##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062866_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	209875
Sequences flagged as poor quality	0
Sequence length	38
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.799466349017273	32.0	32.0	32.0	32.0	32.0
2	30.61461346039309	32.0	32.0	32.0	32.0	32.0
3	30.747683144729006	32.0	32.0	32.0	32.0	32.0
4	30.701303156640858	32.0	32.0	32.0	32.0	32.0
5	30.79301012507445	32.0	32.0	32.0	32.0	32.0
6	34.2944228707564	36.0	36.0	36.0	32.0	36.0
7	34.112690887433	36.0	36.0	36.0	32.0	36.0
8	34.05042525312686	36.0	36.0	36.0	32.0	36.0
9	34.08872424061941	36.0	36.0	36.0	32.0	36.0
10	34.00011911852293	36.0	36.0	36.0	32.0	36.0
11	34.29630970815962	36.0	36.0	36.0	32.0	36.0
12	34.12083859440143	36.0	36.0	36.0	32.0	36.0
13	34.20946277546158	36.0	36.0	36.0	32.0	36.0
14	34.12504586063133	36.0	36.0	36.0	32.0	36.0
15	34.13533293627159	36.0	36.0	36.0	32.0	36.0
16	34.13430851697439	36.0	36.0	36.0	32.0	36.0
17	34.07826086956522	36.0	36.0	36.0	32.0	36.0
18	34.10364264443121	36.0	36.0	36.0	32.0	36.0
19	34.06643001786778	36.0	36.0	36.0	32.0	36.0
20	33.970739726027396	36.0	36.0	36.0	32.0	36.0
21	33.875973793924956	36.0	36.0	36.0	32.0	36.0
22	33.891044669446096	36.0	36.0	36.0	32.0	36.0
23	33.92947230494342	36.0	36.0	36.0	32.0	36.0
24	33.90143656938654	36.0	36.0	36.0	32.0	36.0
25	33.867278141751044	36.0	36.0	36.0	32.0	36.0
26	33.87603097081596	36.0	36.0	36.0	32.0	36.0
27	33.84654675402025	36.0	36.0	36.0	32.0	36.0
28	33.81350327575938	36.0	36.0	36.0	27.0	36.0
29	33.76347826086957	36.0	36.0	36.0	27.0	36.0
30	33.680900536033356	36.0	36.0	36.0	27.0	36.0
31	33.70935080405003	36.0	36.0	36.0	27.0	36.0
32	33.63896605122096	36.0	36.0	36.0	27.0	36.0
33	33.58410005955926	36.0	36.0	36.0	27.0	36.0
34	33.481720071471116	36.0	36.0	36.0	21.0	36.0
35	33.36054318046456	36.0	36.0	36.0	21.0	36.0
36	33.307787969029185	36.0	36.0	36.0	21.0	36.0
37	33.25292197736748	36.0	36.0	36.0	21.0	36.0
38	32.55252888624181	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	0.0
7	0.0
8	1.0
9	1.0
10	1.0
11	3.0
12	1.0
13	1.0
14	129.0
15	200.0
16	257.0
17	283.0
18	343.0
19	407.0
20	508.0
21	623.0
22	779.0
23	1052.0
24	1339.0
25	1770.0
26	2359.0
27	3197.0
28	4583.0
29	6109.0
30	8088.0
31	10674.0
32	14128.0
33	20077.0
34	43198.0
35	89763.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.00980368771319	22.017031223910504	13.242372921785178	21.730792166591133
2	12.397919536996868	23.24693341469577	43.712129518833336	20.64301752947402
3	16.819557292287165	30.837522933593842	32.11179680240177	20.23112297171722
4	9.079993138544227	19.619475098633426	41.55659748032096	29.74393428250138
5	8.69374627754616	43.10661107802263	36.56081000595592	11.638832638475282
6	25.022632627195364	42.59984562165871	20.42749459199329	11.950027159152635
7	23.82429457674605	36.80732248873134	23.43548986534778	15.932893069174838
8	25.19654649932817	36.77730447792485	21.581996817137902	16.44415220560908
9	27.193935408864366	15.906210392902409	23.026673146745193	33.87318105148804
10	15.067611995768889	29.018363398991774	35.40981731896281	20.504207286276525
11	29.945489545809746	25.21870890273886	27.653096231917207	17.182705319534183
12	23.012303088637513	29.63414750364518	32.00756673305823	15.345982674659068
13	26.89175343706069	24.173556672782293	29.39121732707475	19.543472563082275
14	20.467086641188637	26.177723560189463	29.73305250316887	23.62213729545303
15	21.684943976589793	32.72043579588511	28.626509009975077	16.96811121755003
16	19.30474148201096	29.96568977841315	31.484870145341908	19.244698594233977
17	17.060082257457264	30.45813495751302	32.062774926249475	20.419007858780244
18	17.865855982922273	27.753211603705257	36.74284298402775	17.63808942934472
19	20.421233203087773	29.824168493281235	32.106642523587155	17.64795578004384
20	20.165724795822097	28.988973916689698	34.21850132941972	16.62679995806848
21	21.640244163938643	27.284510075812808	30.104498734864837	20.97074702538371
22	20.138663871152197	31.155055751453347	30.20918707709902	18.497093300295436
23	18.760364447324783	30.745477765282196	32.10643691744658	18.38772086994644
