAGA2840.1353186420488386No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA2830.13484216795711731No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACC2820.13436569386539607No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC2810.13388921977367482No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA2790.13293627159023227No Hit
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTT2760.1315068493150685No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC2700.12864800476474092No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACA2700.12864800476474092No Hit
GTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTA2650.1262656343061346No Hit
CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC2600.12388326384752829No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC2590.12340678975580703No Hit
CTATTATATAAATCAAAACATTTATCCTACTAAAAGTA2500.11911852293031568No Hit
GACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGG2500.11911852293031568No Hit
CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAA2490.1186420488385944No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA2460.11721262656343061No Hit
ATTATAACCTAGACTTACAAGTCAAAGTAAAATCAACA2400.11435378201310303No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCC2380.11340083382966051No Hit
CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCA2380.11340083382966051No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCAT2350.11197141155449672No Hit
GTTTAGATTATAGCCAAAAGAGGGACAGCTCTTCTGGA2330.11101846337105419No Hit
ATTTAAGAGTTCATATCGACAATTAGGGTTTACGACCT2330.11101846337105419No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTC2320.11054198927933294No Hit
GGTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGG2300.1095890410958904No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTG2260.10768314472900536No Hit
CCATTAATAGCTTCTACACCATTGGGATGTCCTGATCC2260.10768314472900536No Hit
TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTC2230.10625372245384157No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA2220.1057772483621203No Hit
GTATAAGTTTGAAATTTCGGTTGGGGTGACCTCGGAGA2210.10530077427039905No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA2200.10482430017867779No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTA2190.10434782608695652No Hit
CCTTTAATAGTGAATAATTAACAAAACAGCTTTTAACC2190.10434782608695652No Hit
AGCATGAACGGCTAAACGAGGGTCCAACTGTCTCTTAT2180.10387135199523526No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCT2170.10339487790351401No Hit
CTATAGTATAAGTTTGAAATTTCGGTTGGGGTGACCTC2170.10339487790351401No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG2160.10291840381179272No Hit
CTTAAATAGGATTGCGCTGTTATCCCTAGGGTAACTTG2150.10244192972007148No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTAT2150.10244192972007148No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCA2150.10244192972007148No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG2100.10005955926146515No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATGTG200.003747404331.998575
CGGTGTT356.407979E-527.42734514
TCCGGTG356.407979E-527.42734512
TTGGGTA605.9353624E-926.66547628
GTAACCA557.540439E-826.18064932
TTAGGCA451.2322311E-524.887784
CCGGTGT451.2322311E-524.8877813
GACCTTT451.2322311E-524.8877819
ATTGAAC651.3911631E-824.6142858
TATCACC401.5879887E-423.9989283
TTCCGGT401.5879887E-423.99892811
ATCACCA401.5879887E-423.9989284
AGGCATT401.5879887E-423.9989286
TAGGCAT401.5879887E-423.9989285
GTCCTAT350.00204724222.8833851
GGTAACC703.0553565E-822.85612131
GAACTTA703.0553565E-822.85612111
TGGGTAA703.0553565E-822.85612129
GACCAAG703.0553565E-822.85612119
TTGAACT703.0553565E-822.8561219
Produced by FastQC (version 0.11.5)