24	19.654217677025205	31.81092531082762	31.46685855615865	17.067998455988526
25	19.913267251239038	30.062428516965305	32.44614944719786	17.57815478459779
26	16.408286589811897	30.23547297136294	35.115544266154515	18.24069617267065
27	18.274738156277103	32.59680355287861	32.10075384307484	17.027704447769445
28	17.032144933337147	30.683973277677712	33.0069283624477	19.27695342653744
29	18.10466423955931	30.34043039856661	32.95322417703906	18.601681184835027
30	18.904443787940878	29.861911886632424	33.31125575367139	17.92238857175531
31	18.61794893787346	29.529405037596135	32.58522267013561	19.26742335439479
32	15.637567902411131	32.62031830744306	33.16639664538263	18.575717144763175
33	16.863944800243978	32.14489935956084	33.20133806038426	17.789817779810917
34	16.746960967915637	31.3724368963036	35.315673352298994	16.56492878348177
35	18.453319862945154	32.66585018323222	31.98486487516858	16.89596507865404
36	17.176256421498078	31.468438158233337	32.973856520620274	18.381448899648305
37	18.817575777791543	31.992737912006746	30.13194699247583	19.05773931772588
38	17.499594971933938	34.45406981730852	30.59153157789404	17.4548036328635
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	722.0
1	509.5
2	297.0
3	297.0
4	642.5
5	988.0
6	988.0
7	1150.5
8	1313.0
9	1275.5
10	1238.0
11	1238.0
12	1497.0
13	1756.0
14	2122.5
15	2489.0
16	2489.0
17	3409.0
18	4329.0
19	4329.0
20	4854.5
21	5380.0
22	5681.5
23	5983.0
24	5983.0
25	6691.5
26	7400.0
27	7400.0
28	9599.0
29	11798.0
30	13959.0
31	16120.0
32	16120.0
33	18175.5
34	20231.0
35	20231.0
36	19970.5
37	19710.0
38	20190.5
39	20671.0
40	20671.0
41	19158.5
42	17646.0
43	17517.0
44	17388.0
45	17388.0
46	18021.0
47	18654.0
48	18654.0
49	16040.0
50	13426.0
51	9907.0
52	6388.0
53	6388.0
54	5501.0
55	4614.0
56	4614.0
57	3821.0
58	3028.0
59	2633.5
60	2239.0
61	2239.0
62	2127.0
63	2015.0
64	1685.5
65	1356.0
66	1356.0
67	1200.0
68	1044.0
69	1044.0
70	871.5
71	699.0
72	519.0
73	339.0
74	339.0
75	274.0
76	209.0
77	209.0
78	173.0
79	137.0
80	103.5
81	70.0
82	70.0
83	69.5
84	69.0
85	69.0
86	46.5
87	24.0
88	19.5
89	15.0
90	15.0
91	10.5
92	6.0
93	6.0
94	6.0
95	6.0
96	8.0
97	10.0
98	10.0
99	39.0
100	68.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12388326384752829
2	0.05431804645622395
3	0.01429422275163788
4	0.0033353186420488385
5	0.0
6	4.764740917212627E-4
7	4.764740917212627E-4
8	4.764740917212627E-4
9	4.764740917212627E-4
10	4.764740917212627E-4
11	0.0033353186420488385
12	0.004288266825491363
13	0.01429422275163788
14	0.010005955926146516
15	0.024776652769505657
16	0.011911852293031567
17	0.021917808219178082
18	0.005241215008933889
19	0.00714711137581894
20	0.004288266825491363
21	0.007623585467540203
22	0.00714711137581894
23	0.010958904109589041
24	0.015247170935080406
25	0.016676593210244194
26	0.02001191185229303
27	0.008100059559261465
28	0.006194163192376414
29	0.010958904109589041
30	0.004288266825491363
31	0.006194163192376414
32	0.00714711137581894
33	0.00905300774270399
34	0.010482430017867778
35	0.015247170935080406
36	0.01572364502680167
37	0.008576533650982727
38	0.006194163192376414
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	209875.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.16938653960691
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.6846596851338	26.544371649791543
2	10.716327583562254	7.323406789755807
3	3.5488684060072795	3.6378796902918404
4	1.737481349267218	2.3747468731387733
5	1.0988244809169885	1.877307921381775
6	0.702801444647414	1.4408576533650983
7	0.5159455049990936	1.2340678975580701
8	0.436462008283017	1.1930911256700416
9	0.3332729072831983	1.024895771292436
>10	2.542077447603642	17.834425253126863
>50	0.3611618534993655	8.827635497319832
>100	0.301200619134606	19.548779035139965
>500	0.01394447310808361	3.0613460393091123
>1k	0.006972236554041805	4.077188802858844
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2735	1.3031566408576534	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1946	0.9272185824895772	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1524	0.7261465157832042	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1278	0.6089338892197736	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1074	0.5117331745086361	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	984	0.46885050625372243	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	855	0.40738534842167956	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAA	702	0.3344848123883264	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	632	0.301131625967838	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	609	0.29017272185824894	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCT	578	0.2754020250148898	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTA	544	0.2592019058963669	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	512	0.2439547349612865	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	506	0.2410958904109589	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAAC	503	0.23966646813579512	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	485	0.2310899344848124	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA	481	0.22918403811792734	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	477	0.22727814175104227	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	419	0.19964264443120905	No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGG	410	0.19535437760571767	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	406	0.19344848123883263	No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGT	400	0.19058963668850507	No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGT	385	0.18344252531268612	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	385	0.18344252531268612	No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTA	384	0.18296605122096488	No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCT	382	0.18201310303752233	No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGG	371	0.17677188802858845	No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGAT	368	0.17534246575342466	No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGC	368	0.17534246575342466	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	360	0.17153067301965455	No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTA	354	0.168671828469327	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	345	0.16438356164383564	No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGC	344	0.16390708755211436	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	343	0.1634306134603931	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG	338	0.16104824300178677	No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCT	334	0.15914234663490173	No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTC	331	0.15771292435973794	No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGT	330	0.15723645026801666	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	327	0.1558070279928529	No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCA	327	0.1558070279928529	No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTTCT	321	0.1529481834425253	No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAG	316	0.150565812983919	No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATG	312	0.14865991661703395	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	310	0.14770696843359143	No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCT	294	0.14008338296605122	No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAG	293	0.13960690887432997	No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCG	287	0.13674806432400238	No Hit
GTATCAACGCAGAGTACATGGGAGAAATCGTAAATAGA	284	0.1353186420488386	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA	283	0.13484216795711731	No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACC	282	0.13436569386539607	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	281	0.13388921977367482	No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA	279	0.13293627159023227	No Hit
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTT	276	0.1315068493150685	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	270	0.12864800476474092	No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACA	270	0.12864800476474092	No Hit
GTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTA	265	0.1262656343061346	No Hit
CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC	260	0.12388326384752829	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	259	0.12340678975580703	No Hit
CTATTATATAAATCAAAACATTTATCCTACTAAAAGTA	250	0.11911852293031568	No Hit
GACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGG	250	0.11911852293031568	No Hit
CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAA	249	0.1186420488385944	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	246	0.11721262656343061	No Hit
ATTATAACCTAGACTTACAAGTCAAAGTAAAATCAACA	240	0.11435378201310303	No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCC	238	0.11340083382966051	No Hit
CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCA	238	0.11340083382966051	No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCAT	235	0.11197141155449672	No Hit
GTTTAGATTATAGCCAAAAGAGGGACAGCTCTTCTGGA	233	0.11101846337105419	No Hit
ATTTAAGAGTTCATATCGACAATTAGGGTTTACGACCT	233	0.11101846337105419	No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTC	232	0.11054198927933294	No Hit
GGTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGG	230	0.1095890410958904	No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTG	226	0.10768314472900536	No Hit
CCATTAATAGCTTCTACACCATTGGGATGTCCTGATCC	226	0.10768314472900536	No Hit
TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTC	223	0.10625372245384157	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA	222	0.1057772483621203	No Hit
GTATAAGTTTGAAATTTCGGTTGGGGTGACCTCGGAGA	221	0.10530077427039905	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	220	0.10482430017867779	No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTA	219	0.10434782608695652	No Hit
CCTTTAATAGTGAATAATTAACAAAACAGCTTTTAACC	219	0.10434782608695652	No Hit
AGCATGAACGGCTAAACGAGGGTCCAACTGTCTCTTAT	218	0.10387135199523526	No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCT	217	0.10339487790351401	No Hit
CTATAGTATAAGTTTGAAATTTCGGTTGGGGTGACCTC	217	0.10339487790351401	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG	216	0.10291840381179272	No Hit
CTTAAATAGGATTGCGCTGTTATCCCTAGGGTAACTTG	215	0.10244192972007148	No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTAT	215	0.10244192972007148	No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCA	215	0.10244192972007148	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG	210	0.10005955926146515	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	4.7647409172126264E-4	0.0
9	0.0	0.0	0.0	4.7647409172126264E-4	0.0
10	0.0	0.0	0.0	9.529481834425253E-4	0.0
11	0.0	0.0	0.0	9.529481834425253E-4	0.0
12	0.0	0.0	0.0	9.529481834425253E-4	0.0
13	0.0	0.0	0.0	9.529481834425253E-4	0.0
14	0.0	0.0	0.0	9.529481834425253E-4	0.0
15	0.0	0.0	0.0	9.529481834425253E-4	0.0
16	0.0	0.0	0.0	0.0014294222751637879	0.0
17	0.0	0.0	0.0	0.0014294222751637879	0.0
18	0.0	0.0	0.0	0.0014294222751637879	0.0
19	0.0	0.0	0.0	0.0014294222751637879	0.0
20	0.0	0.0	0.0	0.0019058963668850506	0.0
21	0.0	0.0	0.0	0.0023823704586063135	0.0
22	0.0	0.0	0.0	0.004764740917212627	0.0
23	0.0	0.0	0.0	0.008100059559261465	0.0
24	0.0	0.0	0.0	0.01620011911852293	0.0
25	0.0	0.0	0.0	0.02144133412745682	0.0
26	0.0	0.0	0.0	0.026206075044669448	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATGTG	20	0.0037474043	31.99857	5
CGGTGTT	35	6.407979E-5	27.427345	14
TCCGGTG	35	6.407979E-5	27.427345	12
TTGGGTA	60	5.9353624E-9	26.665476	28
GTAACCA	55	7.540439E-8	26.180649	32
TTAGGCA	45	1.2322311E-5	24.88778	4
CCGGTGT	45	1.2322311E-5	24.88778	13
GACCTTT	45	1.2322311E-5	24.88778	19
ATTGAAC	65	1.3911631E-8	24.614285	8
TATCACC	40	1.5879887E-4	23.998928	3
TTCCGGT	40	1.5879887E-4	23.998928	11
ATCACCA	40	1.5879887E-4	23.998928	4
AGGCATT	40	1.5879887E-4	23.998928	6
TAGGCAT	40	1.5879887E-4	23.998928	5
GTCCTAT	35	0.002047242	22.883385	1
GGTAACC	70	3.0553565E-8	22.856121	31
GAACTTA	70	3.0553565E-8	22.856121	11
TGGGTAA	70	3.0553565E-8	22.856121	29
GACCAAG	70	3.0553565E-8	22.856121	19
TTGAACT	70	3.0553565E-8	22.856121	9
>>END_MODULE